Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,493 G→T 6.0% T125T (ACG→AAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4930G→T 6.0% 20.2 ‑1.5 ‑0.5184T125T (ACG→ACTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (91/82):  total (99/85)
Fisher's exact test strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.91e-01

Only 100 of 214 total aligned reads displayed.

ATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396394‑1396593
                                                                                                    |                                                                                                     
aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgc                                                                                                      <  2:2852706/101‑1 (MQ=255)
 tCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAAcgcg                                                                                                     <  1:757709/101‑1 (MQ=255)
  cGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcgg                                                                                                    >  2:2130882/1‑101 (MQ=255)
   gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                                   >  2:1559833/1‑101 (MQ=255)
   gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                                   <  2:1958735/101‑1 (MQ=255)
   gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                                   >  1:3607450/1‑101 (MQ=255)
    aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                                  <  1:1782205/101‑1 (MQ=255)
     aaCCGCCCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCGCCCGCGGGGGCGGCGATCGAAACGGACC.TGGAATTCCGAGGGAAACgcggcgc                                                                                                 >  2:710119/1‑100 (MQ=255)
          cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                            <  1:4307788/101‑1 (MQ=255)
            cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                          >  2:1684114/1‑101 (MQ=255)
            cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                          <  1:2262518/101‑1 (MQ=255)
            cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                          <  1:295585/101‑1 (MQ=255)
             ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                        <  1:2428015/101‑1 (MQ=255)
             ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                        >  1:3239671/1‑101 (MQ=255)
                 cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAG.GTCGc                                                                                    >  2:3117329/1‑101 (MQ=255)
                 cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGc                                                                                    >  1:2525168/1‑101 (MQ=255)
                   ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                                  >  1:2070678/1‑101 (MQ=255)
                    gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                 >  2:2025512/1‑101 (MQ=255)
                     tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                >  2:4268755/1‑101 (MQ=255)
                     tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                >  1:835709/1‑101 (MQ=255)
                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                             <  2:1005819/101‑1 (MQ=255)
                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                             <  2:1335886/101‑1 (MQ=255)
                        tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                             <  1:1654634/101‑1 (MQ=255)
                         ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                                            >  2:1173729/1‑101 (MQ=255)
                           cGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcgcg                                                                          >  2:1652878/1‑101 (MQ=255)
                            ggcGGGCACCCCGGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgg                                                                         <  1:927802/99‑1 (MQ=255)
                             aCGGGCACCACGGCGGTCGCTCCCCCCCGGGGGGCGGGGA.CGGAACGGGCCTTGAAATTCCCGGGGAAAGCGGGCGGCGGGC.C.GGCGCTCCCCCCCCgggg                                                                       >  2:3566931/1‑101 (MQ=255)
                               gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                      <  1:3591916/101‑1 (MQ=255)
                               gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                      <  1:494071/101‑1 (MQ=255)
                               gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                      >  2:3067359/1‑101 (MQ=255)
                                 gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                    >  2:2671276/1‑101 (MQ=255)
                                  caccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGt                                                                   <  2:3686933/101‑1 (MQ=255)
                                   accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                  <  1:3794514/101‑1 (MQ=255)
                                   accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                  >  1:2677001/1‑101 (MQ=255)
                                    ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:3246819/101‑1 (MQ=255)
                                      tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  2:2926361/7‑101 (MQ=2)
                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:84265/101‑1 (MQ=255)
                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  1:38229/101‑1 (MQ=255)
                                      acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  2:3284226/1‑101 (MQ=255)
                                       cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  2:27172/1‑101 (MQ=255)
                                        ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  2:399550/1‑101 (MQ=255)
                                        ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:1932351/1‑101 (MQ=255)
                                         gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  2:3633853/1‑101 (MQ=255)
                                          cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:570823/101‑1 (MQ=255)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  2:405988/101‑1 (MQ=255)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:4406262/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                                       >  1:132077/1‑99 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                      <  1:27185/101‑1 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                                                    <  2:553227/101‑6 (MQ=0)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                    >  1:3582712/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                    <  1:2050827/101‑1 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                  <  2:889485/101‑1 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                  <  1:1809551/101‑1 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCg                                                 <  2:3786017/101‑1 (MQ=255)
                                                      ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                               <  2:1237441/101‑1 (MQ=255)
                                                      ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                               <  2:3708735/101‑1 (MQ=255)
                                                      ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                               <  2:2748458/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                            <  2:804244/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                            <  2:1249874/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                            >  2:3491650/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCCC.GTCGTTCCCCCCGCGGGGGCGCGGATCGAAACTGCTTTCGTGGGAc                                           >  2:4429082/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGTAc                                           <  1:3303193/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                           >  1:4291686/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                           >  1:701329/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                           >  2:1584674/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                          >  1:741481/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                          <  1:4026033/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                                         <  2:2525005/101‑1 (MQ=255)
                                                                gTGGATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                     <  2:3626573/101‑1 (MQ=255)
                                                                gTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                     >  2:763393/1‑101 (MQ=255)
                                                                 tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                    <  2:4337350/101‑1 (MQ=255)
                                                                 tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                    >  1:683274/1‑101 (MQ=255)
                                                                 tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                    >  2:1723480/1‑101 (MQ=255)
                                                                   gATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                  <  2:2422072/101‑1 (MQ=255)
                                                                   gATCGAAACCGACC.TGAAATTCCGCGGGAACCGCGGCGGCGAGCCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                  <  1:4164600/101‑1 (MQ=255)
                                                                    aTCGAAACGGACC.TGAAAGTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                                 <  2:483537/101‑1 (MQ=255)
                                                                       gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                              <  2:3759377/101‑1 (MQ=255)
                                                                       gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                              <  1:1047790/101‑1 (MQ=255)
                                                                       gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                              >  1:4274337/1‑101 (MQ=255)
                                                                       gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                              >  2:3030819/1‑101 (MQ=255)
                                                                        aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                             >  1:3392202/1‑101 (MQ=255)
                                                                         aaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                            <  2:2877478/101‑1 (MQ=255)
                                                                           cGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCgcgc                          <  1:4198157/101‑1 (MQ=255)
                                                                            ggACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGcc                         >  2:2577149/1‑101 (MQ=255)
                                                                              aCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCaa                       <  1:3174943/101‑1 (MQ=255)
                                                                                    aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                  >  2:3553818/1‑101 (MQ=255)
                                                                                    aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                  <  2:699844/101‑1 (MQ=255)
                                                                                    aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                  >  1:122189/1‑101 (MQ=255)
                                                                                     aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                 >  2:830669/1‑101 (MQ=255)
                                                                                      aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                >  2:1771130/1‑101 (MQ=255)
                                                                                         ccGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc             >  1:451277/1‑101 (MQ=255)
                                                                                           gcgGGAAACGCGGCGGCGAGCAC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc           <  2:1200918/101‑1 (MQ=255)
                                                                                             gggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc         >  2:573674/1‑101 (MQ=255)
                                                                                              ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg        >  1:1243366/1‑101 (MQ=255)
                                                                                                aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg      >  1:3715078/1‑101 (MQ=255)
                                                                                                 aaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg     >  2:3370717/1‑101 (MQ=255)
                                                                                                 aaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCCCGCCCAGCCGCTCCCCCCGCgggg     >  2:3954264/1‑101 (MQ=255)
                                                                                                   cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc   <  2:1512149/101‑1 (MQ=255)
                                                                                                   cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc   <  2:3596422/101‑1 (MQ=255)
                                                                                                   cgcgGCGGCGAGCAC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGGGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc   <  1:1254435/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
ATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396394‑1396593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG