BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,897	C→G	8.6%	intergenic (+709/‑222)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,897	0	C→G	8.6%	46.3 ‑2.1 ‑0.6	255	intergenic (+709/‑222)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (7/15): ref base (116/117): total (123/132)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.31e-01

Only 100 of 273 total aligned reads displayed.


TGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1395797‑1395997


tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCgcgc                                                                                                       >  1:1855870/1‑101 (MQ=255)


  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.g                                                                                                    <  1:1787065/101‑1 (MQ=255)


  cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.g                                                                                                    <  1:73096/101‑1 (MQ=255)


   ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                                   <  1:116366/101‑1 (MQ=255)


   ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                                   <  2:2244434/101‑1 (MQ=255)


     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                                 <  2:3746749/101‑1 (MQ=255)


     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                                 <  2:2380393/101‑1 (MQ=255)


     cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                                 >  2:1479833/1‑101 (MQ=255)


      gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.T..AGCGCCGCGCA.GTccc                                                                                                >  2:3478500/1‑99 (MQ=255)


      gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                                                >  1:971384/1‑101 (MQ=255)


            tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGGCGCGCA.GTCGCCcctcc                                                                                          >  2:4308313/1‑101 (MQ=255)


            tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTccccc                                                                                          <  1:4399951/101‑1 (MQ=255)


             gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcccccc                                                                                         >  2:1388028/1‑101 (MQ=255)


              ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcg                                                                                        >  1:2870650/1‑101 (MQ=255)


                aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcg                                                                                      >  1:886443/1‑101 (MQ=255)


                       gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                               >  1:2010479/1‑101 (MQ=255)


                        aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                              <  1:2403513/101‑1 (MQ=255)


                          gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGGGGa                                                                            >  1:1275789/1‑101 (MQ=255)


                           ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                              <  2:2948441/94‑1 (MQ=2)


                           ccGTGGACGCCGCTCCCCCCGCGGGGGCGTAGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgggt                                                                           >  1:3967150/1‑99 (MQ=255)


                            gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                             >  1:4188640/7‑101 (MQ=2)


                            aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGCCGCTCCCCCCGCGGGGGCGTGGATc                                                                          >  2:3786151/8‑101 (MQ=9)


                             gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         >  2:4171661/1‑101 (MQ=255)


                              tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        >  1:3138718/1‑101 (MQ=255)


                              cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                        <  1:3752480/96‑1 (MQ=9)


                               ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                                                       >  2:3153033/5‑99 (MQ=9)


                                ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  2:4130796/99‑1 (MQ=2)


                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      <  1:3000959/101‑1 (MQ=255)


                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      >  2:3989733/1‑101 (MQ=255)


                                 atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                     >  2:3545597/3‑101 (MQ=9)


                                  tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                    >  1:3175568/2‑100 (MQ=9)


                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                   <  2:1547513/101‑1 (MQ=255)


                                   gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                                   >  1:2214862/1‑99 (MQ=9)


                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                  >  1:4003535/1‑101 (MQ=255)


                                    tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                                  <  2:1115217/101‑1 (MQ=9)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                                 <  2:3327359/101‑1 (MQ=9)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                 >  1:3476528/1‑101 (MQ=255)


                                     cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGG...TCTGCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                    <  2:2297876/101‑2 (MQ=2)


                                      gccccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                                <  1:1691305/98‑1 (MQ=255)


                                      gCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGGCTGGATCGAAACAATGc                                                                >  2:4060624/1‑101 (MQ=9)


                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                               <  1:3658021/101‑1 (MQ=9)


                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                               <  2:1171247/101‑1 (MQ=9)


                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                               >  1:1513585/1‑101 (MQ=9)


                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                               >  1:2140371/1‑101 (MQ=9)


                                       cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                               <  2:488466/101‑1 (MQ=9)


                                        tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                              >  1:1013085/4‑100 (MQ=9)


                                           ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                           <  2:818289/101‑1 (MQ=255)


                                            cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                          >  2:777421/1‑101 (MQ=255)


                                            cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                          >  1:1953648/1‑101 (MQ=255)


                                              cgggggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                        <  1:2455606/98‑1 (MQ=255)


                                              cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                                        >  1:2742254/1‑94 (MQ=2)


                                              cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                        <  1:2921990/101‑1 (MQ=255)


                                               gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggc                                                       >  2:3028904/1‑93 (MQ=2)


                                               gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                       <  1:2605373/101‑9 (MQ=2)


                                                cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                      <  1:320679/101‑10 (MQ=2)


                                                cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                      <  2:2694760/101‑1 (MQ=255)


                                                   gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                   <  2:1275696/101‑1 (MQ=255)


                                                   gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                   <  1:1103834/101‑1 (MQ=255)


                                                    ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                  >  2:911430/1‑101 (MQ=255)


                                                     gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                 <  2:4408423/101‑1 (MQ=255)


                                                       gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                               >  1:1945047/1‑101 (MQ=255)


                                                       gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                               >  1:803500/1‑101 (MQ=255)


                                                         ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                             >  1:686999/1‑101 (MQ=255)


                                                          gATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                            >  1:3282650/1‑101 (MQ=255)


                                                             cGAACCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                         <  2:3813901/101‑1 (MQ=255)


                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                         <  1:3368731/101‑1 (MQ=255)


                                                             cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                         >  1:1407711/1‑101 (MQ=255)


                                                               aaaCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                       >  1:1253006/1‑101 (MQ=255)


                                                                  cTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                    <  2:2850602/101‑1 (MQ=255)


                                                                   tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                   <  2:2790534/101‑1 (MQ=255)


                                                                    gcgcCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                  <  2:2582519/101‑1 (MQ=255)


                                                                    gcaccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                  >  1:2563201/4‑101 (MQ=255)


                                                                      gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGGGTTCATGGCCTACCTCGGGCAGTGGACTGGTc                                >  2:2446176/1‑101 (MQ=255)


                                                                       ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                               <  1:4119824/101‑1 (MQ=255)


                                                                         gccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCCTGGCCTACGTCGGGCAGCGGACTGGGCGCt                             >  1:2297049/1‑101 (MQ=255)


                                                                            tATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGTGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                          >  2:2046569/1‑101 (MQ=255)


                                                                             aTCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                         >  1:1900248/1‑101 (MQ=255)


                                                                             aTCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                         <  2:3704310/101‑1 (MQ=255)


                                                                               cGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                       <  1:2970611/101‑1 (MQ=255)


                                                                               cGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                       >  2:282878/1‑101 (MQ=255)


                                                                                gCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                      >  2:260335/1‑101 (MQ=255)


                                                                                    ccGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                  <  1:497600/101‑1 (MQ=255)


                                                                                     cGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                 <  2:234122/101‑1 (MQ=255)


                                                                                      gg...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                >  1:2433647/1‑101 (MQ=255)


                                                                                       g...TC.G..AGCGCCGCGCA.GTCGCGCCCCCCGCGGTGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc               <  2:4070673/101‑1 (MQ=255)


                                                                                            c.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg             >  1:569194/1‑100 (MQ=255)


                                                                                            c.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCgg             >  2:3374230/1‑100 (MQ=255)


                                                                                                 aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGTgg           >  2:1962108/1‑101 (MQ=255)


                                                                                                   cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt         >  1:2696060/1‑101 (MQ=255)


                                                                                                   cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGAt         >  2:1590873/1‑101 (MQ=255)


                                                                                                   cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGCGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt         >  2:3001654/1‑101 (MQ=255)


                                                                                                    gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTTCGTCGGGCAGGTGACTGGTCGATCCCCCCGCGGGGGCGTGGAtt        <  2:2563038/101‑2 (MQ=37)


                                                                                                    gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc        >  2:3890713/1‑101 (MQ=255)


                                                                                                       gggcA.GGCGCTCCCCGCGCGGGGGCGTGGACCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  1:2800031/99‑1 (MQ=255)


                                                                                                       gcgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:1900124/101‑1 (MQ=255)


                                                                                                       cggcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     >  2:1320678/3‑101 (MQ=255)


                                                                                                         gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  2:1766417/101‑1 (MQ=255)


                                                                                                         gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  2:80239/101‑1 (MQ=255)


                                                                                                         gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc   >  2:386098/1‑101 (MQ=255)


                                                                                                          cA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt  >  2:659004/1‑101 (MQ=255)


TGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT  >  NC_014034/1395797‑1395997

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG