Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,148 G→C 3.5% R10S (AGG→AGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1480G→C 3.5% 7.7 ‑1.4 ‑0.2199R10S (AGG→AGCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (106/86):  total (108/91)
Fisher's exact test strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.69e-01

Only 100 of 216 total aligned reads displayed.

GGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCC  >  NC_014034/1396048‑1396246
                                                                                                      |                                                                                                   
gggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAgg                                                                                                     <  1:139562/101‑1 (MQ=255)
 ggCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGc                                                                                                    <  1:1944447/101‑1 (MQ=255)
   cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                                  >  2:3720915/1‑101 (MQ=255)
   cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                                  >  2:873481/1‑101 (MQ=255)
   cGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTc                                                                                                  <  2:2943950/101‑1 (MQ=255)
    gTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcg                                                                                                 <  2:4003279/101‑1 (MQ=255)
     tGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgc                                                                                                >  1:534464/1‑101 (MQ=255)
      ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                               >  1:2532987/1‑101 (MQ=255)
        aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                             <  1:701668/101‑1 (MQ=255)
        aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                             >  1:3205611/1‑101 (MQ=255)
         tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                                            <  2:370312/101‑1 (MQ=255)
         tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccc                                                                                            >  1:256790/1‑100 (MQ=255)
           gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCcccgcc                                                                                          >  1:3050384/1‑101 (MQ=255)
             aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                        <  2:1421925/101‑1 (MQ=255)
             aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                        <  2:2296904/101‑1 (MQ=255)
              aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAcc                                                                                       >  1:3092141/1‑100 (MQ=255)
              aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACg                                                                                       <  1:4066658/101‑1 (MQ=255)
                 ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTg                                                                                   <  2:1193340/101‑1 (MQ=255)
                  gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGc                                                                                  <  1:3417775/101‑1 (MQ=255)
                    gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCgg                                                                                >  1:3500820/1‑100 (MQ=255)
                    gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGt                                                                                >  2:1760031/1‑101 (MQ=255)
                     cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCCCACG.CTGCGTc                                                                               >  1:938107/1‑101 (MQ=255)
                      aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                              >  2:2475520/1‑101 (MQ=255)
                       tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CCGCGTCGc                                                                             >  1:1022697/1‑101 (MQ=255)
                        cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCt                                                                            <  2:1055562/101‑1 (MQ=255)
                           aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTccc                                                                         >  1:3549689/1‑101 (MQ=255)
                           aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGCCGCTccc                                                                         >  1:3897358/1‑101 (MQ=255)
                            tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACG.CTGCGTCGCTcccc                                                                        >  2:470058/1‑101 (MQ=255)
                               atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcg                                                                     <  2:127865/101‑1 (MQ=255)
                               atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcg                                                                     <  2:1712331/101‑1 (MQ=255)
                                tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                                    >  1:562775/1‑101 (MQ=255)
                                tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CCGGGTCGCTCCCCCcgc                                                                    >  2:284957/1‑101 (MQ=255)
                                 atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCC.CCGCGGCCCCCCCCCcccg                                                                   >  1:892226/1‑101 (MQ=25)
                                 atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgcg                                                                   <  1:3704234/101‑1 (MQ=255)
                                   gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                                 <  1:1156947/101‑1 (MQ=255)
                                   gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                                 >  1:647074/1‑101 (MQ=255)
                                    cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                <  1:1215641/101‑1 (MQ=255)
                                    cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                >  2:3591668/1‑101 (MQ=255)
                                    cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACC.CTGCGCCGCTCCCCCC.Cggggg                                                               >  1:1367707/1‑101 (MQ=255)
                                     ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGGCGCTCCCCCCGCggggg                                                               >  1:3965988/1‑101 (MQ=255)
                                          gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:3899311/92‑1 (MQ=2)
                                          gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:616963/92‑1 (MQ=2)
                                          gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCCGGGGCGGgg                                                          >  2:1499785/1‑101 (MQ=255)
                                          gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCACACG.CTGCGTCGCTCCCCCCGCGGGGGGGTgg                                                          >  1:3866181/1‑101 (MQ=255)
                                           agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCTCCGCCACG.CTCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  2:2180607/1‑101 (MQ=255)
                                           agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTggg                                                         <  2:1590442/101‑2 (MQ=255)
                                            gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:2046706/101‑1 (MQ=255)
                                            gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACC.CTGCGTCGCTCCCCCCCCGGGGGCGGgggt                                                        >  1:1716904/1‑99 (MQ=255)
                                             tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCCTGGATc                                                       >  1:727353/7‑101 (MQ=2)
                                             aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:3091917/101‑1 (MQ=255)
                                             aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  2:2697953/1‑101 (MQ=255)
                                               ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCCCGGGGGCGTGGATCGa                                                     >  1:845441/1‑101 (MQ=255)
                                                 cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAttcaaa                                                   >  1:3280479/1‑96 (MQ=255)
                                                 cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  1:4268349/1‑101 (MQ=255)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGGGGGGATCGCAAcc                                                 >  1:4202618/1‑101 (MQ=255)
                                                   gTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTTTTCGCC.AACTCG.CAGGCCCGCCCCCCCACGCCTGC.CCTCTCCCTCCGGGGGGCGGTGGATGGGAAcc                                                 >  2:2366674/1‑101 (MQ=9)
                                                    tCGCTCCCCCAGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGCGGAACGAAAccc                                                >  2:954530/1‑101 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCg                                               >  2:3905634/1‑101 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGACCGAAACTTTTTGTC.ATCTGG.CCTGCGCTCGCCGCCGCG.CTGCGCCACTCCCCCCGCGGGGGCGTGGagccaagaccg                                               >  2:1979134/1‑91 (MQ=21)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                             <  1:190951/101‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGa                                           >  1:2192144/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                                          <  1:1004755/101‑1 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                         >  2:4298928/1‑101 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                        <  2:4096821/101‑1 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                      <  2:4191125/101‑1 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                      <  2:3358831/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                    >  1:28233/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACCCGGGGAAAAACGGc                                   >  2:4216315/1‑101 (MQ=255)
                                                                   gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                 <  2:3483793/101‑1 (MQ=255)
                                                                    ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                <  2:534412/101‑1 (MQ=255)
                                                                    ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                <  2:2622726/101‑1 (MQ=255)
                                                                     gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                               <  1:1633590/101‑1 (MQ=255)
                                                                      cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                              >  2:3567995/1‑101 (MQ=255)
                                                                       gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                             >  1:2469021/1‑101 (MQ=255)
                                                                        tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                            <  1:2366345/101‑1 (MQ=255)
                                                                         ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                           >  2:2137757/1‑101 (MQ=255)
                                                                         ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                           <  2:1128865/101‑1 (MQ=255)
                                                                          gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCCCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                          <  2:824080/101‑1 (MQ=255)
                                                                            tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                        >  2:1634177/1‑101 (MQ=255)
                                                                             cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg                       <  1:470110/101‑1 (MQ=255)
                                                                              gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgggg                      >  2:649611/1‑100 (MQ=255)
                                                                              gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgt                      <  2:3643236/101‑1 (MQ=255)
                                                                                aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt                    <  2:2871921/101‑1 (MQ=255)
                                                                                 aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                   <  2:2798774/101‑1 (MQ=255)
                                                                                   ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                 <  1:985873/101‑1 (MQ=255)
                                                                                   ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                 <  1:1537340/101‑1 (MQ=255)
                                                                                   ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCCGGGGTCTg                 >  2:3201355/1‑101 (MQ=255)
                                                                                      tCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGACTCCGGGTGTCTGGCt              >  2:2990862/1‑101 (MQ=255)
                                                                                       cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg             >  2:156754/1‑101 (MQ=255)
                                                                                       cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTg             >  2:3071407/1‑101 (MQ=255)
                                                                                        gTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTgg            <  2:3965732/101‑2 (MQ=255)
                                                                                        gTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGt            >  2:1668812/1‑101 (MQ=255)
                                                                                          c.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg          <  1:2937192/101‑1 (MQ=255)
                                                                                            aTCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc         >  2:2496218/1‑101 (MQ=255)
                                                                                                gg.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc     <  1:1979263/101‑1 (MQ=255)
                                                                                                   cAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc   <  1:3157705/101‑1 (MQ=255)
                                                                                                    aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc  >  1:3435522/1‑101 (MQ=255)
                                                                                                    aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc  >  2:1943024/1‑101 (MQ=255)
                                                                                                    aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc  <  1:756955/101‑1 (MQ=255)
                                                                                                    aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTcccccc  >  2:922547/1‑101 (MQ=255)
                                                                                                      |                                                                                                   
GGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCC  >  NC_014034/1396048‑1396246

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG