BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,874	A→C	27.0%	intergenic (+686/‑245)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,874	0	A→C	27.0%	217.0 0.0 ‑0.2	273	intergenic (+686/‑245)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (36/38): ref base (96/103): total (132/141)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.03e-01

Only 100 of 284 total aligned reads displayed.


CCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCC  >  NC_014034/1395774‑1395974


ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                             <  2:3052599/101‑1 (MQ=255)


ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                             <  1:1754342/101‑1 (MQ=255)


  gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                                                                           <  1:3989417/101‑1 (MQ=255)


   ggggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                                                                                                          <  1:1674302/100‑1 (MQ=255)


    gggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                                                                                                         >  2:4362983/1‑101 (MQ=255)


      gggGGTGGATCGACCCCTGCGGCGTGAGTTGACAGTGATCGACGCCGTGGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                       <  2:4317486/101‑1 (MQ=37)


      gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                       >  2:2343060/1‑101 (MQ=255)


        gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                     <  1:280238/101‑1 (MQ=255)


        gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                     >  2:4119564/1‑101 (MQ=255)


         cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                    >  1:2096983/1‑101 (MQ=255)


         cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                    >  1:3100982/1‑101 (MQ=255)


           tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...t                                                                                               >  1:2436354/1‑101 (MQ=255)


             gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.g                                                                                            >  1:958105/1‑101 (MQ=255)


              aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..a                                                                                         <  2:3415739/101‑1 (MQ=255)


                 gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                      <  1:1131947/101‑1 (MQ=255)


                 gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                      >  2:2847625/1‑101 (MQ=255)


                    aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGcgccg                                                                                   <  1:860745/101‑1 (MQ=255)


                         cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.g                                                                             <  1:73096/101‑1 (MQ=255)


                          ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                            <  2:2244434/101‑1 (MQ=255)


                          ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                            <  1:116366/101‑1 (MQ=255)


                           gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTc                                                                           <  2:1133764/101‑1 (MQ=255)


                            cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                          <  2:2380393/101‑1 (MQ=255)


                            cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                          >  2:1479833/1‑101 (MQ=255)


                            cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                          <  2:3746749/101‑1 (MQ=255)


                             gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.T..AGCGCCGCGCA.GTccc                                                                         >  2:3478500/1‑99 (MQ=255)


                             gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                         <  2:1702811/101‑1 (MQ=255)


                               gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTc                                                                       >  2:3878934/1‑101 (MQ=255)


                                   tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTccccc                                                                   >  1:458506/1‑101 (MQ=255)


                                        gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCgg                                                              <  2:2606637/101‑1 (MQ=255)


                                         tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCggg                                                             <  1:260908/101‑1 (MQ=255)


                                          gATAGACTCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCgggg                                                            <  2:217129/101‑1 (MQ=255)


                                              gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                        >  1:2582690/1‑101 (MQ=255)


                                              gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                        >  1:2010479/1‑101 (MQ=255)


                                               aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:3700592/101‑1 (MQ=255)


                                                ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCg                                                         <  2:108051/92‑1 (MQ=2)


                                                ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCg                                                         >  1:4306988/10‑101 (MQ=2)


                                                 gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                     >  1:4417930/1‑101 (MQ=255)


                                                 ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                     >  2:2967451/10‑101 (MQ=9)


                                                  ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:191795/94‑1 (MQ=2)


                                                  caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    >  1:2985572/9‑101 (MQ=9)


                                                   aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGCCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  2:3786151/8‑101 (MQ=9)


                                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  2:1944318/1‑101 (MQ=255)


                                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  >  2:4161362/1‑101 (MQ=255)


                                                    gTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCgaccaccg                                                  <  2:3094675/101‑8 (MQ=255)


                                                     cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  1:2937982/96‑1 (MQ=9)


                                                     ccggcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:2885878/97‑1 (MQ=2)


                                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  2:398386/97‑1 (MQ=9)


                                                       tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:946545/98‑1 (MQ=9)


                                                       ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                  <  2:2555231/99‑1 (MQ=2)


                                                        atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              >  2:3545597/3‑101 (MQ=9)


                                                        aCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              <  1:2847832/101‑1 (MQ=255)


                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                            <  2:1438586/101‑1 (MQ=255)


                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                            <  2:1547513/101‑1 (MQ=255)


                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                            >  1:247030/1‑101 (MQ=255)


                                                          gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                            >  1:2214862/1‑99 (MQ=9)


                                                           tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                           <  2:3159911/101‑1 (MQ=9)


                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                          <  2:3327359/101‑1 (MQ=9)


                                                            cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                          <  1:3603408/101‑1 (MQ=9)


                                                            cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGG...TCTGCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                             <  2:2297876/101‑2 (MQ=2)


                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                         <  2:710377/101‑1 (MQ=255)


                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                        >  1:640151/1‑101 (MQ=255)


                                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                        <  1:3658021/101‑1 (MQ=9)


                                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                        >  1:2140371/1‑101 (MQ=9)


                                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                        >  1:1513585/1‑101 (MQ=9)


                                                              cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                        <  2:488466/101‑1 (MQ=9)


                                                               tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                       >  2:1848907/4‑100 (MQ=9)


                                                               tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                       >  1:1013085/4‑100 (MQ=9)


                                                                  ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                    <  2:818289/101‑1 (MQ=255)


                                                                   cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                   <  2:1348696/101‑1 (MQ=255)


                                                                    ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                     >  2:3681545/1‑92 (MQ=2)


                                                                     cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                 >  1:2742254/1‑94 (MQ=2)


                                                                       cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                               <  1:320679/101‑10 (MQ=2)


                                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                               <  2:2694760/101‑1 (MQ=255)


                                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                               >  2:805355/1‑101 (MQ=255)


                                                                        gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                              >  2:2585806/1‑91 (MQ=2)


                                                                        gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                              >  1:2387063/1‑91 (MQ=2)


                                                                        gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                              <  1:3029125/101‑11 (MQ=2)


                                                                        gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATCCggcgcgggcct                              >  2:4227241/1‑91 (MQ=2)


                                                                          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                            >  2:123923/1‑101 (MQ=255)


                                                                          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                            <  1:1103834/101‑1 (MQ=255)


                                                                           ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                           >  2:911430/1‑101 (MQ=255)


                                                                              gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                        >  1:803500/1‑101 (MQ=255)


                                                                              gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                        >  1:1945047/1‑101 (MQ=255)


                                                                               tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                       >  2:2889806/1‑101 (MQ=255)


                                                                               tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                       <  1:3760600/101‑1 (MQ=255)


                                                                                ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                      <  1:4035720/101‑1 (MQ=255)


                                                                                 gATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                     >  1:3282650/1‑101 (MQ=255)


                                                                                    cGAACCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                  <  2:3813901/101‑1 (MQ=255)


                                                                                    cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                  <  1:3368731/101‑1 (MQ=255)


                                                                                     gAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCCCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                 >  1:1347094/1‑101 (MQ=255)


                                                                                      aaaCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                >  1:1253006/1‑101 (MQ=255)


                                                                                         cTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt             >  2:4061802/1‑101 (MQ=255)


                                                                                          tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg            <  2:2790534/101‑1 (MQ=255)


                                                                                             gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc         <  1:1426519/101‑1 (MQ=255)


                                                                                             gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc         <  1:1798864/101‑1 (MQ=255)


                                                                                              ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg        <  1:4119824/101‑1 (MQ=255)


                                                                                              ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg        >  1:3171844/1‑101 (MQ=255)


                                                                                                gccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCCTGGCCTACGTCGGGCAGCGGACTGGGCGCt      >  1:2297049/1‑101 (MQ=255)


                                                                                                 ccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc     <  1:188158/101‑1 (MQ=255)


                                                                                                  cTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc    <  2:4014313/101‑1 (MQ=255)


CCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCC  >  NC_014034/1395774‑1395974

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG