Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,866 C→T 20.7% intergenic (+678/‑253) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8660C→T20.7% 150.2 ‑1.2 ‑0.0261intergenic (+678/‑253)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/24):  ref base (98/109):  total (128/133)
Fisher's exact test strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.48e-01

Only 100 of 280 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395766‑1395965
                                                                                                    |                                                                                                              
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                                               >  1:518292/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                                               >  1:2668241/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg.c                                                                                                             >  1:296623/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.ccg                                                                                                           >  1:2171429/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.ccg                                                                                                           <  1:3096234/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.ccc                                                                                                           >  1:1348793/1‑100 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTGGCCAGAGCTAGCCGCCGGGGACGTCGCTCCCCCCGCGGGGGCGAGGACCGAAAATGcg.ccc                                                                                                           >  1:2179396/1‑100 (MQ=37)
      ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                                                        >  1:2780312/1‑101 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                       <  2:2112091/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                      <  2:3052599/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                      <  1:1754342/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                    <  1:3033302/101‑1 (MQ=255)
           ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                   <  1:1674302/100‑1 (MQ=255)
           ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                   <  2:1903981/100‑1 (MQ=255)
              gggGGTGGATCGACCCCTGCGGCGTGAGTTGACAGTGATCGACGCCGTGGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  2:4317486/101‑1 (MQ=37)
              gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTTATTGACGCCTTCGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  1:3674755/101‑1 (MQ=37)
              gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  1:4056385/101‑1 (MQ=255)
              gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                >  2:2343060/1‑101 (MQ=255)
                  gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                            >  2:2664303/1‑101 (MQ=255)
                    ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                       >  1:2097416/1‑101 (MQ=255)
                     gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.g                                                                                     >  1:958105/1‑101 (MQ=255)
                      aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..a                                                                                  <  2:3415739/101‑1 (MQ=255)
                      aTCGAAACCTGCGGCGTGAGTTGCCAGCGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..a                                                                                  <  1:445703/101‑1 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcg                                                                               >  1:3610022/1‑101 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcg                                                                               >  2:2847625/1‑101 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcg                                                                               <  1:1131947/101‑1 (MQ=255)
                          aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcgc                                                                              >  2:379564/1‑101 (MQ=255)
                            aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGcgcc.g                                                                           <  1:860745/101‑1 (MQ=255)
                               tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.gcg.c                                                                       >  1:1855870/1‑101 (MQ=255)
                               tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.gcg.c                                                                       >  2:4130927/1‑101 (MQ=255)
                                 cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.g                                                                    <  1:73096/101‑1 (MQ=255)
                                  ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.Gt                                                                   <  1:116366/101‑1 (MQ=255)
                                  ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.Gt                                                                   <  2:2244434/101‑1 (MQ=255)
                                   gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTc                                                                  <  1:3225535/101‑1 (MQ=255)
                                   gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTc                                                                  <  2:1133764/101‑1 (MQ=255)
                                    cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCg                                                                 >  2:1446770/1‑101 (MQ=255)
                                     gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.T..AGCGCC.GCG.CA.GTccc                                                                >  2:3478500/1‑99 (MQ=255)
                                     gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGc                                                                <  2:3815966/101‑1 (MQ=255)
                                     gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGc                                                                <  2:1702811/101‑1 (MQ=255)
                                      tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCt                                                               <  1:3314546/101‑1 (MQ=255)
                                       gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTc                                                              >  2:3878934/1‑101 (MQ=255)
                                           tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTccccc                                                          <  1:4399951/101‑1 (MQ=255)
                                            gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTcccccc                                                         <  2:4104911/101‑1 (MQ=255)
                                             ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCcg                                                        >  1:2870650/1‑101 (MQ=255)
                                                    tAGACGCGGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCcgcgccggc                                                 <  2:3768741/101‑6 (MQ=255)
                                                     aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                <  2:351363/101‑1 (MQ=255)
                                                      gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                               >  1:2582690/1‑101 (MQ=255)
                                                        gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                             >  2:563152/11‑101 (MQ=9)
                                                        ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCg                                                <  2:108051/92‑1 (MQ=2)
                                                         ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:2967451/10‑101 (MQ=9)
                                                          ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                              <  1:191795/94‑1 (MQ=2)
                                                          ccGTGGACGCCGCTCCCCCCGCGGGGGCGTAGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgggt                                           >  1:3967150/1‑99 (MQ=255)
                                                           gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                             >  1:4188640/7‑101 (MQ=2)
                                                            gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:4171661/1‑101 (MQ=255)
                                                            gTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCgaccaccg                                         <  2:3094675/101‑8 (MQ=255)
                                                             cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:3752480/96‑1 (MQ=9)
                                                              ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:398386/97‑1 (MQ=9)
                                                               tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:946545/98‑1 (MQ=9)
                                                               ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:2555231/99‑1 (MQ=2)
                                                               gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:3000959/101‑1 (MQ=255)
                                                                ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC.G..AGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:1374008/100‑1 (MQ=2)
                                                                aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  1:2847832/101‑1 (MQ=255)
                                                                 tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                    >  1:3175568/2‑100 (MQ=9)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                   <  2:1547513/101‑1 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                  >  1:4003535/1‑101 (MQ=255)
                                                                   tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                  <  2:3159911/101‑1 (MQ=9)
                                                                   tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                  >  2:2711793/1‑101 (MQ=9)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                 <  2:3327359/101‑1 (MQ=9)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                 >  1:3476528/1‑101 (MQ=255)
                                                                    cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGG...TCTGCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                    <  2:2297876/101‑2 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                <  2:710377/101‑1 (MQ=255)
                                                                     gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGGCTGGATCGAAACAATGc                                >  2:4060624/1‑101 (MQ=9)
                                                                     gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                >  1:2245784/1‑101 (MQ=9)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                               >  1:640151/1‑101 (MQ=255)
                                                                       tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                              >  2:1848907/4‑100 (MQ=9)
                                                                       tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                              >  1:1013085/4‑100 (MQ=9)
                                                                          ccccGCGGGGGCGTGGATCGCAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                           <  2:1311323/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                           <  2:818289/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                           >  1:1523176/1‑101 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                          >  1:1269005/1‑96 (MQ=9)
                                                                           cccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                          <  2:2376764/101‑6 (MQ=9)
                                                                           cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                          >  1:1953648/1‑101 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                            >  2:3681545/1‑92 (MQ=2)
                                                                             cgggggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                        <  1:2455606/98‑1 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                        >  1:2742254/1‑94 (MQ=2)
                                                                             cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                        <  1:2921990/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                       <  1:2605373/101‑9 (MQ=2)
                                                                               cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                      >  2:805355/1‑101 (MQ=255)
                                                                                gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                     >  2:2585806/1‑91 (MQ=2)
                                                                                gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgcgggcct                     >  2:4227241/1‑91 (MQ=2)
                                                                                gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                     >  1:2410882/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   >  2:123923/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                   <  1:1103834/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                  >  2:911430/1‑101 (MQ=255)
                                                                                    gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                 <  2:4408423/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg               >  1:803500/1‑101 (MQ=255)
                                                                                       tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg              >  2:2889806/1‑101 (MQ=255)
                                                                                            cGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt         >  1:1407711/1‑101 (MQ=255)
                                                                                                  tGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg   <  2:2790534/101‑1 (MQ=255)
                                                                                                  tGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGACTg   >  1:2085057/1‑101 (MQ=255)
                                                                                                    |                                                                                                              
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395766‑1395965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG