Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,486 G→C 5.8% R123P (CGG→CCA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4860G→C 5.8% 18.4 ‑1.5 ‑0.0188R123P (CGG→CCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (88/89):  total (96/92)
Fisher's exact test strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.96e-01

Only 100 of 209 total aligned reads displayed.

GGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGC  >  NC_014034/1396387‑1396586
                                                                                                    |                                                                                                     
ggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCgg                                                                                                      >  1:1201009/1‑101 (MQ=255)
ggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCgg                                                                                                      <  1:2797402/101‑1 (MQ=255)
 gCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCggg                                                                                                     <  1:156024/101‑1 (MQ=255)
 gCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCggg                                                                                                     <  1:2012233/101‑1 (MQ=255)
   gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                                   <  2:2582080/101‑1 (MQ=255)
   gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                                   >  1:2553633/1‑101 (MQ=255)
   gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGATCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                                   <  1:3698147/101‑1 (MQ=255)
       aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgc                                                                                               <  2:2852706/101‑1 (MQ=255)
       aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgc                                                                                               >  1:2512934/1‑101 (MQ=255)
        tCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgcg                                                                                              <  1:757709/101‑1 (MQ=255)
          gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                            <  2:1958735/101‑1 (MQ=255)
          gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                            >  1:3607450/1‑101 (MQ=255)
           aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                           >  2:1545095/1‑101 (MQ=255)
           aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                           >  1:215409/1‑101 (MQ=255)
            aaCCGCCCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCGCCCGCGGGGGCGGCGATCGAAACGGACC.TGGAATTCCGAGGGAAACgcggcgc                                                                                          >  2:710119/1‑100 (MQ=255)
              ccGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcggcg                                                                                        >  2:3302961/1‑101 (MQ=255)
               cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                       <  2:3629792/101‑1 (MQ=255)
               cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGCC.TGAAATTCCGCCGGAGACgcggcggcca                                                                                       >  2:1573400/1‑99 (MQ=37)
                gcgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAg                                                                                      <  2:1693431/101‑1 (MQ=255)
                  gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                                    <  2:3635654/101‑1 (MQ=255)
                   cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                   <  1:295585/101‑1 (MQ=255)
                    ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                 >  1:1237534/1‑101 (MQ=255)
                    ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                 <  1:2428015/101‑1 (MQ=255)
                    ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                 >  1:3239671/1‑101 (MQ=255)
                     cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.Gt                                                                                >  1:3273411/1‑101 (MQ=255)
                       gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                              >  1:1301844/1‑101 (MQ=255)
                       gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                              >  2:3940587/1‑101 (MQ=255)
                        cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAG.GTCGc                                                                             >  2:3117329/1‑101 (MQ=255)
                          ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                           >  1:2064400/1‑101 (MQ=255)
                           gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                          >  2:2025512/1‑101 (MQ=255)
                            tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTccc                                                                         >  1:1806124/1‑101 (MQ=255)
                                ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccca                                                                     >  2:1654528/1‑100 (MQ=255)
                                    aCGGGCACCACGGCGGTCGCTCCCCCCCGGGGGGCGGGGA.CGGAACGGGCCTTGAAATTCCCGGGGAAAGCGGGCGGCGGGC.C.GGCGCTCCCCCCCCgggg                                                                >  2:3566931/1‑101 (MQ=255)
                                      gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                               >  2:3067359/1‑101 (MQ=255)
                                      gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                               <  1:3591916/101‑1 (MQ=255)
                                        gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                             >  2:3715976/1‑101 (MQ=255)
                                        gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                             >  2:2671276/1‑101 (MQ=255)
                                          accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:3794514/101‑1 (MQ=255)
                                          accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:4299197/101‑1 (MQ=255)
                                          accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:956191/101‑1 (MQ=255)
                                          accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                           >  2:2569929/1‑101 (MQ=255)
                                          accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:2677001/1‑101 (MQ=255)
                                           cttccgatctCGCACCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:3689453/92‑1 (MQ=38)
                                           ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:452768/101‑1 (MQ=255)
                                           ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:3246819/101‑1 (MQ=255)
                                             tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  2:2926361/7‑101 (MQ=2)
                                             acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:84265/101‑1 (MQ=255)
                                             acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  2:3284226/1‑101 (MQ=255)
                                             acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:38229/101‑1 (MQ=255)
                                              cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  2:27172/1‑101 (MQ=255)
                                               ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  2:399550/1‑101 (MQ=255)
                                               ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:1932351/1‑101 (MQ=255)
                                               ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGCGGATCg                                                      >  2:1374548/1‑101 (MQ=255)
                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  1:570823/101‑1 (MQ=255)
                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:3760315/101‑1 (MQ=255)
                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:1217914/101‑1 (MQ=255)
                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:405988/101‑1 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                 >  2:610814/1‑101 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                               <  2:2285844/101‑1 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                              >  1:861533/1‑97 (MQ=0)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                              >  1:1826570/1‑97 (MQ=0)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                              >  2:1186934/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                              >  2:4376495/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                                             <  2:553227/101‑6 (MQ=0)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                                             <  2:4322048/101‑6 (MQ=0)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                             >  1:3582712/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                           <  2:889485/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                        <  2:2748458/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                     <  2:804244/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                     <  1:3457980/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                     >  2:3491650/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCCC.GTCGTTCCCCCCGCGGGGGCGCGGATCGAAACTGCTTTCGTGGGAc                                    >  2:4429082/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                    >  1:701329/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                   <  1:4026033/101‑1 (MQ=255)
                                                                  ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                   >  1:741481/1‑101 (MQ=255)
                                                                        tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                             >  1:683274/1‑101 (MQ=255)
                                                                         ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                            <  1:4406534/101‑1 (MQ=255)
                                                                          gATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                           <  2:2422072/101‑1 (MQ=255)
                                                                          gATCGAAACCGACC.TGAAATTCCGCGGGAACCGCGGCGGCGAGCCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                           <  1:4164600/101‑1 (MQ=255)
                                                                           aTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                          >  1:553782/1‑101 (MQ=255)
                                                                           aTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                          >  2:4203516/1‑101 (MQ=255)
                                                                           aTCGAAACGGACC.TGAAAGTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                          <  2:483537/101‑1 (MQ=255)
                                                                             cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                        <  2:2114405/101‑1 (MQ=255)
                                                                              gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                       <  1:1047790/101‑1 (MQ=255)
                                                                              gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                       >  2:3030819/1‑101 (MQ=255)
                                                                              gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                       >  1:4274337/1‑101 (MQ=255)
                                                                               aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                      <  2:1301747/101‑1 (MQ=255)
                                                                                aaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                     <  2:2877478/101‑1 (MQ=255)
                                                                                aaCGGACC.TGAAATTCCGCGGGAAAAGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                     >  2:567031/1‑101 (MQ=255)
                                                                                  cGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCgcgc                   <  1:4198157/101‑1 (MQ=255)
                                                                                    gACC.CGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                 <  2:1240579/101‑1 (MQ=255)
                                                                                     aCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCaa                <  1:3174943/101‑1 (MQ=255)
                                                                                           aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc           >  2:3553818/1‑101 (MQ=255)
                                                                                           aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc           <  2:699844/101‑1 (MQ=255)
                                                                                            aagtCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt          <  1:1684225/98‑1 (MQ=255)
                                                                                            aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt          >  2:830669/1‑101 (MQ=255)
                                                                                             aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc         >  2:1771130/1‑101 (MQ=255)
                                                                                             aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc         >  2:1089386/1‑101 (MQ=255)
                                                                                                 cgcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc     >  1:27100/1‑101 (MQ=255)
                                                                                                  gcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc    >  2:1619467/1‑101 (MQ=255)
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GGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGC  >  NC_014034/1396387‑1396586

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG