Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,931 G→A 13.5% intergenic (+743/‑188) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9310G→A13.5% 79.6 ‑0.2 ‑0.1239intergenic (+743/‑188)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/17):  ref base (102/102):  total (119/120)
Fisher's exact test strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.71e-01

Only 100 of 257 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395832‑1396031
                                                                                                    |                                                                                                    
ggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                                                     <  1:2137740/99‑1 (MQ=255)
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                                                     >  1:247030/1‑101 (MQ=255)
gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                                                     <  1:2207258/101‑1 (MQ=255)
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                                                                    >  1:4003535/1‑101 (MQ=255)
 tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                                                                    >  2:2711793/1‑101 (MQ=9)
 tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                                                                    <  2:1115217/101‑1 (MQ=9)
  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                                                   >  1:3476528/1‑101 (MQ=255)
  cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                                                                   <  1:3603408/101‑1 (MQ=9)
   gccccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                                                                  <  1:1691305/98‑1 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                                                                  >  2:3363969/1‑101 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                                                                  <  2:710377/101‑1 (MQ=255)
   gCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                                                                  >  1:2245784/1‑101 (MQ=9)
    cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                                                                 >  1:640151/1‑101 (MQ=255)
    cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                                                                 >  1:1513585/1‑101 (MQ=9)
     tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                                                                >  1:1886760/4‑100 (MQ=9)
     tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                                                                >  2:1848907/4‑100 (MQ=9)
      ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                               <  1:509517/101‑1 (MQ=255)
      ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                               <  2:727917/101‑1 (MQ=255)
        ccccGCGGGGGCGTGGATCGCAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                             <  2:1311323/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                                                                            >  1:1269005/1‑96 (MQ=9)
         cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                            >  1:1953648/1‑101 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                            <  2:1348696/101‑1 (MQ=255)
            gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggc                                                                                         >  2:3028904/1‑93 (MQ=2)
            gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                         <  1:2605373/101‑9 (MQ=2)
            gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                         <  1:2761300/101‑9 (MQ=2)
            gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                         >  2:2595820/1‑93 (MQ=2)
             cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                                                        >  2:805355/1‑101 (MQ=255)
              gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                                                                       >  1:2387063/1‑91 (MQ=2)
              gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                                                                       <  1:2941047/101‑11 (MQ=2)
              gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       <  1:2664475/101‑1 (MQ=255)
                gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     <  1:1245223/101‑1 (MQ=255)
                gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     >  2:123923/1‑101 (MQ=255)
                  gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                   <  2:4408423/101‑1 (MQ=255)
                    gTGTATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                 <  2:2305763/101‑1 (MQ=255)
                    gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                 >  1:803500/1‑101 (MQ=255)
                    gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                 >  1:1945047/1‑101 (MQ=255)
                     tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                                                                >  2:2889806/1‑101 (MQ=255)
                     tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                                                                >  2:4306281/1‑101 (MQ=255)
                      ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                               >  1:686999/1‑101 (MQ=255)
                       gATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc                                                                              >  1:3282650/1‑101 (MQ=255)
                          cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                           >  1:1407711/1‑101 (MQ=255)
                          cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                           <  1:308008/101‑1 (MQ=255)
                          cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                                                           >  1:363687/1‑101 (MQ=255)
                          cGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACGATGGGTTCATGGCCTACGTCGGGCCGt                                                                           >  1:1102730/1‑101 (MQ=38)
                            aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                         <  2:2849441/101‑1 (MQ=255)
                             aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                        <  2:3205327/101‑1 (MQ=255)
                               cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                      >  2:4061802/1‑101 (MQ=255)
                                tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                     <  2:2790534/101‑1 (MQ=255)
                                tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                     <  1:1146497/101‑1 (MQ=255)
                                 gcaccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                    >  1:2563201/4‑101 (MQ=255)
                                   gccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                  >  1:3359068/1‑101 (MQ=255)
                                        cTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                             <  2:4014313/101‑1 (MQ=255)
                                         tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGTGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            >  2:2046569/1‑101 (MQ=255)
                                         tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            >  1:234149/1‑101 (MQ=255)
                                         tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            <  2:1523071/101‑1 (MQ=255)
                                          aTCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                           <  2:3704310/101‑1 (MQ=255)
                                             gCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGCTCGCTCCCCCcg                                                        <  1:3187453/101‑1 (MQ=255)
                                             ccATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                        <  2:4437812/100‑1 (MQ=255)
                                               aTCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGTCAGTGGACTGGTCGCTCCCCCcgcg                                                      <  2:2410725/101‑1 (MQ=255)
                                                   ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                  >  1:2433647/1‑101 (MQ=255)
                                                     tCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTACATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg                                                >  2:2366195/1‑101 (MQ=255)
                                                        aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGTgg                                             >  2:1962108/1‑101 (MQ=255)
                                                          cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGCGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:3001654/1‑101 (MQ=255)
                                                           gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTTCGTCGGGCAGGTGACTGGTCGATCCCCCCGCGGGGGCGTGGAtt                                          <  2:2563038/101‑2 (MQ=37)
                                                           gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:2405030/1‑101 (MQ=255)
                                                               cgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:2004518/101‑1 (MQ=255)
                                                                gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  2:80239/101‑1 (MQ=255)
                                                                gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  2:3808864/1‑101 (MQ=255)
                                                                gcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                     >  2:386098/1‑101 (MQ=255)
                                                                 cA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                    >  2:659004/1‑101 (MQ=255)
                                                                  a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                   >  1:1010208/1‑101 (MQ=255)
                                                                  a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                   <  1:985951/101‑1 (MQ=255)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGc                                 >  1:1857202/1‑101 (MQ=255)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                <  2:363648/101‑1 (MQ=255)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGccc                               >  2:377845/1‑101 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                              <  2:1660811/101‑1 (MQ=255)
                                                                            ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGAt                          >  2:1939354/1‑101 (MQ=255)
                                                                              ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                        <  2:2171290/101‑1 (MQ=255)
                                                                              ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                        >  2:3415726/1‑101 (MQ=255)
                                                                               cgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcg                       <  2:871724/101‑1 (MQ=255)
                                                                                gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcccc                      >  1:1441713/1‑99 (MQ=255)
                                                                                    gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                  >  1:472376/1‑101 (MQ=255)
                                                                                     ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                 >  2:2530040/1‑101 (MQ=255)
                                                                                     ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                 <  1:3724217/101‑1 (MQ=255)
                                                                                         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             >  2:1131072/1‑101 (MQ=255)
                                                                                         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             >  2:610534/1‑101 (MQ=255)
                                                                                         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             <  1:2836411/101‑1 (MQ=255)
                                                                                         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             <  1:3678366/101‑1 (MQ=255)
                                                                                         tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             <  2:4089290/101‑1 (MQ=255)
                                                                                          ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa            >  1:1193431/1‑101 (MQ=255)
                                                                                          ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa            <  1:4071072/101‑1 (MQ=255)
                                                                                           gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc           <  1:3989989/101‑1 (MQ=255)
                                                                                            tctgAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          <  2:3590414/98‑1 (MQ=255)
                                                                                            aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          <  1:3001875/101‑1 (MQ=255)
                                                                                            aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          >  2:2627684/1‑101 (MQ=255)
                                                                                             tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc         >  1:1793186/1‑101 (MQ=255)
                                                                                              cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc        >  1:2798980/1‑101 (MQ=255)
                                                                                              cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc        >  1:4255778/1‑101 (MQ=255)
                                                                                               gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCCGGCCt       >  2:2673400/1‑101 (MQ=255)
                                                                                                aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg      <  1:4131187/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395832‑1396031

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG