Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,553 T→C 17.2% T145T (ACT→GGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5530T→C17.2% 96.3 ‑0.9 0.0243T145T (ACT→ACCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (107/94):  total (126/117)
Fisher's exact test strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653
                                                                                                      |                                                                                                    
ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACt                                                                                                      <  2:292594/101‑1 (MQ=255)
  ggCGTTGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTgg                                                                                                    <  2:3558959/101‑1 (MQ=255)
  ggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTgg                                                                                                    <  2:2859433/101‑1 (MQ=255)
     gTGGATCGAAACGGCCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCa                                                                                                 <  2:3626573/101‑1 (MQ=255)
     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCa                                                                                                 >  2:763393/1‑101 (MQ=255)
      tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                                >  1:683274/1‑101 (MQ=255)
      tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                                >  2:1723480/1‑101 (MQ=255)
      tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                                <  2:4337350/101‑1 (MQ=255)
      tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                                <  2:707756/101‑1 (MQ=255)
       ggATCGAAACGGCCCTGAAATTCCGCGGGAAACGCGGCCGCGAGCACGTCGCCCCCCCCGCGGGGGCGTGGGTCGAACCTGCTTTC.G.TGGGACTGGCCAgc                                                                                               <  2:2729188/101‑1 (MQ=38)
       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgc                                                                                               >  2:343466/1‑101 (MQ=255)
       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgc                                                                                               >  2:4095314/1‑101 (MQ=255)
        gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                              <  2:2422072/101‑1 (MQ=255)
        gATCGAAACCGACCTGAAATTCCGCGGGAACCGCGGCGGCGAGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                              <  1:4164600/101‑1 (MQ=255)
         aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                                             >  1:553782/1‑101 (MQ=255)
           cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccgcc                                                                                           >  1:3589218/1‑101 (MQ=255)
            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                                          >  2:3030819/1‑101 (MQ=255)
            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                                          >  1:4274337/1‑101 (MQ=255)
             aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccg                                                                                         <  2:1301747/101‑1 (MQ=255)
             aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccg                                                                                         >  2:1884035/1‑101 (MQ=255)
                cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCgcgc                                                                                      <  1:4198157/101‑1 (MQ=255)
                 ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGcc                                                                                     >  2:2577149/1‑101 (MQ=255)
                  gACCCGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCa                                                                                    <  2:1240579/101‑1 (MQ=255)
                   aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCaa                                                                                   <  1:3174943/101‑1 (MQ=255)
                         aagtCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                             <  1:1684225/98‑1 (MQ=255)
                          aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCCCCAAGTCGCTc                                                                            >  2:1089386/1‑101 (MQ=255)
                             ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                                                                         >  1:451277/1‑101 (MQ=255)
                              cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                        >  2:3971335/1‑101 (MQ=255)
                               gcgGGAAACGCGGCGGCGAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                       <  2:1200918/101‑1 (MQ=255)
                                 gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgc                                                                     <  1:110320/101‑1 (MQ=255)
                                   gAAACGCGGCGGCGAGCCCGGCGCTCCCCCCGCGGGGGCTTGTATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                   <  2:801466/101‑1 (MQ=38)
                                   gAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                   >  2:2498898/1‑101 (MQ=255)
                                    aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                  >  1:3715078/1‑101 (MQ=255)
                                    aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                  >  1:338772/1‑101 (MQ=255)
                                      aCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggggg                                                                >  1:82613/1‑101 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               <  2:3596422/101‑1 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               <  1:1254435/101‑1 (MQ=255)
                                            ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                           <  1:1884950/91‑1 (MQ=2)
                                             ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  2:1652462/1‑101 (MQ=255)
                                              gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         >  2:946460/9‑101 (MQ=2)
                                              gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:1521635/101‑1 (MQ=255)
                                               cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:3239442/101‑1 (MQ=255)
                                               cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  2:1504049/1‑101 (MQ=255)
                                               cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:2064263/101‑1 (MQ=255)
                                               cgAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:4376766/101‑1 (MQ=255)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       <  1:2059839/95‑1 (MQ=2)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       >  2:1155358/7‑101 (MQ=2)
                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:1590526/1‑101 (MQ=255)
                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      <  1:364332/101‑1 (MQ=255)
                                                 aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:3280771/1‑101 (MQ=255)
                                                  gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:1374645/101‑1 (MQ=255)
                                                  gCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                    >  1:735965/1‑101 (MQ=255)
                                                   cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:3293299/99‑1 (MQ=255)
                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                  <  1:3067580/100‑1 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                >  1:2379599/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              >  2:2042619/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              >  1:2868377/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              <  2:2553470/101‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                              >  2:1598266/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                               <  2:195485/101‑5 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                               <  2:3831300/101‑5 (MQ=2)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             <  2:1749081/101‑1 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                             <  1:3503333/101‑7 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                            >  1:280896/1‑94 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          <  2:3414283/101‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          <  1:399589/101‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATTtcggc                                          >  1:1591481/1‑99 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           <  2:3746239/101‑9 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           >  1:861629/1‑93 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           >  1:123665/1‑93 (MQ=2)
                                                            ccccccGAGGGGGCGTGGATCGAAACAGCTTTC.GATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                           <  1:1042252/101‑9 (MQ=2)
                                                             cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                          >  1:2423335/1‑92 (MQ=2)
                                                             cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                          <  1:3050161/101‑10 (MQ=2)
                                                               cccGCGGGGGCGTCGATCGAAACTGCTTTC.G.TGGGCCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                       <  2:1417937/101‑1 (MQ=255)
                                                                ccGGGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                      <  2:4274068/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                     <  2:2676829/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                     <  1:3415596/101‑1 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    >  1:974813/1‑101 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  1:2695631/101‑1 (MQ=255)
                                                                   ggggggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                   <  1:2772321/100‑1 (MQ=255)
                                                                        gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                              <  2:553730/101‑1 (MQ=255)
                                                                         cGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                             >  2:2298419/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           >  2:2357160/1‑101 (MQ=255)
                                                                           gggATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           <  1:1744821/100‑1 (MQ=255)
                                                                             gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         <  1:3542329/101‑1 (MQ=255)
                                                                             gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         <  1:61078/101‑1 (MQ=255)
                                                                             gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                         <  1:763473/101‑1 (MQ=255)
                                                                              aTCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                        <  2:522006/101‑1 (MQ=255)
                                                                               tCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                       <  2:451237/101‑1 (MQ=255)
                                                                                cGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                      >  1:3913835/1‑101 (MQ=255)
                                                                                cGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                      <  2:683194/101‑1 (MQ=255)
                                                                                  aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGACACGATTTCGTCGCTTACGACGCCTATCTGAttt                    >  1:3819549/1‑101 (MQ=255)
                                                                                  aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    <  1:2131021/101‑1 (MQ=255)
                                                                                         tttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg             >  1:2616436/1‑101 (MQ=255)
                                                                                          ttC.G.TGGGGCTGGCCAGCCTCCCGCGCCAAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc            <  1:388016/101‑1 (MQ=255)
                                                                                            c.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  1:1967909/1‑101 (MQ=255)
                                                                                                   gACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccccc     <  1:2169799/101‑1 (MQ=255)
                                                                                                    aCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGCCGCCTATCTGATTTCGGGTCGCTCCCCCcg    <  1:3971591/101‑1 (MQ=255)
                                                                                                    aCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg    >  2:2241332/1‑101 (MQ=255)
                                                                                                      tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg  >  2:1627515/1‑101 (MQ=255)
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GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG