BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,872	C→T	22.0%	intergenic (+684/‑247)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,872	0	C→T	22.0%	151.7 ‑1.0 ‑0.0	262	intergenic (+684/‑247)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (30/24): ref base (89/99): total (125/137)
Fisher's exact test strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 284 total aligned reads displayed.


CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCC  >  NC_014034/1395772‑1395972


ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCgccgcc                                                                                                             >  1:2694950/1‑101 (MQ=255)


 tccgatctggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                                                                            <  1:4222223/93‑1 (MQ=255)


  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                           <  2:1057194/101‑1 (MQ=255)


  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                           <  2:3052599/101‑1 (MQ=255)


  ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                           <  1:1754342/101‑1 (MQ=255)


   cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                          <  2:1208097/101‑1 (MQ=255)


    gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                                                                         <  1:3989417/101‑1 (MQ=255)


     ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                                                                                                        <  1:1674302/100‑1 (MQ=255)


      gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                                                                                                       >  2:4362983/1‑101 (MQ=255)


        gggGGTGGATCGACCCCTGCGGCGTGAGTTGACAGTGATCGACGCCGTGGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                     <  2:4317486/101‑1 (MQ=37)


        gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                     >  2:2343060/1‑101 (MQ=255)


          gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                   <  1:280238/101‑1 (MQ=255)


           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                  >  1:3100982/1‑101 (MQ=255)


           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                  <  1:829145/101‑1 (MQ=255)


           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                  <  1:203238/101‑1 (MQ=255)


              ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...Tc                                                                                            >  1:2097416/1‑101 (MQ=255)


                aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..a                                                                                       <  2:3415739/101‑1 (MQ=255)


                aTCGAAACCTGCGGCGTGAGTTGCCAGCGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..a                                                                                       <  1:445703/101‑1 (MQ=255)


                  cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agc                                                                                     >  1:2179254/1‑101 (MQ=255)


                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                    <  1:1131947/101‑1 (MQ=255)


                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                    <  2:3951446/101‑1 (MQ=255)


                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                    >  2:2847625/1‑101 (MQ=255)


                    aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcgc                                                                                   >  2:379564/1‑101 (MQ=255)


                           cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.g                                                                           <  2:4084012/101‑1 (MQ=255)


                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                          <  1:116366/101‑1 (MQ=255)


                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                          <  2:2244434/101‑1 (MQ=255)


                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                        <  2:2380393/101‑1 (MQ=255)


                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                        >  2:1446770/1‑101 (MQ=255)


                               gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.T..AGCGCCGCGCA.GTccc                                                                       >  2:3478500/1‑99 (MQ=255)


                               gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                       <  2:3815966/101‑1 (MQ=255)


                               gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                       <  2:1702811/101‑1 (MQ=255)


                                  aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcc                                                                    >  2:4035463/1‑101 (MQ=255)


                                     tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTccccc                                                                 <  1:4399951/101‑1 (MQ=255)


                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcccccc                                                                >  2:1388028/1‑101 (MQ=255)


                                       ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcg                                                               >  1:2757081/1‑101 (MQ=255)


                                         aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcg                                                             >  1:886443/1‑101 (MQ=255)


                                          gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCgg                                                            <  2:2606637/101‑1 (MQ=255)


                                            gATAGACTCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCgggg                                                          <  2:217129/101‑1 (MQ=255)


                                              tAGACGCGGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcgccggc                                                        <  2:3768741/101‑6 (MQ=255)


                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                     <  1:3700592/101‑1 (MQ=255)


                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                     <  1:551929/101‑1 (MQ=255)


                                                   ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  2:2967451/10‑101 (MQ=9)


                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                     <  1:947105/94‑1 (MQ=2)


                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  >  1:2985572/9‑101 (MQ=9)


                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  2:1944318/1‑101 (MQ=255)


                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  2:4171661/1‑101 (MQ=255)


                                                      gTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCgaccaccg                                                <  2:3094675/101‑8 (MQ=255)


                                                       cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               <  1:2937982/96‑1 (MQ=9)


                                                       ccggcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:2885878/97‑1 (MQ=2)


                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  2:4130796/99‑1 (MQ=2)


                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  2:2555231/99‑1 (MQ=2)


                                                         gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             >  2:3989733/1‑101 (MQ=255)


                                                          atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            >  2:3545597/3‑101 (MQ=9)


                                                          aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  1:2847832/101‑1 (MQ=255)


                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                          <  2:1547513/101‑1 (MQ=255)


                                                             tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                         <  2:3159911/101‑1 (MQ=9)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                        <  2:3327359/101‑1 (MQ=9)


                                                              cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                        <  1:3603408/101‑1 (MQ=9)


                                                              cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGG...TCTGCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                           <  2:2297876/101‑2 (MQ=2)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                       <  2:710377/101‑1 (MQ=255)


                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                      >  1:1513585/1‑101 (MQ=9)


                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                      <  2:488466/101‑1 (MQ=9)


                                                                cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                      <  1:3658021/101‑1 (MQ=9)


                                                                 tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                     >  2:1848907/4‑100 (MQ=9)


                                                                 tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                     >  1:1013085/4‑100 (MQ=9)


                                                                    ccccGCGGGGGCGTGGATCGCAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                  <  2:1311323/101‑1 (MQ=255)


                                                                    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                  >  1:792259/1‑101 (MQ=255)


                                                                    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                  <  2:818289/101‑1 (MQ=255)


                                                                      ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                   >  2:3681545/1‑92 (MQ=2)


                                                                       cgggggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                               <  1:2455606/98‑1 (MQ=255)


                                                                        gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                              <  1:2605373/101‑9 (MQ=2)


                                                                         cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                             <  1:320679/101‑10 (MQ=2)


                                                                         cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                             <  2:2694760/101‑1 (MQ=255)


                                                                         cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                             >  2:805355/1‑101 (MQ=255)


                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                            >  1:2387063/1‑91 (MQ=2)


                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                            >  2:2585806/1‑91 (MQ=2)


                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                            <  1:3029125/101‑11 (MQ=2)


                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgcgggcct                            >  2:4227241/1‑91 (MQ=2)


                                                                          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                            >  1:2410882/1‑101 (MQ=255)


                                                                            gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                          <  1:1103834/101‑1 (MQ=255)


                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                         >  2:911430/1‑101 (MQ=255)


                                                                                 tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                     <  1:3760600/101‑1 (MQ=255)


                                                                                 tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                     >  2:2889806/1‑101 (MQ=255)


                                                                                  ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                    >  1:686999/1‑101 (MQ=255)


                                                                                  ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                    <  1:4035720/101‑1 (MQ=255)


                                                                                   gATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                   >  1:3282650/1‑101 (MQ=255)


                                                                                      cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                <  1:3368731/101‑1 (MQ=255)


                                                                                      cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                <  1:1940615/101‑1 (MQ=255)


                                                                                       gAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCCCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg               >  1:1347094/1‑101 (MQ=255)


                                                                                        aaaCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg              >  1:1253006/1‑101 (MQ=255)


                                                                                          aCTGCGCCGCCTATCGCATCCGG...GC.G..AGCGCCACGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc            <  2:1122584/101‑1 (MQ=255)


                                                                                            tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg          <  2:2790534/101‑1 (MQ=255)


                                                                                             gcaccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg         >  1:2563201/4‑101 (MQ=255)


                                                                                               gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc       <  1:1426519/101‑1 (MQ=255)


                                                                                               gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc       <  1:1798864/101‑1 (MQ=255)


                                                                                               gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc       <  1:1950262/101‑1 (MQ=255)


                                                                                                ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg      <  1:4119824/101‑1 (MQ=255)


                                                                                                ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg      >  1:3171844/1‑101 (MQ=255)


                                                                                                 cgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc     >  1:2222405/1‑101 (MQ=255)


                                                                                                   ccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc   <  1:188158/101‑1 (MQ=255)


CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCC  >  NC_014034/1395772‑1395972

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG