Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,022 C→G 18.2% intergenic (+834/‑97) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0220C→G18.2% 115.2 ‑0.3 ‑0.3247intergenic (+834/‑97)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/24):  ref base (101/101):  total (122/125)
Fisher's exact test strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.47e-01

Only 100 of 267 total aligned reads displayed.

GATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395922‑1396121
                                                                                                    |                                                                                                      
 tctgAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                                                                                                       <  2:3590414/98‑1 (MQ=255)
  tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                                                                                                      >  1:1793186/1‑101 (MQ=255)
    gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCCGGCCt                                                                                                    >  2:2673400/1‑101 (MQ=255)
       aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                                                <  2:1855746/101‑1 (MQ=255)
        cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cg                                                                                               <  1:356041/101‑1 (MQ=255)
         gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                                              >  1:2657680/1‑101 (MQ=255)
         gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                                              <  2:68587/101‑1 (MQ=255)
          aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc..g                                                                                           >  2:2057486/1‑101 (MQ=255)
            gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                         <  2:738869/101‑1 (MQ=255)
            gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                         >  1:1707607/1‑101 (MQ=255)
            gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                         <  2:4255509/101‑1 (MQ=255)
               ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCt                                                                                      >  1:2794556/1‑101 (MQ=255)
                tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTc                                                                                     >  2:3091856/1‑101 (MQ=255)
                tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTc                                                                                     >  1:2254699/1‑101 (MQ=255)
                  aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTccc                                                                                   <  1:4292336/101‑1 (MQ=255)
                  aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTccc                                                                                   >  1:3974156/1‑101 (MQ=255)
                   tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccc                                                                                  <  1:4432791/101‑1 (MQ=255)
                     gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccccc                                                                                >  2:4175934/1‑101 (MQ=255)
                       cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                              >  2:1642874/1‑101 (MQ=255)
                        tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                             >  2:1204448/1‑101 (MQ=255)
                             cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                        >  1:2414294/1‑101 (MQ=255)
                             cGGGCACTGGCCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                        <  1:1411453/101‑1 (MQ=255)
                              gggcgctGGACGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                       <  2:458466/95‑1 (MQ=255)
                              gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                       >  2:2936971/1‑101 (MQ=255)
                              gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                       <  1:4171922/101‑1 (MQ=255)
                               ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                      >  2:3725621/1‑101 (MQ=255)
                                 cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:2872129/1‑101 (MQ=255)
                                 cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGGgg                                                                    >  1:3099716/1‑101 (MQ=255)
                                 cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:3797051/101‑1 (MQ=255)
                                  aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:4114503/101‑1 (MQ=255)
                                   tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  2:1962375/94‑1 (MQ=2)
                                    gggccTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:805437/97‑1 (MQ=255)
                                    ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:2782328/95‑1 (MQ=2)
                                    ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  1:1206894/7‑101 (MQ=2)
                                    ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  2:2262128/7‑101 (MQ=9)
                                     tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:2228383/96‑1 (MQ=2)
                                     ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:3364206/101‑1 (MQ=255)
                                      ggcTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  2:1931421/99‑1 (MQ=255)
                                      gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  2:1045072/97‑1 (MQ=9)
                                        gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:2621254/3‑101 (MQ=9)
                                         tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  2:3647511/1‑101 (MQ=255)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           >  1:370351/1‑101 (MQ=255)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  2:895378/101‑1 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                           <  2:2601028/101‑2 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                          >  1:332353/1‑99 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                          <  1:4045844/101‑3 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                        >  1:1106700/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                       <  1:1450777/101‑1 (MQ=255)
                                               ccccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                      <  2:4401617/99‑1 (MQ=255)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                       <  1:2511230/101‑6 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                      >  1:4007634/1‑101 (MQ=255)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                      >  1:2976215/1‑95 (MQ=2)
                                                cccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                     <  1:3791005/100‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                    >  2:4002412/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                    <  1:2943269/101‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                    <  1:570019/101‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                    >  1:479338/1‑101 (MQ=255)
                                                  cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                    >  1:698258/1‑93 (MQ=2)
                                                  cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                    <  2:3869892/101‑9 (MQ=2)
                                                  cccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                                                   <  1:2690371/101‑1 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                   <  1:3584246/101‑10 (MQ=2)
                                                   ccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                                                  <  2:530923/101‑1 (MQ=255)
                                                     ccGGGGGGGCGTGGAACGAACCTCGCCCTCGATCCGCAGTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                                                <  2:1076025/101‑1 (MQ=37)
                                                       gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                              <  1:4363254/101‑1 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                              <  1:1261945/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACAGGCGCATCTGATGt                                             >  2:2109647/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                         >  2:640299/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                         >  2:3378788/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                        >  1:1655262/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                        >  1:4230488/1‑101 (MQ=255)
                                                              cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                       >  2:3223389/1‑101 (MQ=255)
                                                               gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                      >  1:1557179/1‑101 (MQ=255)
                                                               gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                      >  1:1325017/1‑101 (MQ=255)
                                                                tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                     <  2:1407604/101‑1 (MQ=255)
                                                                   aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                                  >  2:2323381/1‑101 (MQ=255)
                                                                    tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                 <  2:2404873/101‑1 (MQ=255)
                                                                       aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                              >  1:1050816/1‑101 (MQ=255)
                                                                       aaaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                              >  1:4191386/1‑101 (MQ=255)
                                                                       aaaCTCGCCCTCGATCCGCCCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                              <  2:1417291/101‑1 (MQ=255)
                                                                        aaCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                             <  2:1692564/101‑1 (MQ=255)
                                                                            cGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                         >  2:1140814/1‑101 (MQ=255)
                                                                              cccTCGATCCGCGCTTGCCTCGCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                       <  1:1628783/101‑1 (MQ=255)
                                                                              cccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                       <  2:2419983/101‑1 (MQ=255)
                                                                              cccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                       <  1:2333836/101‑1 (MQ=255)
                                                                               ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                      <  2:1360472/101‑1 (MQ=255)
                                                                               ccTCGATCCGCGCTTGCCCCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                      <  1:2057623/101‑1 (MQ=255)
                                                                                 tCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                    >  1:91717/1‑101 (MQ=255)
                                                                                   gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                  <  1:2446333/101‑1 (MQ=255)
                                                                                      ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc               <  1:2627855/101‑1 (MQ=255)
                                                                                      ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc               <  1:66496/101‑1 (MQ=255)
                                                                                      ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc               >  2:1665769/1‑101 (MQ=255)
                                                                                        gcgcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc             <  2:3171617/101‑1 (MQ=255)
                                                                                         cgcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg            <  1:1677953/101‑1 (MQ=255)
                                                                                          gcTTGCCTCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGCGCGTGGATCGAAACAGGCGCATCTGATGTGTATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc           <  2:2179257/101‑1 (MQ=255)
                                                                                          gcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc           <  2:2091212/101‑1 (MQ=255)
                                                                                             tGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCggg        >  1:191382/1‑101 (MQ=255)
                                                                                                cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc     >  1:3484033/1‑101 (MQ=255)
                                                                                                  cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGCGCATCTGATGTATATGCCCGGGGAGAGGTCTGTCACTCCCCCCGCGGGGGCGt   >  2:1501019/1‑101 (MQ=37)
                                                                                                  cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCg  >  1:2176049/1‑100 (MQ=255)
                                                                                                  cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt   <  2:1252913/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
GATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395922‑1396121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG