Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,871 C→G 20.4% intergenic (+683/‑248) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8710C→G20.4% 140.9 ‑1.5 ‑0.2263intergenic (+683/‑248)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/24):  ref base (96/113):  total (126/137)
Fisher's exact test strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.66e-01

Only 100 of 283 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395772‑1395971
                                                                                                    |                                                                                                              
ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                                                              >  1:2694950/1‑101 (MQ=255)
ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCg.ccgc.c                                                                                                              >  1:2780312/1‑101 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                             <  2:2112091/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                            <  2:1057194/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                            <  1:1754342/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                          <  1:3989417/101‑1 (MQ=255)
     ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                         <  1:1674302/100‑1 (MQ=255)
     ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                                                         <  2:1903981/100‑1 (MQ=255)
        gggGGTGGATCGACCCCTGCGGCGTGAGTTGACAGTGATCGACGCCGTGGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                      <  2:4317486/101‑1 (MQ=37)
           cGTGGATCGAAGCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                   <  2:3215447/101‑1 (MQ=255)
           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                                   <  1:203238/101‑1 (MQ=255)
              ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                             >  1:2097416/1‑101 (MQ=255)
                aTCGAAACCTGCGGCGTGAGTTGCCAGCGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..a                                                                                        <  1:445703/101‑1 (MQ=255)
                 tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Ag                                                                                       <  2:1824931/101‑1 (MQ=255)
                  cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agc                                                                                      >  1:2179254/1‑101 (MQ=255)
                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcg                                                                                     <  1:1131947/101‑1 (MQ=255)
                    aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..Agcgc                                                                                    >  1:3010933/1‑101 (MQ=255)
                           cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.g                                                                          <  2:4084012/101‑1 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.Gt                                                                         <  1:116366/101‑1 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.Gt                                                                         >  1:738949/1‑101 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.Gt                                                                         <  2:2244434/101‑1 (MQ=255)
                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCg                                                                       >  2:1446770/1‑101 (MQ=255)
                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCg                                                                       >  2:1698917/1‑101 (MQ=255)
                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCg                                                                       <  2:3746749/101‑1 (MQ=255)
                                tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGcc                                                                     >  1:818371/1‑100 (MQ=255)
                                 gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTc                                                                    >  2:3878934/1‑101 (MQ=255)
                                   gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTccc                                                                  >  1:871809/1‑101 (MQ=255)
                                     tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTccccc                                                                <  1:4399951/101‑1 (MQ=255)
                                         aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCcgcg                                                            <  1:4096044/101‑1 (MQ=255)
                                          gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCgg                                                           <  2:2606637/101‑1 (MQ=255)
                                               aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                      <  2:351363/101‑1 (MQ=255)
                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:551929/101‑1 (MQ=255)
                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:3700592/101‑1 (MQ=255)
                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  2:2948441/94‑1 (MQ=2)
                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:191795/94‑1 (MQ=2)
                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:947105/94‑1 (MQ=2)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 >  1:2985572/9‑101 (MQ=9)
                                                     gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                   >  1:4188640/7‑101 (MQ=2)
                                                     aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGCCGCTCCCCCCGCGGGGGCGTGGATc                                                >  2:3786151/8‑101 (MQ=9)
                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  2:4171661/1‑101 (MQ=255)
                                                      gTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCgaccaccg                                               <  2:3094675/101‑8 (MQ=255)
                                                       cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  1:2937982/96‑1 (MQ=9)
                                                       ccggcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:2885878/97‑1 (MQ=2)
                                                        ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                             >  2:3153033/5‑99 (MQ=9)
                                                        ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:398386/97‑1 (MQ=9)
                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:4130796/99‑1 (MQ=2)
                                                         gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:3453086/101‑1 (MQ=255)
                                                          gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  2:2832938/2‑101 (MQ=2)
                                                          ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TC.G..AGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:1374008/100‑1 (MQ=2)
                                                          aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:2847832/101‑1 (MQ=255)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                         <  2:1547513/101‑1 (MQ=255)
                                                             tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                        <  2:1115217/101‑1 (MQ=9)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                       >  1:3476528/1‑101 (MQ=255)
                                                              cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGG...TCTGCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          <  2:2297876/101‑2 (MQ=2)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                      >  2:3363969/1‑101 (MQ=255)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                      <  2:710377/101‑1 (MQ=255)
                                                               gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGGCTGGATCGAAACAATGc                                      >  2:4060624/1‑101 (MQ=9)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                     >  2:2782847/1‑101 (MQ=255)
                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                     >  1:1513585/1‑101 (MQ=9)
                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                     <  2:1171247/101‑1 (MQ=9)
                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                     <  2:488466/101‑1 (MQ=9)
                                                                 tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                    >  1:1013085/4‑100 (MQ=9)
                                                                    ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                 <  2:818289/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                <  2:2376764/101‑6 (MQ=9)
                                                                      ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTC.T..GGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                  >  2:3681545/1‑92 (MQ=2)
                                                                       cgggggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                              <  1:2455606/98‑1 (MQ=255)
                                                                       cgcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                              >  1:2742254/1‑94 (MQ=2)
                                                                        gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggc                             >  2:3028904/1‑93 (MQ=2)
                                                                        gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                             <  1:2605373/101‑9 (MQ=2)
                                                                         cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                            <  1:320679/101‑10 (MQ=2)
                                                                         cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                            >  2:805355/1‑101 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                            <  2:2694760/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TG.G..GGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgcgggcct                           >  2:4227241/1‑91 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                           >  1:2410882/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                         <  2:1275696/101‑1 (MQ=255)
                                                                            gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                         <  1:1103834/101‑1 (MQ=255)
                                                                             ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                        >  2:911430/1‑101 (MQ=255)
                                                                              gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                       <  2:4408423/101‑1 (MQ=255)
                                                                                gTGTATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                     <  2:2305763/101‑1 (MQ=255)
                                                                                 tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                    <  1:3760600/101‑1 (MQ=255)
                                                                                  ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                   >  1:686999/1‑101 (MQ=255)
                                                                                  ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                   <  1:4035720/101‑1 (MQ=255)
                                                                                   gATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                  >  1:3282650/1‑101 (MQ=255)
                                                                                      cGAACCTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               <  2:3813901/101‑1 (MQ=255)
                                                                                      cGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               >  1:1407711/1‑101 (MQ=255)
                                                                                      cGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               >  1:363687/1‑101 (MQ=255)
                                                                                      cGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               <  1:3368731/101‑1 (MQ=255)
                                                                                      cGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt               <  1:308008/101‑1 (MQ=255)
                                                                                       gAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCC.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg              >  1:1347094/1‑101 (MQ=255)
                                                                                        aaaCTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg             >  1:1253006/1‑101 (MQ=255)
                                                                                           cTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt          <  2:2850602/101‑1 (MQ=255)
                                                                                             gcg.cCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg        <  2:2582519/101‑1 (MQ=255)
                                                                                              gcaccgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg        >  1:2563201/4‑101 (MQ=255)
                                                                                               g.ccgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGGGTTCATGGCCTACCTCGGGCAGTGGACTGGTc      >  2:2446176/1‑101 (MQ=255)
                                                                                               g.ccgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc      <  1:1950262/101‑1 (MQ=255)
                                                                                               g.ccgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc      <  1:1798864/101‑1 (MQ=255)
                                                                                                 ccgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg     >  1:3171844/1‑101 (MQ=255)
                                                                                                  cgc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc    >  1:2222405/1‑101 (MQ=255)
                                                                                                   gc.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCCTGGCCTACGTCGGGCAGCGGACTGGGCGCt   >  1:2297049/1‑101 (MQ=255)
                                                                                                    c.cTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc  <  1:188158/101‑1 (MQ=255)
                                                                                                    |                                                                                                              
CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TC.G..AGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTC  >  NC_014034/1395772‑1395971

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG