Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,896 G→T 7.7% intergenic (+708/‑223) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8960G→T 7.7% 38.1 ‑2.2 0.0255intergenic (+708/‑223)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/14):  ref base (116/118):  total (123/132)
Fisher's exact test strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395797‑1395996
                                                                                                      |                                                                                                     
tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCgcgc                                                                                                      >  1:1855870/1‑101 (MQ=255)
  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                                   <  1:1787065/101‑1 (MQ=255)
  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                                   <  1:73096/101‑1 (MQ=255)
   ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.Gt                                                                                                  <  1:116366/101‑1 (MQ=255)
     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                                <  2:3746749/101‑1 (MQ=255)
     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                                >  2:1446770/1‑101 (MQ=255)
     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                                <  2:2380393/101‑1 (MQ=255)
      gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGc                                                                                               >  2:3779583/1‑101 (MQ=255)
        gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTc                                                                                             >  2:3878934/1‑101 (MQ=255)
         aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                                                            >  2:4035463/1‑101 (MQ=255)
          gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccc                                                                                           >  1:871809/1‑101 (MQ=255)
            tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccccc                                                                                         <  1:4399951/101‑1 (MQ=255)
             gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                                                        <  2:4104911/101‑1 (MQ=255)
             gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                                                        >  2:1388028/1‑101 (MQ=255)
              ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcg                                                                                       >  1:2870650/1‑101 (MQ=255)
               cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgc                                                                                      <  1:4181534/101‑1 (MQ=255)
                  tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCCCggg                                                                                   >  2:2208912/1‑101 (MQ=255)
                      aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCg                                                                               <  2:351363/101‑1 (MQ=255)
                       gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                              <  2:2436197/101‑1 (MQ=255)
                       gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                              >  1:2010479/1‑101 (MQ=255)
                        aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:2403513/101‑1 (MQ=255)
                        aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:551929/101‑1 (MQ=255)
                         gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                                                            <  2:1529634/91‑1 (MQ=9)
                          gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGGGGa                                                                           >  1:1275789/1‑101 (MQ=255)
                          cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGt                                                                              >  2:4322568/9‑101 (MQ=2)
                          ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           >  2:2967451/10‑101 (MQ=9)
                           ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:947105/94‑1 (MQ=2)
                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                         >  2:3835329/8‑101 (MQ=9)
                             gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  2:4161362/1‑101 (MQ=255)
                             gTGGACGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCAGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        >  1:3853260/1‑101 (MQ=255)
                             cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  2:2286306/96‑1 (MQ=0)
                               ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  2:398386/97‑1 (MQ=9)
                                ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  2:2555231/99‑1 (MQ=2)
                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:3000959/101‑1 (MQ=255)
                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  2:3453086/101‑1 (MQ=255)
                                  tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                   >  1:3175568/2‑100 (MQ=9)
                                   gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                                  >  1:2214862/1‑99 (MQ=9)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                 >  1:4003535/1‑101 (MQ=255)
                                    tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                                 <  2:1115217/101‑1 (MQ=9)
                                    tCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                 <  2:3100758/101‑1 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                >  1:3476528/1‑101 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                <  2:2668069/101‑1 (MQ=255)
                                      gccccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                               <  1:1691305/98‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                              >  2:2782847/1‑101 (MQ=255)
                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                              <  1:3658021/101‑1 (MQ=9)
                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                              >  1:2140371/1‑101 (MQ=9)
                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                              <  2:1171247/101‑1 (MQ=9)
                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                              >  1:1513585/1‑101 (MQ=9)
                                        tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                             >  2:1848907/4‑100 (MQ=9)
                                        tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                             >  1:676728/4‑100 (MQ=9)
                                        tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                             >  1:1013085/4‑100 (MQ=9)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                             >  1:3704563/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                          <  2:818289/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                         >  2:777421/1‑101 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                         >  1:1953648/1‑101 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                                           >  2:3681545/1‑92 (MQ=2)
                                              cgggggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                       <  1:2455606/98‑1 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                                       >  1:2742254/1‑94 (MQ=2)
                                              cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                       <  1:2921990/101‑1 (MQ=255)
                                              cgcgGGGGCGTGGACCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                       <  2:2941580/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggc                                                      >  2:3028904/1‑93 (MQ=2)
                                               gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                      >  2:2595820/1‑93 (MQ=2)
                                               gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                      <  1:2605373/101‑9 (MQ=2)
                                                cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                     <  1:320679/101‑10 (MQ=2)
                                                cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                     >  2:569951/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                    <  1:2664475/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                    >  1:2410882/1‑101 (MQ=255)
                                                   gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                  <  1:1103834/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                 >  2:911430/1‑101 (MQ=255)
                                                       gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                              >  1:803500/1‑101 (MQ=255)
                                                        tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                             >  2:4306281/1‑101 (MQ=255)
                                                          gATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                           >  1:3282650/1‑101 (MQ=255)
                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                        <  1:308008/101‑1 (MQ=255)
                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                        >  1:1407711/1‑101 (MQ=255)
                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                        <  1:1940615/101‑1 (MQ=255)
                                                               aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                      <  2:2849441/101‑1 (MQ=255)
                                                               aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                      >  1:1253006/1‑101 (MQ=255)
                                                                aaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                     <  2:3205327/101‑1 (MQ=255)
                                                                    gcgcCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                 <  2:2582519/101‑1 (MQ=255)
                                                                    gcaccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                 >  1:2563201/4‑101 (MQ=255)
                                                                      gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                               >  1:3359068/1‑101 (MQ=255)
                                                                       ccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                              <  1:4119824/101‑1 (MQ=255)
                                                                         gccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCCTGGCCTACGTCGGGCAGCGGACTGGGCGCt                            >  1:2297049/1‑101 (MQ=255)
                                                                            tATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGTGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                         >  2:2046569/1‑101 (MQ=255)
                                                                             aTCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                        >  1:1900248/1‑101 (MQ=255)
                                                                               cGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                      <  1:2970611/101‑1 (MQ=255)
                                                                               cGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                      >  2:282878/1‑101 (MQ=255)
                                                                                gCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                     <  2:1944918/101‑1 (MQ=255)
                                                                                ccATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                     <  2:4437812/100‑1 (MQ=255)
                                                                                    ccGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                 >  2:1261852/1‑101 (MQ=255)
                                                                                    ccGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                 <  1:497600/101‑1 (MQ=255)
                                                                                     cGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                <  2:234122/101‑1 (MQ=255)
                                                                                       g...TCGAGCGCCGCGCA.GTCGCGCCCCCCGCGGTGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc              <  2:4070673/101‑1 (MQ=255)
                                                                                            cGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg            >  2:3374230/1‑100 (MQ=255)
                                                                                                cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGAt        >  2:1590873/1‑101 (MQ=255)
                                                                                                    gggcA.GGCGCTCCCCGCGCGGGGGCGTGGACCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  1:2800031/99‑1 (MQ=255)
                                                                                                    cggcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  2:1320678/3‑101 (MQ=255)
                                                                                                      gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  2:1766417/101‑1 (MQ=255)
                                                                                                      gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  2:80239/101‑1 (MQ=255)
                                                                                                      gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  2:3808864/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
TGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395797‑1395996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG