Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,556 C→G 16.9% G146G (GGC→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5560C→G16.9% 103.8 ‑1.2 ‑0.0249G146G (GGC→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (112/95):  total (131/118)
Fisher's exact test strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 258 total aligned reads displayed.

GTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396458‑1396656
                                                                                                    |                                                                                                    
 tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAg                                                                                                   >  1:683274/1‑101 (MQ=255)
  ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgc                                                                                                  >  2:4095314/1‑101 (MQ=255)
   gATCGAAACCGACCTGAAATTCCGCGGGAACCGCGGCGGCGAGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                                 <  1:4164600/101‑1 (MQ=255)
    aTCGAAACGGACCTGAAAGTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                                                <  2:483537/101‑1 (MQ=255)
      cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccgcc                                                                                              <  1:324361/101‑1 (MQ=255)
       gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                                             <  2:3759377/101‑1 (MQ=255)
       gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                                             >  1:4274337/1‑101 (MQ=255)
        aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccg                                                                                            >  1:3392202/1‑101 (MQ=255)
         aaCGGACCTGAAATTCCGCGGGAAAAGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccgc                                                                                           >  2:567031/1‑101 (MQ=255)
           cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCgcgc                                                                                         <  1:4198157/101‑1 (MQ=255)
            ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGcc                                                                                        >  2:2577149/1‑101 (MQ=255)
                   aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                                 <  2:699844/101‑1 (MQ=255)
                     aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                               >  2:1771130/1‑101 (MQ=255)
                        ccGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccc                                                                            >  1:451277/1‑101 (MQ=255)
                         cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                           >  1:27100/1‑101 (MQ=255)
                             ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcgcg                                                                       >  2:3667426/1‑101 (MQ=255)
                               aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                     >  1:2398972/1‑101 (MQ=255)
                                aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                    >  2:3370717/1‑101 (MQ=255)
                                aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCCCGCCCAGCCGCTCCCCCCGCgggg                                                                    >  2:3954264/1‑101 (MQ=255)
                                   gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg                                                                 <  2:3582464/101‑1 (MQ=255)
                                    cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg                                                                >  2:820662/1‑100 (MQ=255)
                                     ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                               <  1:1539591/101‑1 (MQ=255)
                                      gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:1591343/101‑1 (MQ=255)
                                      gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:1187025/101‑1 (MQ=255)
                                       ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGCgg                                                              >  2:3603160/11‑101 (MQ=2)
                                        tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                             >  1:1615433/10‑101 (MQ=2)
                                        tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                             >  2:1983051/10‑101 (MQ=2)
                                         gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                            >  2:946460/9‑101 (MQ=2)
                                          ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                           <  1:3518542/94‑1 (MQ=2)
                                          cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  2:12401/1‑101 (MQ=255)
                                          cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  2:1504049/1‑101 (MQ=255)
                                          cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  2:3239442/101‑1 (MQ=255)
                                          cgAGCACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4376766/101‑1 (MQ=255)
                                           gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:3907924/1‑101 (MQ=255)
                                            acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                         >  2:1200012/6‑101 (MQ=2)
                                             gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:3620198/101‑1 (MQ=255)
                                             gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:1374645/101‑1 (MQ=255)
                                             gCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                       >  1:735965/1‑101 (MQ=255)
                                             cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                        <  2:781863/97‑1 (MQ=2)
                                             cggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaa                                                        <  1:2480993/97‑1 (MQ=2)
                                              ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGaaa                                                       >  1:3976466/4‑101 (MQ=2)
                                               ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                     <  2:3054932/100‑1 (MQ=255)
                                               aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                     >  1:2627536/1‑101 (MQ=255)
                                                ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                     <  2:4385113/100‑2 (MQ=2)
                                                cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGNGGCGTGGATCGNAANNa                                                    >  2:1147041/1‑101 (MQ=255)
                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                   >  2:1552600/1‑101 (MQ=255)
                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                  >  2:734698/1‑97 (MQ=2)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                >  2:2259416/1‑101 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                >  1:2815314/1‑101 (MQ=255)
                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAG.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                 <  1:826396/101‑6 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGACACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGAGGATCGaaaatgtgccg                                               >  2:1347072/1‑93 (MQ=2)
                                                      tCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccg                                               >  1:3123024/1‑94 (MQ=2)
                                                       ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                             <  2:3414283/101‑1 (MQ=255)
                                                       ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                              >  1:123665/1‑93 (MQ=2)
                                                       ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                              <  2:2925726/101‑9 (MQ=2)
                                                       ccccccGAGGGGGCGTGGATCGAAACAGCTTTC.GATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                              <  1:1042252/101‑9 (MQ=2)
                                                        cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                             <  2:2645069/101‑10 (MQ=2)
                                                         ccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGc                                           >  1:522058/1‑101 (MQ=255)
                                                            cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                        <  2:1623811/101‑1 (MQ=255)
                                                             gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                       <  1:3575030/101‑1 (MQ=255)
                                                               gggggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                     >  2:1290947/1‑101 (MQ=255)
                                                                ggggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                    <  2:2504892/101‑1 (MQ=255)
                                                                 gggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacg                                   >  2:2497328/1‑101 (MQ=255)
                                                                 gggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgacc                                   >  2:2365714/1‑100 (MQ=255)
                                                                  ggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGc                                  >  1:2157769/1‑101 (MQ=255)
                                                                   gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                 >  1:3762495/1‑101 (MQ=255)
                                                                    cGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                >  1:1699990/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               <  2:4208661/101‑1 (MQ=255)
                                                                     gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               >  2:272369/1‑101 (MQ=255)
                                                                     gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                               >  2:1644636/1‑101 (MQ=255)
                                                                      tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              >  1:294865/1‑101 (MQ=255)
                                                                      gggATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              <  1:1744821/100‑1 (MQ=255)
                                                                        gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            <  2:2070541/101‑1 (MQ=255)
                                                                        gATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            <  1:61078/101‑1 (MQ=255)
                                                                         aTCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           <  1:2778486/101‑1 (MQ=255)
                                                                         aTCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           <  2:522006/101‑1 (MQ=255)
                                                                            gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTCTCTGAtt                        >  1:2069828/1‑101 (MQ=255)
                                                                            gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                        >  1:2195027/1‑101 (MQ=255)
                                                                            gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                        >  1:3826154/1‑101 (MQ=255)
                                                                             aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                       >  2:2043152/1‑101 (MQ=255)
                                                                              aaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTc                      >  1:3928369/1‑101 (MQ=255)
                                                                               aCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                     >  1:1278164/1‑101 (MQ=255)
                                                                                cTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                    >  2:173185/1‑101 (MQ=255)
                                                                                  gCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                  >  1:1900834/1‑101 (MQ=255)
                                                                                  gCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                  >  1:93577/1‑101 (MQ=255)
                                                                                  gCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATATGATTTCGGGt                  >  2:1933661/1‑101 (MQ=255)
                                                                                    tttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                <  1:3491890/101‑1 (MQ=255)
                                                                                     ttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc               >  1:788801/1‑101 (MQ=255)
                                                                                     ttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc               >  2:2351128/1‑101 (MQ=255)
                                                                                      tC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCt              <  1:3667679/101‑1 (MQ=255)
                                                                                       c.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTNNGACGCCTATCTGATTTCGGGTCGCTc             >  1:3361325/1‑101 (MQ=255)
                                                                                       c.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc             >  1:1967909/1‑101 (MQ=255)
                                                                                         g.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc            >  2:3441424/1‑101 (MQ=255)
                                                                                         g.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc            >  2:1706204/1‑101 (MQ=255)
                                                                                                cTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc      >  1:3055255/1‑101 (MQ=255)
                                                                                                  ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTTGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg    <  1:1089474/101‑1 (MQ=255)
                                                                                                  ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg    >  2:4276855/1‑101 (MQ=255)
                                                                                                   gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   <  2:2868169/101‑1 (MQ=255)
                                                                                                   gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   <  1:998071/101‑1 (MQ=255)
                                                                                                    ccAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg  >  2:1002776/1‑101 (MQ=255)
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GTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396458‑1396656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG