Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,472 A→T 5.4% G118G (GGA→GCT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4720A→T 5.4% 24.5 ‑2.1 ‑0.9203G118G (GGA→GGTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (88/103):  total (96/107)
Fisher's exact test strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.12e-01

Only 100 of 215 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAA  >  NC_014034/1396372‑1396572
                                                                                                    |                                                                                                      
    ccccccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.Tg                                                                                                   <  1:2944784/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.Tg                                                                                                   >  2:1867928/1‑101 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGaaa                                                                                                <  1:346199/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAAt                                                                                               >  2:3457740/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAAtt                                                                                              <  2:4191064/101‑1 (MQ=255)
          gcgGGGGGGTGGATCGAAACCGCTCCCTGCGGCTGATGGCGACGGGTACCGCGGGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTc                                                                                             <  2:453165/101‑1 (MQ=37)
            gggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCcg                                                                                           <  1:4363096/101‑1 (MQ=255)
            gggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCcg                                                                                           >  1:206977/1‑101 (MQ=255)
             ggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCcgc                                                                                          <  1:449237/101‑1 (MQ=255)
             ggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCcgc                                                                                          <  2:2104686/101‑1 (MQ=255)
               ggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCgg                                                                                        <  1:2797402/101‑1 (MQ=255)
               ggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCgg                                                                                        >  1:1201009/1‑101 (MQ=255)
                  gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                     <  2:2582080/101‑1 (MQ=255)
                      aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgc                                                                                 <  2:2852706/101‑1 (MQ=255)
                       tCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgcg                                                                                <  1:757709/101‑1 (MQ=255)
                         gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                              >  2:1559833/1‑101 (MQ=255)
                         gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                              <  2:1958735/101‑1 (MQ=255)
                         gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGCCGCTACCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                              >  1:1249967/1‑101 (MQ=255)
                          aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                             >  1:215409/1‑101 (MQ=255)
                           aaCCGCCCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCGCCCGCGGGGGCGGCGATCGAAACGGACC.TGGAATTCCGAGGGAAACgcggcgc                                                                            >  2:710119/1‑100 (MQ=255)
                                cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                       >  2:1539486/1‑101 (MQ=255)
                                  cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                     >  2:1684114/1‑101 (MQ=255)
                                  cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                     <  1:2262518/101‑1 (MQ=255)
                                   ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                   >  1:3239671/1‑101 (MQ=255)
                                   ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                   <  1:2428015/101‑1 (MQ=255)
                                    cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.Gt                                                                  >  1:3273411/1‑101 (MQ=255)
                                      gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                <  1:4290709/101‑1 (MQ=255)
                                       cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAG.GTCGc                                                               >  2:3117329/1‑101 (MQ=255)
                                       cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGc                                                               >  1:2525168/1‑101 (MQ=255)
                                          gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                            >  2:2025512/1‑101 (MQ=255)
                                           tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                           >  1:2709613/1‑101 (MQ=255)
                                           tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTccc                                                           >  1:1806124/1‑101 (MQ=255)
                                            gATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccc                                                          <  1:557946/101‑1 (MQ=255)
                                              tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                        <  2:1005819/101‑1 (MQ=255)
                                              tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                        <  1:1654634/101‑1 (MQ=255)
                                              tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                        <  2:1335886/101‑1 (MQ=255)
                                               ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                       >  1:4187471/1‑101 (MQ=255)
                                                   aCGGGCACCACGGCGGTCGCTCCCCCCCGGGGGGCGGGGA.CGGAACGGGCCTTGAAATTCCCGGGGAAAGCGGGCGGCGGGC.C.GGCGCTCCCCCCCCgggg                                                  >  2:3566931/1‑101 (MQ=255)
                                                    cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                  >  1:699924/1‑101 (MQ=255)
                                                     gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                 >  2:3067359/1‑101 (MQ=255)
                                                     gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                 <  1:494071/101‑1 (MQ=255)
                                                     gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                 <  1:3591916/101‑1 (MQ=255)
                                                      ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGGGACC.TGAAATTCCGCGGGGAATGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                >  1:3805944/1‑101 (MQ=255)
                                                       gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                               >  2:2671276/1‑101 (MQ=255)
                                                         accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                             <  1:4299197/101‑1 (MQ=255)
                                                         accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                             >  2:2569929/1‑101 (MQ=255)
                                                         accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                             <  1:956191/101‑1 (MQ=255)
                                                          cttccgatctCGCACCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                            <  2:3689453/92‑1 (MQ=38)
                                                          ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                            <  1:3246819/101‑1 (MQ=255)
                                                            tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  2:2926361/7‑101 (MQ=2)
                                                            acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:38229/101‑1 (MQ=255)
                                                            acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  2:84265/101‑1 (MQ=255)
                                                            acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          >  2:3284226/1‑101 (MQ=255)
                                                             cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  2:27172/1‑101 (MQ=255)
                                                              ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        >  1:1932351/1‑101 (MQ=255)
                                                              ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        >  2:399550/1‑101 (MQ=255)
                                                               gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                       >  2:3633853/1‑101 (MQ=255)
                                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                     <  2:405988/101‑1 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                    <  1:2671448/101‑1 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                  >  1:132077/1‑99 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                  >  2:826650/1‑99 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGagatcggaag                                  >  1:3689706/1‑92 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                 <  2:2285844/101‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                >  1:861533/1‑97 (MQ=0)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                >  1:1826570/1‑97 (MQ=0)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                >  2:4428057/1‑97 (MQ=0)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                >  2:4376495/1‑101 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                >  2:1186934/1‑101 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                               <  2:4322048/101‑6 (MQ=0)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                               <  2:553227/101‑6 (MQ=0)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                               >  1:3582712/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                               <  1:2050827/101‑1 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGCTCC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAAcaccggc                              >  1:3751175/1‑95 (MQ=0)
                                                                        ccccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                              >  2:661692/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                             >  2:3542081/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                             <  2:889485/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCg                            <  2:3786017/101‑1 (MQ=255)
                                                                           cccGCGGGGGCGTGGATCGAAACAGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTCGt                           <  1:4148103/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                          <  2:2748458/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                          <  2:3708735/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                          <  2:1237441/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                       <  2:804244/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                       <  2:1249874/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                      >  2:1584674/1‑101 (MQ=255)
                                                                                gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGAGat                      >  1:1959311/1‑100 (MQ=255)
                                                                                 ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                     <  1:4026033/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                     >  1:741481/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                <  2:3626573/101‑1 (MQ=255)
                                                                                       tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg               >  2:1723480/1‑101 (MQ=255)
                                                                                        ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc              >  1:664905/1‑101 (MQ=255)
                                                                                        ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc              >  2:343466/1‑101 (MQ=255)
                                                                                         gATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc             <  2:2422072/101‑1 (MQ=255)
                                                                                          aTCGAAACGGACC.TGAAAGTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg            <  2:483537/101‑1 (MQ=255)
                                                                                             gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc         <  2:3759377/101‑1 (MQ=255)
                                                                                             gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc         >  2:3030819/1‑101 (MQ=255)
                                                                                             gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc         <  1:1047790/101‑1 (MQ=255)
                                                                                              aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg        >  1:3392202/1‑101 (MQ=255)
                                                                                               aaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc       <  2:2877478/101‑1 (MQ=255)
                                                                                               aaCGGACC.TGAAATTCCGCGGGAAAAGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc       >  2:567031/1‑101 (MQ=255)
                                                                                                   gACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa   >  1:3042563/1‑101 (MQ=255)
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CGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAA  >  NC_014034/1396372‑1396572

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG