Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,133 T→G 3.4% F5L (TTT→TCG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1330T→G 3.4% 9.6 ‑1.3 ‑0.0204F5L (TTT→TTGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (103/94):  total (105/99)
Fisher's exact test strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.78e-01

Only 100 of 221 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGG  >  NC_014034/1396033‑1396232
                                                                                                    |                                                                                                      
   cTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCAGCGGGGGCGTGGATCGAAACTTTTCg                                                                                                     >  1:2691866/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGTTTTCGt                                                                                                    >  2:3552349/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGt                                                                                                    >  1:1169197/1‑101 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGt                                                                                                    <  1:3879188/101‑1 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGt                                                                                                    <  2:842510/101‑1 (MQ=255)
    cccccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGt                                                                                                    <  2:1557074/100‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTc                                                                                                   >  1:3922479/1‑101 (MQ=255)
       ccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.At                                                                                                >  1:1077595/1‑101 (MQ=255)
       ccccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.At                                                                                                >  1:1422032/1‑101 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATc                                                                                               <  2:1010123/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                             <  1:610602/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                             <  1:3647764/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                             <  2:2479308/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTg                                                                                             <  2:925021/101‑1 (MQ=255)
            cgGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.c                                                                                          <  1:2353508/101‑1 (MQ=255)
              ggggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAg                                                                                        <  2:2252540/101‑1 (MQ=255)
               gggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAgg                                                                                       <  2:3055752/101‑1 (MQ=255)
                ggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGc                                                                                      <  1:1944447/101‑1 (MQ=255)
                 gCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCt                                                                                     <  2:2635775/101‑1 (MQ=255)
                  cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                    <  1:377884/101‑1 (MQ=255)
                  cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                    >  2:873481/1‑101 (MQ=255)
                  cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                    >  1:3279647/1‑101 (MQ=255)
                   gTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcg                                                                                   <  2:4003279/101‑1 (MQ=255)
                    tGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgc                                                                                  >  1:534464/1‑101 (MQ=255)
                     ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACTTTTCGTC.ATCTGG.CAGGCTcgcc                                                                                 >  2:1276125/1‑100 (MQ=255)
                      gATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCgcgc                                                                                >  1:1863847/1‑101 (MQ=255)
                       aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                               >  1:3205611/1‑101 (MQ=255)
                        tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                              <  2:3994801/101‑1 (MQ=255)
                         cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                             >  1:4269882/1‑101 (MQ=255)
                          gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCcccgcc                                                                            >  1:3050384/1‑101 (MQ=255)
                            aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                          <  2:1324920/101‑1 (MQ=255)
                            aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                          <  1:1204541/101‑1 (MQ=255)
                             aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACg                                                                         <  2:1953519/101‑1 (MQ=255)
                              cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.c                                                                       >  1:621348/1‑101 (MQ=255)
                                ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcccacccg                                                                      >  1:3099263/1‑95 (MQ=255)
                                 gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGc                                                                    <  1:3417775/101‑1 (MQ=255)
                                     aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                >  2:2475520/1‑101 (MQ=255)
                                     aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                >  2:4092765/1‑101 (MQ=255)
                                      tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CCGCGTCGc                                                               >  1:1022697/1‑101 (MQ=255)
                                      tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGc                                                               >  2:3630665/1‑101 (MQ=255)
                                       cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCt                                                              <  2:1055562/101‑1 (MQ=255)
                                          aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTccc                                                           >  2:4107885/1‑101 (MQ=255)
                                          aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTccc                                                           <  2:3901952/101‑1 (MQ=255)
                                          aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGCCGCTccc                                                           >  1:3897358/1‑101 (MQ=255)
                                             tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCTCCACG.CTGCGTCGCTcccccc                                                        <  1:2542267/101‑1 (MQ=255)
                                              atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcg                                                       <  1:3478740/101‑1 (MQ=255)
                                               tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                      >  1:562775/1‑101 (MQ=255)
                                               tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                      >  2:4191456/1‑101 (MQ=255)
                                                    ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggggg                                                 >  1:2857460/1‑101 (MQ=255)
                                                     nggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTcccccccnnnngnn                                                >  1:726035/2‑93 (MQ=255)
                                                     cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggggg                                                >  2:3887366/1‑100 (MQ=255)
                                                     cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGc                                                <  2:3710766/101‑1 (MQ=255)
                                                      ggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCg                                               <  2:3679738/101‑1 (MQ=255)
                                                        tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTg                                             >  1:1586227/11‑101 (MQ=9)
                                                         gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                            <  2:4401344/92‑1 (MQ=2)
                                                         gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTgg                                            <  2:3584602/101‑1 (MQ=255)
                                                         gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTgg                                            >  2:1643282/1‑101 (MQ=255)
                                                         gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCCGGGGCGGgg                                            >  2:1499785/1‑101 (MQ=255)
                                                           gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:2046706/101‑1 (MQ=255)
                                                           gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACC.CTGCGTCGCTCCCCCCCCGGGGGCGGgggt                                          >  1:1716904/1‑99 (MQ=255)
                                                            aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:2475677/101‑1 (MQ=255)
                                                            aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  2:3091917/101‑1 (MQ=255)
                                                              ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                       >  1:2187927/1‑101 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcc                                   >  2:470635/1‑101 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTTTTCGCC.AACTCG.CAGGCCCGCCCCCCCACGCCTGC.CCTCTCCCTCCGGGGGGCGGTGGATGGGAAcc                                   >  2:2366674/1‑101 (MQ=9)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                  <  1:92079/101‑1 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                  <  1:4032507/101‑1 (MQ=255)
                                                                   tCGCTCCCCCAGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGCGGAACGAAAccc                                  >  2:954530/1‑101 (MQ=255)
                                                                    cGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACCCg                                 <  1:2419018/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                               <  1:190951/101‑1 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                            <  1:1004755/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                           >  2:4298928/1‑101 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                          >  1:329609/1‑101 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                          <  1:3552597/101‑1 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                        <  2:3358831/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                      >  1:28233/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                      >  2:174180/1‑101 (MQ=255)
                                                                                 ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                    >  1:1380032/1‑101 (MQ=255)
                                                                                 ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                    >  1:96515/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                   <  2:3483793/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                  <  2:534412/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                  <  2:2010350/101‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                  <  2:2622726/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAGACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                 <  1:1276218/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc               >  1:849340/1‑101 (MQ=255)
                                                                                       tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt              <  1:659072/101‑1 (MQ=255)
                                                                                       tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt              <  2:1169106/101‑1 (MQ=255)
                                                                                       tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATACCt              >  2:2973448/1‑101 (MQ=255)
                                                                                        ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc             >  2:2137757/1‑101 (MQ=255)
                                                                                         gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc            >  2:1592997/1‑101 (MQ=255)
                                                                                          aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg           >  1:4071857/1‑101 (MQ=255)
                                                                                          aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg           >  2:1709740/1‑101 (MQ=255)
                                                                                            cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg         <  1:470110/101‑1 (MQ=255)
                                                                                             gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgggg        >  1:744698/1‑100 (MQ=255)
                                                                                             gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgggg        >  2:649611/1‑100 (MQ=255)
                                                                                               aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt      <  2:2871921/101‑1 (MQ=255)
                                                                                                aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc     <  2:2798774/101‑1 (MQ=255)
                                                                                                aCTTTTCCTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc     <  2:2179115/101‑1 (MQ=255)
                                                                                                  ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg   >  1:3509715/1‑101 (MQ=255)
                                                                                                  ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg   <  1:1537340/101‑1 (MQ=255)
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TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGG  >  NC_014034/1396033‑1396232

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG