Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,497 C→T 5.9% R127W (CGG→TGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4970C→T 5.9% 19.4 ‑1.5 ‑0.4185R127W (CGG→TGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (91/83):  total (99/86)
Fisher's exact test strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.66e-01

Only 100 of 218 total aligned reads displayed.

GAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396397‑1396596
                                                                                                     |                                                                                                    
gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                                      <  2:1958735/101‑1 (MQ=255)
gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGCCGCTACCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggc                                                                                                      >  1:1249967/1‑101 (MQ=255)
 aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                                     >  1:3596670/1‑101 (MQ=255)
 aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                                     >  1:215409/1‑101 (MQ=255)
 aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcg                                                                                                     >  2:1545095/1‑101 (MQ=255)
  aaCCGCCCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCGCCCGCGGGGGCGGCGATCGAAACGGACC.TGGAATTCCGAGGGAAACgcggcgc                                                                                                    >  2:710119/1‑100 (MQ=255)
    ccGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACgcggcggcg                                                                                                  >  2:3302961/1‑101 (MQ=255)
     cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGCC.TGAAATTCCGCCGGAGACgcggcggcca                                                                                                 >  2:1573400/1‑99 (MQ=37)
      gcgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAg                                                                                                <  2:1693431/101‑1 (MQ=255)
       cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                               <  1:4307788/101‑1 (MQ=255)
        gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                                              <  2:3635654/101‑1 (MQ=255)
          ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                                           >  1:1237534/1‑101 (MQ=255)
           cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.Gt                                                                                          >  1:3273411/1‑101 (MQ=255)
             gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                                        <  1:4290709/101‑1 (MQ=255)
             gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                                        >  1:1301844/1‑101 (MQ=255)
              cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAG.GTCGc                                                                                       >  2:3117329/1‑101 (MQ=255)
              cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGc                                                                                       >  1:2525168/1‑101 (MQ=255)
               gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGcc                                                                                      >  2:2593509/1‑100 (MQ=255)
                 gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                    >  1:3559207/1‑101 (MQ=255)
                 gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                    >  2:2025512/1‑101 (MQ=255)
                  tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                   >  1:835709/1‑101 (MQ=255)
                  tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                   >  1:2709613/1‑101 (MQ=255)
                  tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                   >  2:4268755/1‑101 (MQ=255)
                  tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTccc                                                                                   >  1:1806124/1‑101 (MQ=255)
                      ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccca                                                                               >  2:1654528/1‑100 (MQ=255)
                      ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                                               >  1:4187471/1‑101 (MQ=255)
                         ggcGGGCACCCCGGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgg                                                                            <  1:927802/99‑1 (MQ=255)
                          aCGGGCACCACGGCGGTCGCTCCCCCCCGGGGGGCGGGGA.CGGAACGGGCCTTGAAATTCCCGGGGAAAGCGGGCGGCGGGC.C.GGCGCTCCCCCCCCgggg                                                                          >  2:3566931/1‑101 (MQ=255)
                           cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                                          >  1:699924/1‑101 (MQ=255)
                            gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                         >  2:3067359/1‑101 (MQ=255)
                               caccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGt                                                                      <  2:3686933/101‑1 (MQ=255)
                                accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                                     <  1:956191/101‑1 (MQ=255)
                                  cacGGCGGTCGCCCCCCCCGCGGGGGCGGGGATCGGAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:3209629/101‑1 (MQ=255)
                                   tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  2:2926361/7‑101 (MQ=2)
                                   acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  2:3284226/1‑101 (MQ=255)
                                   acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:84265/101‑1 (MQ=255)
                                   acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:38229/101‑1 (MQ=255)
                                     ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  1:1932351/1‑101 (MQ=255)
                                       cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  1:570823/101‑1 (MQ=255)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  2:2772131/1‑101 (MQ=255)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:1217914/101‑1 (MQ=255)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  2:4406262/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:2671448/101‑1 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                           >  2:610814/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                                          >  2:4045584/1‑99 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGagatcggaag                                                          >  1:3689706/1‑92 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                          <  2:552835/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                          >  2:2695441/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                        >  1:756548/1‑97 (MQ=0)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                        >  1:1826570/1‑97 (MQ=0)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                        <  2:475743/101‑5 (MQ=0)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCt                                                        >  2:1186934/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                       <  1:2050827/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                       >  1:2877686/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACGCTCC.TGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAAcaccggc                                                      >  1:3751175/1‑95 (MQ=0)
                                                cccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                     <  2:889485/101‑1 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCg                                                    <  2:3786017/101‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACAGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTCGt                                                   <  1:4148103/101‑1 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                                  <  2:3708735/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                               >  2:3491650/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                             <  1:4026033/101‑1 (MQ=255)
                                                         gggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                                            <  2:2525005/101‑1 (MQ=255)
                                                          ggCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTgg                                           <  2:2859433/101‑1 (MQ=255)
                                                             gTGGATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                        <  2:3626573/101‑1 (MQ=255)
                                                              tGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                       <  2:4337350/101‑1 (MQ=255)
                                                               ggATCGAAACGGCCC.TGAAATTCCGCGGGAAACGCGGCCGCGAGCAC.GTCGCCCCCCCCGCGGGGGCGTGGGTCGAACCTGCTTTCGTGGGACTGGCCAgc                                      <  2:2729188/101‑1 (MQ=38)
                                                               ggATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                                      >  2:4095314/1‑101 (MQ=255)
                                                                gATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                     <  2:2422072/101‑1 (MQ=255)
                                                                 aTCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccg                                    >  1:553782/1‑101 (MQ=255)
                                                                   cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                                  <  1:324361/101‑1 (MQ=255)
                                                                   cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                                  <  2:2114405/101‑1 (MQ=255)
                                                                   cGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                                  >  1:3589218/1‑101 (MQ=255)
                                                                    gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                 <  2:3759377/101‑1 (MQ=255)
                                                                    gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                 <  1:1047790/101‑1 (MQ=255)
                                                                    gAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc                                 >  2:3030819/1‑101 (MQ=255)
                                                                     aaaCGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg                                <  2:1301747/101‑1 (MQ=255)
                                                                      aaCGGACC.TGAAATTCCGCGGGAAAAGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc                               >  2:567031/1‑101 (MQ=255)
                                                                          gACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                           >  1:3042563/1‑101 (MQ=255)
                                                                          gACC.CGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa                           <  2:1240579/101‑1 (MQ=255)
                                                                                 aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                     <  2:699844/101‑1 (MQ=255)
                                                                                 aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                     >  2:3553818/1‑101 (MQ=255)
                                                                                  aagtCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                    <  1:1684225/98‑1 (MQ=255)
                                                                                  aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                    >  2:830669/1‑101 (MQ=255)
                                                                                   aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                   >  2:1771130/1‑101 (MQ=255)
                                                                                   aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc                   >  2:1089386/1‑101 (MQ=255)
                                                                                      ccGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATGGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                <  1:710199/101‑1 (MQ=255)
                                                                                      ccGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                >  1:451277/1‑101 (MQ=255)
                                                                                        gcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc              >  2:1619467/1‑101 (MQ=255)
                                                                                         cgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg             >  1:4208922/1‑101 (MQ=255)
                                                                                            gAAACGCGGCGGCGAGCCC.GGCGCTCCCCCCGCGGGGGCTTGTATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg          <  2:801466/101‑1 (MQ=38)
                                                                                             aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg         >  1:338772/1‑101 (MQ=255)
                                                                                             aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg         >  1:2398972/1‑101 (MQ=255)
                                                                                              aaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCCCGCCCAGCCGCTCCCCCCGCgggg        >  2:3954264/1‑101 (MQ=255)
                                                                                                cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc      <  2:1512149/101‑1 (MQ=255)
                                                                                                cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc      <  2:3596422/101‑1 (MQ=255)
                                                                                                  cggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg    >  2:820662/1‑100 (MQ=255)
                                                                                                   ggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg   <  1:1539591/101‑1 (MQ=255)
                                                                                                    gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg  >  1:1623917/1‑101 (MQ=255)
                                                                                                    gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg  <  1:1187025/101‑1 (MQ=255)
                                                                                                    gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg  <  1:467907/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACC.TGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396397‑1396596

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG