Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,489 A→C 5.7% E124A (GAA→ACA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4890A→C 5.7% 22.1 ‑1.5 ‑0.5193E124A (GAA→GCA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (94/87):  total (103/90)
Fisher's exact test strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.26e-01

Only 100 of 212 total aligned reads displayed.

CGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGG  >  NC_014034/1396389‑1396589
                                                                                                     |                                                                                                     
 gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                                      <  1:3249876/101‑1 (MQ=255)
 gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGATCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGaa                                                                                                      <  1:3698147/101‑1 (MQ=255)
       cGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcgg                                                                                                >  2:2130882/1‑101 (MQ=255)
        gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                               <  2:1958735/101‑1 (MQ=255)
        gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                               <  2:3110985/101‑1 (MQ=255)
        gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                               >  2:1559833/1‑101 (MQ=255)
        gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                               >  1:3607450/1‑101 (MQ=255)
         aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                              >  1:215409/1‑101 (MQ=255)
               cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                        >  2:1539486/1‑101 (MQ=255)
                gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcgggcagtc                                                                                       <  1:98669/101‑7 (MQ=255)
                 cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                      <  2:3255082/101‑1 (MQ=255)
                 cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                      >  2:1684114/1‑101 (MQ=255)
                 cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                      <  1:2262518/101‑1 (MQ=255)
                  ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                    <  1:2428015/101‑1 (MQ=255)
                   cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.Gt                                                                                   <  2:2930599/101‑1 (MQ=255)
                     gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                                 >  2:3940587/1‑101 (MQ=255)
                     gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                                 >  1:1301844/1‑101 (MQ=255)
                      cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGc                                                                                >  1:2525168/1‑101 (MQ=255)
                       gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGcc                                                                               >  2:2593509/1‑100 (MQ=255)
                        ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                              >  1:2064400/1‑101 (MQ=255)
                         gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                             >  2:2025512/1‑101 (MQ=255)
                         gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                             >  1:3559207/1‑101 (MQ=255)
                          tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                            >  1:2709613/1‑101 (MQ=255)
                          tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                            >  2:4268755/1‑101 (MQ=255)
                          tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                            >  1:835709/1‑101 (MQ=255)
                          tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTccc                                                                            >  1:1806124/1‑101 (MQ=255)
                               gCGACGGGCACCCCGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcgc                                                                       <  2:3066934/101‑1 (MQ=255)
                                cGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcgcg                                                                      >  2:1652878/1‑101 (MQ=255)
                                 ggcGGGCACCCCGGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgg                                                                     <  1:927802/99‑1 (MQ=255)
                                  aCGGGCACCACGGCGGTCGCTCCCCCCCGGGGGGCGGGGA.CGGAACGGGCCTTGAAATTCCCGGGGAAAGCGGGCGGCGGGC.C.GGCGCTCCCCCCCCgggg                                                                   >  2:3566931/1‑101 (MQ=255)
                                     ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGGGACC.TGAAATTCCGCGGGGAATGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                                 >  1:3805944/1‑101 (MQ=255)
                                      gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                >  2:3715976/1‑101 (MQ=255)
                                        accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                              <  1:4299197/101‑1 (MQ=255)
                                         cttccgatctCGCACCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:3689453/92‑1 (MQ=38)
                                         ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:452768/101‑1 (MQ=255)
                                          cacGGCGGTCGCCCCCCCCGCGGGGGCGGGGATCGGAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:3209629/101‑1 (MQ=255)
                                           acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:84265/101‑1 (MQ=255)
                                             ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:1932351/1‑101 (MQ=255)
                                              gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:3633853/1‑101 (MQ=255)
                                               cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  1:570823/101‑1 (MQ=255)
                                               cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:3760315/101‑1 (MQ=255)
                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:2772131/1‑101 (MQ=255)
                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:4406262/1‑101 (MQ=255)
                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:1217914/101‑1 (MQ=255)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:2671448/101‑1 (MQ=255)
                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                                   >  2:4045584/1‑99 (MQ=2)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                   >  2:2695441/1‑101 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                  <  1:27185/101‑1 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                 >  1:1826570/1‑97 (MQ=0)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                                 >  2:1186934/1‑101 (MQ=255)
                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                                                <  2:553227/101‑6 (MQ=0)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACGCTCC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAAcaccggc                                               >  1:3751175/1‑95 (MQ=0)
                                                       ccccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                               >  2:661692/1‑101 (MQ=255)
                                                        cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                              <  2:889485/101‑1 (MQ=255)
                                                              cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                        <  2:804244/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCCC.GTCGTTCCCCCCGCGGGGGCGCGGATCGAAACTGCTTTCGTGGGAc                                       >  2:4429082/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGTAc                                       <  1:3303193/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       >  2:1584674/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       >  2:3293067/1‑101 (MQ=255)
                                                               gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       >  1:701329/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                      <  2:292594/101‑1 (MQ=255)
                                                                ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                      >  1:741481/1‑101 (MQ=255)
                                                                 gggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                                     <  2:2525005/101‑1 (MQ=255)
                                                                  ggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTgg                                    <  2:2859433/101‑1 (MQ=255)
                                                                     gTGGATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                 <  2:3626573/101‑1 (MQ=255)
                                                                     gTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                 >  2:763393/1‑101 (MQ=255)
                                                                      tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                >  1:683274/1‑101 (MQ=255)
                                                                      tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                >  2:1723480/1‑101 (MQ=255)
                                                                      tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                <  2:707756/101‑1 (MQ=255)
                                                                      tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                <  2:4337350/101‑1 (MQ=255)
                                                                       ggATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCCGCGAGCAC.GTCGCCCCCCCCGCGGGGGCGTGGGTCGAACCTGCTTTCGTGGGACTGGCCAgc                               <  2:2729188/101‑1 (MQ=38)
                                                                       ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                               <  1:4406534/101‑1 (MQ=255)
                                                                       ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                               >  2:343466/1‑101 (MQ=255)
                                                                       ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                               >  2:4095314/1‑101 (MQ=255)
                                                                        gATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                              <  2:2422072/101‑1 (MQ=255)
                                                                        gATCGAAACCGACC.TGAAATTCCGCGGGAACCGCGGCGGCGAGCCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                              <  1:4164600/101‑1 (MQ=255)
                                                                         aTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                             >  1:553782/1‑101 (MQ=255)
                                                                         aTCGAAACGGACC.TGAAAGTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                             <  2:483537/101‑1 (MQ=255)
                                                                           cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                           <  1:324361/101‑1 (MQ=255)
                                                                           cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                           >  1:3589218/1‑101 (MQ=255)
                                                                            gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                          <  1:1047790/101‑1 (MQ=255)
                                                                            gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                          >  1:4274337/1‑101 (MQ=255)
                                                                             aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                         >  1:3392202/1‑101 (MQ=255)
                                                                             aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                         <  2:1301747/101‑1 (MQ=255)
                                                                                cGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCgcgc                      <  1:4198157/101‑1 (MQ=255)
                                                                                 ggACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGcc                     >  2:2577149/1‑101 (MQ=255)
                                                                                  gACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                    >  1:3042563/1‑101 (MQ=255)
                                                                                  gACC.CGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                    <  2:1240579/101‑1 (MQ=255)
                                                                                         aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc              >  1:122189/1‑101 (MQ=255)
                                                                                         aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc              >  2:3553818/1‑101 (MQ=255)
                                                                                          aagtCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt             <  1:1684225/98‑1 (MQ=255)
                                                                                          aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt             >  2:830669/1‑101 (MQ=255)
                                                                                           aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc            >  2:1771130/1‑101 (MQ=255)
                                                                                           aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc            >  2:1089386/1‑101 (MQ=255)
                                                                                                 cgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg      <  1:3940841/101‑1 (MQ=255)
                                                                                                  gggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc     >  2:573674/1‑101 (MQ=255)
                                                                                                   ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg    <  1:1559938/101‑1 (MQ=255)
                                                                                                   ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg    >  1:1243366/1‑101 (MQ=255)
                                                                                                   ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg    >  2:3667426/1‑101 (MQ=255)
                                                                                                    gAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg   <  1:4095574/101‑1 (MQ=255)
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CGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGG  >  NC_014034/1396389‑1396589

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG