BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,886	C→G	20.6%	intergenic (+698/‑233)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,886	0	C→G	20.6%	142.0 ‑1.5 ‑0.8	262	intergenic (+698/‑233)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (30/24): ref base (96/112): total (126/136)
Fisher's exact test strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.58e-01

Only 100 of 272 total aligned reads displayed.


GGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395786‑1395986


ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...Tc                                                                                                          <  1:2907708/101‑1 (MQ=255)


 gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.g                                                                                                        >  1:958105/1‑101 (MQ=255)


   tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Ag                                                                                                    <  2:1824931/101‑1 (MQ=255)


    cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agc                                                                                                   >  1:4438084/1‑101 (MQ=255)


    cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agc                                                                                                   >  1:2179254/1‑101 (MQ=255)


     gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                                  >  2:2847625/1‑101 (MQ=255)


      aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcgc                                                                                                 >  1:3010933/1‑101 (MQ=255)


      aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcgc                                                                                                 >  2:379564/1‑101 (MQ=255)


              ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                        <  2:2244434/101‑1 (MQ=255)


              ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                        >  1:738949/1‑101 (MQ=255)


               gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTc                                                                                       <  1:3225535/101‑1 (MQ=255)


               gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTc                                                                                       <  2:1133764/101‑1 (MQ=255)


                cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                      >  2:1698917/1‑101 (MQ=255)


                cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                      >  2:1479833/1‑101 (MQ=255)


                cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                      <  2:2380393/101‑1 (MQ=255)


                 gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                                     <  2:3815966/101‑1 (MQ=255)


                  tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGcc                                                                                    >  1:818371/1‑100 (MQ=255)


                   gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTc                                                                                   >  2:3878934/1‑101 (MQ=255)


                    aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcc                                                                                  >  2:4035463/1‑101 (MQ=255)


                       tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTccccc                                                                               <  1:4399951/101‑1 (MQ=255)


                        gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcccccc                                                                              >  2:1388028/1‑101 (MQ=255)


                        gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcccccc                                                                              <  2:4104911/101‑1 (MQ=255)


                         ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcg                                                                             >  1:2870650/1‑101 (MQ=255)


                          cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgc                                                                            <  1:4181534/101‑1 (MQ=255)


                           aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcg                                                                           >  1:886443/1‑101 (MQ=255)


                           aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcg                                                                           <  1:4096044/101‑1 (MQ=255)


                             tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCggg                                                                         <  1:260908/101‑1 (MQ=255)


                                tAGACGCGGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcgccggc                                                                      <  2:3768741/101‑6 (MQ=255)


                                  gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                    >  1:2010479/1‑101 (MQ=255)


                                   aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:2403513/101‑1 (MQ=255)


                                    gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  2:3486204/11‑101 (MQ=9)


                                    ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCg                                                                     <  2:108051/92‑1 (MQ=2)


                                     cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGt                                                                    >  2:4322568/9‑101 (MQ=2)


                                     ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  2:2967451/10‑101 (MQ=9)


                                      ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:191795/94‑1 (MQ=2)


                                      ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:2816724/94‑1 (MQ=2)


                                      caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  1:2985572/9‑101 (MQ=9)


                                       aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:1730803/94‑1 (MQ=9)


                                        gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:4161362/1‑101 (MQ=255)


                                        gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:1446877/101‑1 (MQ=255)


                                        gTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCgaccaccg                                                              <  2:3094675/101‑8 (MQ=255)


                                        gTGGACGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCAGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  1:3853260/1‑101 (MQ=255)


                                         tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  1:3138718/1‑101 (MQ=255)


                                          ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                                            >  2:3153033/5‑99 (MQ=9)


                                          ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  2:398386/97‑1 (MQ=9)


                                           tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  1:1374105/4‑101 (MQ=9)


                                           tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:2530999/98‑1 (MQ=9)


                                           tacctccccccccccGCGGAAGCGTAANTCNAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:2452505/92‑1 (MQ=25)


                                           ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:3425650/99‑1 (MQ=2)


                                              ggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                        <  1:2137740/99‑1 (MQ=255)


                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                        <  2:1547513/101‑1 (MQ=255)


                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                       >  1:4003535/1‑101 (MQ=255)


                                               tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                       <  1:2604376/101‑1 (MQ=9)


                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                      <  2:3327359/101‑1 (MQ=9)


                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                      <  2:2668069/101‑1 (MQ=255)


                                                cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                      <  1:3603408/101‑1 (MQ=9)


                                                cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGG...TCTGCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                         <  2:2297876/101‑2 (MQ=2)


                                                 gCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcactgc                                                     >  1:1579219/1‑96 (MQ=9)


                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                    >  2:2782847/1‑101 (MQ=255)


                                                   tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                   >  1:1013085/4‑100 (MQ=9)


                                                   tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                   >  1:1886760/4‑100 (MQ=9)


                                                   tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                   >  1:3704563/1‑101 (MQ=255)


                                                      ccccGCGGGGGCGTGGATCGCAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                <  2:1311323/101‑1 (MQ=255)


                                                      ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                <  2:818289/101‑1 (MQ=255)


                                                       cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                               >  1:1269005/1‑96 (MQ=9)


                                                       cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                               >  2:777421/1‑101 (MQ=255)


                                                         cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                             >  1:2742254/1‑94 (MQ=2)


                                                          gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                            >  2:2595820/1‑93 (MQ=2)


                                                           cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                           >  2:805355/1‑101 (MQ=255)


                                                           cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                           >  2:569951/1‑101 (MQ=255)


                                                            gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                          <  1:2941047/101‑11 (MQ=2)


                                                            gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                          <  2:3610007/101‑11 (MQ=2)


                                                              gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                        <  1:1103834/101‑1 (MQ=255)


                                                              gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                        <  1:1245223/101‑1 (MQ=255)


                                                               ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                       >  2:911430/1‑101 (MQ=255)


                                                                  gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                    >  2:3723964/1‑101 (MQ=255)


                                                                   tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                   >  2:4306281/1‑101 (MQ=255)


                                                                   tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                   >  2:2889806/1‑101 (MQ=255)


                                                                    ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                  >  1:686999/1‑101 (MQ=255)


                                                                     gATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                 >  1:3282650/1‑101 (MQ=255)


                                                                        cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                              >  1:363687/1‑101 (MQ=255)


                                                                        cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                              <  1:3368731/101‑1 (MQ=255)


                                                                              tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                        <  1:1146497/101‑1 (MQ=255)


                                                                               gcgcCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                       <  2:2582519/101‑1 (MQ=255)


                                                                                 gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGGGTTCATGGCCTACCTCGGGCAGTGGACTGGTc                     >  2:2446176/1‑101 (MQ=255)


                                                                                 gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                     <  1:1950262/101‑1 (MQ=255)


                                                                                 gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                     <  1:1806193/101‑1 (MQ=255)


                                                                                   cgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc                   >  1:2222405/1‑101 (MQ=255)


                                                                                       tATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGTGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc               >  2:2046569/1‑101 (MQ=255)


                                                                                       tATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc               >  1:234149/1‑101 (MQ=255)


                                                                                          cGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc            >  2:282878/1‑101 (MQ=255)


                                                                                           gCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg           <  2:1944918/101‑1 (MQ=255)


                                                                                           gCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGCTCGCTCCCCCcg           <  1:3187453/101‑1 (MQ=255)


                                                                                           ccATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg           <  2:4437812/100‑1 (MQ=255)


                                                                                               ccGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg       >  2:1261852/1‑101 (MQ=255)


                                                                                               ccGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg       <  1:497600/101‑1 (MQ=255)


                                                                                                  g...TC.G..AGCGCCGCGCA.GTCGCGCCCCCCGCGGTGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc    <  2:4070673/101‑1 (MQ=255)


                                                                                                      tC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTACATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg   >  2:2366195/1‑101 (MQ=255)


                                                                                                       c.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg  >  1:569194/1‑100 (MQ=255)


                                                                                                       c.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg  >  2:3374230/1‑100 (MQ=255)


GGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395786‑1395986

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG