Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,742 T→A 10.0% intergenic (+554/‑377) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7420T→A10.0% 81.1 ‑3.4 ‑0.1299intergenic (+554/‑377)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/21):  ref base (139/130):  total (148/151)
Fisher's exact test strand distribution p-value = 3.31e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.85e-01

Only 100 of 311 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCC  >  NC_014034/1395642‑1395842
                                                                                                      |                                                                                                          
   gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gc                                                                                                       >  2:4365631/1‑101 (MQ=255)
       gggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaa                                                                                                   <  2:282795/101‑1 (MQ=255)
        ggCGTGGATCGAAACATCTGCATGTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                                  >  1:3951700/1‑101 (MQ=255)
        ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                                  <  1:621574/101‑1 (MQ=255)
         gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAAc                                                                                                 <  1:2661211/101‑1 (MQ=255)
           gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                               >  2:1547835/1‑101 (MQ=255)
            tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..a                                                                                            >  2:1281276/1‑101 (MQ=255)
               aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAg                                                                                         >  2:3603562/1‑101 (MQ=255)
               aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAg                                                                                         >  1:80252/1‑101 (MQ=255)
                 cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTc                                                                                       <  1:594622/101‑1 (MQ=255)
                  gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCt                                                                                      <  2:1869812/101‑1 (MQ=255)
                     aCACCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                 <  2:2702594/101‑1 (MQ=255)
                      cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                                >  2:4056125/1‑101 (MQ=255)
                      cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                                <  1:3632364/101‑1 (MQ=255)
                       agcTGCATGGAGTCGGCGGGGAGTTCGACCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                               <  2:3044277/99‑1 (MQ=255)
                           tgaTGTAGGGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                           <  1:1000128/99‑1 (MQ=255)
                           gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                           >  2:1950133/1‑101 (MQ=255)
                               gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                       <  2:2937660/101‑1 (MQ=255)
                               gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                       >  2:1625582/1‑101 (MQ=255)
                               gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                       <  1:3388345/101‑1 (MQ=255)
                                 aGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                     >  1:2032090/1‑101 (MQ=255)
                                   tCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGGATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                                   >  2:509465/1‑101 (MQ=255)
                                          gCAGTTGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCg                                                            <  2:4077986/101‑1 (MQ=255)
                                           gaGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGt                                                           <  2:1795476/100‑1 (MQ=255)
                                            aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCTTg                                                          >  1:335673/1‑101 (MQ=255)
                                            aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                          >  1:3816219/1‑101 (MQ=255)
                                             gtgcGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                         <  2:1927522/98‑1 (MQ=255)
                                               tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                          >  1:2844909/9‑101 (MQ=2)
                                               tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                          <  2:1696781/93‑1 (MQ=2)
                                               tCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:1547618/1‑101 (MQ=255)
                                                cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  2:829276/101‑1 (MQ=255)
                                                cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCCCGGGGGCGTGGATc                                                      >  1:1668730/1‑101 (MQ=255)
                                                 gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  1:1048791/93‑1 (MQ=0)
                                                 gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     >  2:4114243/1‑101 (MQ=255)
                                                 aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  1:2062223/95‑1 (MQ=2)
                                                  aGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:3010680/1‑101 (MQ=255)
                                                  aGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:2588555/1‑101 (MQ=255)
                                                   gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  1:4283753/97‑1 (MQ=2)
                                                   gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  1:3130107/101‑1 (MQ=255)
                                                     ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:2736164/3‑101 (MQ=2)
                                                       tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:2262273/99‑1 (MQ=2)
                                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                              >  1:4146967/1‑101 (MQ=255)
                                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                              >  1:1396361/1‑101 (MQ=255)
                                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                             <  1:1281369/101‑1 (MQ=255)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCta                                              >  2:1509462/1‑100 (MQ=2)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                            >  2:139541/1‑101 (MQ=255)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGCGGGCGTGGATCGAAACCTgcg                                            <  1:2535964/101‑1 (MQ=255)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAcacc                                              >  2:2742486/1‑98 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                              >  2:1160083/1‑98 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAGTT..CCGCAATCGG..CCGGGCTCGATCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                             >  1:762208/1‑99 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                             <  2:70453/101‑3 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                             <  2:320897/101‑5 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                             <  1:143861/101‑5 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                             <  1:1122108/101‑5 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                            >  1:3061356/1‑96 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                            >  1:1755366/1‑96 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                           >  2:3439305/1‑95 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGt                                        >  2:1103746/1‑101 (MQ=255)
                                                               ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                       <  1:2865686/101‑1 (MQ=255)
                                                                cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                      >  1:1825055/1‑101 (MQ=255)
                                                                  cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                       <  2:2623561/101‑11 (MQ=2)
                                                                  cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGt                                    <  2:2987489/101‑1 (MQ=255)
                                                                   ccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGtt                                   <  1:4072075/101‑1 (MQ=255)
                                                                      cgGGGGCGTGGATCGAAACT.GGTATG.GATATG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                <  1:3221556/101‑1 (MQ=255)
                                                                       gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                               >  2:4292067/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                               >  2:445663/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                               >  1:1904105/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                               <  1:562786/101‑1 (MQ=255)
                                                                        ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                              >  2:3700339/1‑101 (MQ=255)
                                                                        ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                              <  2:3788840/101‑1 (MQ=255)
                                                                          ggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                            <  2:3098349/101‑1 (MQ=255)
                                                                              tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                        <  1:2578805/101‑1 (MQ=255)
                                                                              tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                        <  2:809163/101‑1 (MQ=255)
                                                                               ggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                       <  2:577972/101‑1 (MQ=255)
                                                                               ggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                       <  1:4204488/101‑1 (MQ=255)
                                                                               ggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                      >  2:3760347/1‑101 (MQ=255)
                                                                                 aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                     <  1:229321/101‑1 (MQ=255)
                                                                                  tCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                    <  1:974084/101‑1 (MQ=255)
                                                                                    gAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACTCCg                  >  1:217156/1‑101 (MQ=255)
                                                                                    gAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                  >  1:124044/1‑101 (MQ=255)
                                                                                    gAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg                  >  2:1245130/1‑101 (MQ=255)
                                                                                        cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa              <  1:1146380/101‑1 (MQ=255)
                                                                                        cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa              <  1:3484003/101‑1 (MQ=255)
                                                                                        cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa              <  2:145072/101‑1 (MQ=255)
                                                                                         t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc             >  2:3187226/1‑101 (MQ=255)
                                                                                           ggTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg            >  1:1311420/1‑101 (MQ=255)
                                                                                           ggTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg            >  1:4408695/1‑101 (MQ=255)
                                                                                            gTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCCGTGATAGACGCCGTGGACGt           >  1:824162/1‑101 (MQ=255)
                                                                                             tATG.GATCTG..GCGCAAACGG..AAAGCCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc          <  2:2537744/101‑1 (MQ=255)
                                                                                              aTG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg         >  2:1754223/1‑101 (MQ=255)
                                                                                              aTG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg         >  1:3184218/1‑101 (MQ=255)
                                                                                                g.gATCTG..TCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGAGCGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt       <  2:328784/101‑1 (MQ=255)
                                                                                                g.gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt       <  1:712243/101‑1 (MQ=255)
                                                                                                g.gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt       >  1:507177/1‑101 (MQ=255)
                                                                                                  gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc      <  1:2711685/101‑1 (MQ=255)
                                                                                                  gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc      <  2:335638/101‑1 (MQ=255)
                                                                                                  gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc      >  2:2392933/1‑101 (MQ=255)
                                                                                                  gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc      >  2:860663/1‑101 (MQ=255)
                                                                                                    tCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccc    >  2:3944024/1‑101 (MQ=255)
                                                                                                    tCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccc    <  2:2071203/101‑1 (MQ=255)
                                                                                                      |                                                                                                          
CCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCC  >  NC_014034/1395642‑1395842

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG