BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,539	T→A	16.6%	C141S (TGC→AGC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,539	0	T→A	16.6%	123.5 ‑0.5 ‑0.6	252	C141S (TGC→AGC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/23): ref base (106/104): total (125/127)
Fisher's exact test strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.40e-01

Only 100 of 261 total aligned reads displayed.


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGG  >  NC_014034/1396439‑1396639


tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                        >  2:610814/1‑101 (MQ=255)


  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                      <  1:27185/101‑1 (MQ=255)


  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                      >  2:832123/1‑101 (MQ=255)


      cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                                                                  >  2:3542081/1‑101 (MQ=255)


       ccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.g                                                                                                <  2:3786017/101‑1 (MQ=255)


         ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.Tg                                                                                             <  2:2748458/101‑1 (MQ=255)


         ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.Tg                                                                                             <  2:3708735/101‑1 (MQ=255)


         ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.Tg                                                                                             <  2:954830/101‑1 (MQ=255)


         atctgggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.Tg                                                                                             <  2:1959182/97‑1 (MQ=255)


            cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGa                                                                                          <  2:1249874/101‑1 (MQ=255)


             gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCCCGTCGTTCCCCCCGCGGGGGCGCGGATCGAAACTGCTTTC.G.TGGGAc                                                                                         >  2:4429082/1‑101 (MQ=255)


             gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGAc                                                                                         >  1:4291686/1‑101 (MQ=255)


             gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGAc                                                                                         >  1:701329/1‑101 (MQ=255)


               gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTg                                                                                       <  2:2525005/101‑1 (MQ=255)


                ggCGTTGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTgg                                                                                      <  2:3558959/101‑1 (MQ=255)


                    tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                  <  2:4337350/101‑1 (MQ=255)


                     ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgc                                                                                 >  2:4095314/1‑101 (MQ=255)


                      gATCGAAACCGACCTGAAATTCCGCGGGAACCGCGGCGGCGAGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                <  1:4164600/101‑1 (MQ=255)


                       aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                               >  1:553782/1‑101 (MQ=255)


                       aTCGAAACGGACCTGAAAGTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                               <  2:483537/101‑1 (MQ=255)


                         cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccgcc                                                                             >  1:3589218/1‑101 (MQ=255)


                          gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                            >  2:3030819/1‑101 (MQ=255)


                            aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccgc                                                                          <  2:2877478/101‑1 (MQ=255)


                            aaCGGACCTGAAATTCCGCGGGAAAAGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccgc                                                                          >  2:567031/1‑101 (MQ=255)


                                 aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCaa                                                                     <  1:3174943/101‑1 (MQ=255)


                                      aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                <  2:699844/101‑1 (MQ=255)


                                       aagtCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                               <  1:1684225/98‑1 (MQ=255)


                                        aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc                                                              >  2:1089386/1‑101 (MQ=255)


                                           ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                                                           >  1:451277/1‑101 (MQ=255)


                                             gcgGGAAACGCGGCGGCGAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                         <  2:1200918/101‑1 (MQ=255)


                                              cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                        >  1:4208922/1‑101 (MQ=255)


                                               gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc                                                       <  1:110320/101‑1 (MQ=255)


                                                 gAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                     <  1:4095574/101‑1 (MQ=255)


                                                  aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                    >  1:338772/1‑101 (MQ=255)


                                                   aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                   >  2:3370717/1‑101 (MQ=255)


                                                   aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCCCGCCCAGCCGCTCCCCCCGCgggg                                                   >  2:3954264/1‑101 (MQ=255)


                                                     cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                 <  2:1512149/101‑1 (MQ=255)


                                                     cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                 <  2:3596422/101‑1 (MQ=255)


                                                     cgcgGCGGCGAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                 <  1:1254435/101‑1 (MQ=255)


                                                       cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg                                               >  2:820662/1‑100 (MQ=255)


                                                        ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                              <  1:1539591/101‑1 (MQ=255)


                                                        ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                              >  1:936148/1‑101 (MQ=255)


                                                          ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                             <  1:1884950/91‑1 (MQ=2)


                                                           tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                            >  1:1615433/10‑101 (MQ=2)


                                                             cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:12401/1‑101 (MQ=255)


                                                             cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:2064263/101‑1 (MQ=255)


                                                             cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGACCg                                         <  1:2554076/101‑1 (MQ=255)


                                                             cgAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:4376766/101‑1 (MQ=255)


                                                              gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                         <  1:2059839/95‑1 (MQ=2)


                                                              gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                         >  2:1155358/7‑101 (MQ=2)


                                                              gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  1:1590526/1‑101 (MQ=255)


                                                              gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:364332/101‑1 (MQ=255)


                                                               aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:3280771/1‑101 (MQ=255)


                                                                gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:1374645/101‑1 (MQ=255)


                                                                gCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                      >  1:735965/1‑101 (MQ=255)


                                                                cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                       <  2:781863/97‑1 (MQ=2)


                                                                 ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGaaa                                      >  1:3976466/4‑101 (MQ=2)


                                                                  gagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGGCAACCTATGTCCCTCACCCCGCGGGGGCGTGGATCGACAc                                     >  2:1866597/3‑101 (MQ=2)


                                                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  2:3237383/100‑1 (MQ=255)


                                                                  ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  1:3067580/100‑1 (MQ=255)


                                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                   >  2:1991920/1‑101 (MQ=255)


                                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                   >  1:1749200/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                  >  1:2790/1‑98 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                >  2:1598266/1‑101 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                >  1:2868377/1‑101 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                 >  2:734698/1‑97 (MQ=2)


                                                                      cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                <  1:212933/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                               >  2:2259416/1‑101 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                               <  1:832205/101‑1 (MQ=255)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                               <  1:3503333/101‑7 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                             <  2:1680624/101‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                            <  1:661760/101‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATTtcggc                            >  2:1336621/1‑99 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                             >  1:123665/1‑93 (MQ=2)


                                                                          ccccccGAGGGGGCGTGGATCGAAACAGCTTTC.GATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                             <  1:1042252/101‑9 (MQ=2)


                                                                           cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                            >  1:2423335/1‑92 (MQ=2)


                                                                           cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                            >  1:19679/1‑92 (MQ=2)


                                                                             cccGCGGGGGCGTCGATCGAAACTGCTTTC.G.TGGGCCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                         <  2:1417937/101‑1 (MQ=255)


                                                                              ccGGGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                        <  2:4274068/101‑1 (MQ=255)


                                                                               cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                       <  2:2676829/101‑1 (MQ=255)


                                                                               cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                       <  2:1623811/101‑1 (MQ=255)


                                                                                gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                      <  1:3575030/101‑1 (MQ=255)


                                                                                gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                      <  1:2695631/101‑1 (MQ=255)


                                                                                    gggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                  >  2:2497328/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                >  1:3762495/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                >  1:3414102/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                <  2:3628751/101‑1 (MQ=255)


                                                                                       cGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt               >  2:2298419/1‑101 (MQ=255)


                                                                                       cGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACGATTTCGTCGCTTACGACGCCt               >  1:3843048/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa              >  2:272369/1‑101 (MQ=255)


                                                                                         tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt             >  1:294865/1‑101 (MQ=255)


                                                                                         tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt             >  2:3428670/1‑101 (MQ=255)


                                                                                          ggATCGAAACTGCTTTC.G.TGGGACTGGCCAGGCGCCCGCGCCAAGCCGCTCCCCCCGCGGGGGCGGGGGTCCGAACCACTTCGTCGGTTCCGCCGGCTATc            >  2:546779/1‑101 (MQ=255)


                                                                                           gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt           <  1:763473/101‑1 (MQ=255)


                                                                                               gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt       >  1:2195027/1‑101 (MQ=255)


                                                                                                aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt      <  1:3284458/101‑1 (MQ=255)


                                                                                                 aaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc     >  1:3928369/1‑101 (MQ=255)


                                                                                                   cTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg   <  2:2398815/101‑1 (MQ=255)


                                                                                                   cTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg   >  2:173185/1‑101 (MQ=255)


                                                                                                    tGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg  <  2:2627365/101‑1 (MQ=255)


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGG  >  NC_014034/1396439‑1396639

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG