Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,495 G→T 6.0% R126L (CGG→ATG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4950G→T 6.0% 18.1 ‑1.5 ‑0.9184R126L (CGG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (91/82):  total (99/85)
Fisher's exact test strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.14e-01

Only 100 of 216 total aligned reads displayed.

TCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396395‑1396595
                                                                                                    |                                                                                                     
tCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAcgcg                                                                                                       <  1:757709/101‑1 (MQ=255)
  gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggc                                                                                                     <  2:3110985/101‑1 (MQ=255)
   aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcg                                                                                                    >  1:3596670/1‑101 (MQ=255)
   aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcg                                                                                                    >  1:215409/1‑101 (MQ=255)
   aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcg                                                                                                    >  2:1545095/1‑101 (MQ=255)
       cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                                <  2:3629792/101‑1 (MQ=255)
       cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGCCTGAAATTCCGCCGGAGACgcggcggcca                                                                                                >  2:1573400/1‑99 (MQ=37)
        gcgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAg                                                                                               <  2:1693431/101‑1 (MQ=255)
         cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                              <  1:4307788/101‑1 (MQ=255)
          gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                                             <  2:3635654/101‑1 (MQ=255)
           cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                            <  2:3255082/101‑1 (MQ=255)
               gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                                       >  2:3940587/1‑101 (MQ=255)
                 gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGcc                                                                                     >  2:2593509/1‑100 (MQ=255)
                  ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                                    >  1:2064400/1‑101 (MQ=255)
                   gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                   >  1:3559207/1‑101 (MQ=255)
                   gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                   >  2:2025512/1‑101 (MQ=255)
                    tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                  >  2:4268755/1‑101 (MQ=255)
                    tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                  >  1:835709/1‑101 (MQ=255)
                     gATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccc                                                                                 <  1:557946/101‑1 (MQ=255)
                       tGGCGACGGGCACCACGGCGCTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                               <  2:2773348/101‑1 (MQ=255)
                        ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccca                                                                              >  2:1654528/1‑100 (MQ=255)
                        ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                                              >  1:4187471/1‑101 (MQ=255)
                           ggcGGGCACCCCGGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgg                                                                           <  1:927802/99‑1 (MQ=255)
                             cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                                         >  2:1744702/1‑101 (MQ=255)
                              gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                        <  1:494071/101‑1 (MQ=255)
                               ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                                       <  2:3607202/101‑1 (MQ=255)
                                 caccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGt                                                                     <  2:3686933/101‑1 (MQ=255)
                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:3794514/101‑1 (MQ=255)
                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    >  1:2677001/1‑101 (MQ=255)
                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    >  2:2569929/1‑101 (MQ=255)
                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:956191/101‑1 (MQ=255)
                                   ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:3246819/101‑1 (MQ=255)
                                    cacGGCGGTCGCCCCCCCCGCGGGGGCGGGGATCGGAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  <  1:3209629/101‑1 (MQ=255)
                                     tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:2926361/7‑101 (MQ=2)
                                     acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:38229/101‑1 (MQ=255)
                                     acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:84265/101‑1 (MQ=255)
                                      cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:27172/1‑101 (MQ=255)
                                       ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGCGGATCg                                                               >  2:1374548/1‑101 (MQ=255)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  2:1217914/101‑1 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                          >  2:610814/1‑101 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                                         >  2:4045584/1‑99 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                                         >  1:132077/1‑99 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                         <  2:552835/101‑1 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                        <  2:2285844/101‑1 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                       >  2:4428057/1‑97 (MQ=0)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                       <  2:475743/101‑5 (MQ=0)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                                       >  2:1186934/1‑101 (MQ=255)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                      >  1:2877686/1‑101 (MQ=255)
                                                  cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                    <  2:889485/101‑1 (MQ=255)
                                                  cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                    <  1:1809551/101‑1 (MQ=255)
                                                    cccGCGGGGGCGTGGATCGAAACAGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTCGt                                                  <  1:4148103/101‑1 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                                 <  2:2748458/101‑1 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                                 <  2:3708735/101‑1 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTgg                                                <  1:1868052/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                              >  2:3491650/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGTAc                                             <  1:3303193/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                             >  2:3293067/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                             >  1:4291686/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                             >  1:701329/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGAGat                                             >  1:1959311/1‑100 (MQ=255)
                                                          ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                            <  1:4026033/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                            >  1:741481/1‑101 (MQ=255)
                                                                tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                      <  2:4337350/101‑1 (MQ=255)
                                                                 ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                                     >  1:664905/1‑101 (MQ=255)
                                                                 ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                                     >  2:4095314/1‑101 (MQ=255)
                                                                     cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                                 <  2:2114405/101‑1 (MQ=255)
                                                                     cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                                 >  1:3589218/1‑101 (MQ=255)
                                                                      gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                <  2:3759377/101‑1 (MQ=255)
                                                                      gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                >  2:3030819/1‑101 (MQ=255)
                                                                      gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                <  1:1047790/101‑1 (MQ=255)
                                                                       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                               <  2:1301747/101‑1 (MQ=255)
                                                                       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                               >  2:1884035/1‑101 (MQ=255)
                                                                       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                               >  1:3392202/1‑101 (MQ=255)
                                                                        aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                              <  2:2877478/101‑1 (MQ=255)
                                                                        aaCGGACCTGAAATTCCGCGGGAAAAGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                              >  2:567031/1‑101 (MQ=255)
                                                                            gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                          >  1:3042563/1‑101 (MQ=255)
                                                                            gACCCGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                          <  2:1240579/101‑1 (MQ=255)
                                                                                  aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                    >  1:122189/1‑101 (MQ=255)
                                                                                  aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                    >  2:3553818/1‑101 (MQ=255)
                                                                                  aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                    <  2:699844/101‑1 (MQ=255)
                                                                                   aagtCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                   <  1:1684225/98‑1 (MQ=255)
                                                                                   aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                   >  2:830669/1‑101 (MQ=255)
                                                                                    aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc                  >  2:1089386/1‑101 (MQ=255)
                                                                                       ccGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc               >  1:451277/1‑101 (MQ=255)
                                                                                        cgcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc              >  2:3971335/1‑101 (MQ=255)
                                                                                        cgcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc              >  1:27100/1‑101 (MQ=255)
                                                                                         gcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc             >  2:1619467/1‑101 (MQ=255)
                                                                                          cgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg            >  1:4208922/1‑101 (MQ=255)
                                                                                            ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg          <  1:1559938/101‑1 (MQ=255)
                                                                                            ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg          >  1:1243366/1‑101 (MQ=255)
                                                                                             gAAACGCGGCGGCGAGCCC.GGCGCTCCCCCCGCGGGGGCTTGTATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg         <  2:801466/101‑1 (MQ=38)
                                                                                             gAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg         >  2:2498898/1‑101 (MQ=255)
                                                                                              aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg        >  1:2398972/1‑101 (MQ=255)
                                                                                              aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg        >  1:3715078/1‑101 (MQ=255)
                                                                                               aaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg       >  2:3370717/1‑101 (MQ=255)
                                                                                               aaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg       <  2:741401/101‑1 (MQ=255)
                                                                                                 cgcgGCGGCGAGCAC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGGGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc     <  1:1254435/101‑1 (MQ=255)
                                                                                                  gcggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg    <  2:3582464/101‑1 (MQ=255)
                                                                                                   cggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg   >  2:820662/1‑100 (MQ=255)
                                                                                                    ggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg  <  1:2636050/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396395‑1396595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG