Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,561 C→G 18.6% P148R (CCG→CGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5610C→G18.6% 105.1 ‑0.9 ‑0.1226P148R (CCG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (98/86):  total (117/109)
Fisher's exact test strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.63e-01

Only 100 of 255 total aligned reads displayed.

GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396461‑1396661
                                                                                                      |                                                                                                    
gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                                      <  2:2422072/101‑1 (MQ=255)
gATCGAAACCGACCTGAAATTCCGCGGGAACCGCGGCGGCGAGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgcc                                                                                                      <  1:4164600/101‑1 (MQ=255)
 aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                                                     >  2:4203516/1‑101 (MQ=255)
 aTCGAAACGGACCTGAAAGTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccg                                                                                                     <  2:483537/101‑1 (MQ=255)
   cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAgccgcc                                                                                                   >  1:3589218/1‑101 (MQ=255)
    gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgccc                                                                                                  >  1:4274337/1‑101 (MQ=255)
     aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccg                                                                                                 >  2:1884035/1‑101 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccgc                                                                                                <  2:2877478/101‑1 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAAAGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGccgcccgc                                                                                                >  2:567031/1‑101 (MQ=255)
         ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGcc                                                                                             >  2:2577149/1‑101 (MQ=255)
          gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCa                                                                                            >  1:3042563/1‑101 (MQ=255)
                aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                                      <  2:699844/101‑1 (MQ=255)
                 aagtCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                     <  1:1684225/98‑1 (MQ=255)
                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                                    >  2:1771130/1‑101 (MQ=255)
                      cgcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccccc                                                                                >  2:3971335/1‑101 (MQ=255)
                       gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                               >  2:1619467/1‑101 (MQ=255)
                        cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                              <  1:3940841/101‑1 (MQ=255)
                        cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                              >  1:4208922/1‑101 (MQ=255)
                           gAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                           >  2:2498898/1‑101 (MQ=255)
                            aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggg                                                                          >  1:3715078/1‑101 (MQ=255)
                             aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                         >  2:3370717/1‑101 (MQ=255)
                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                       <  2:1512149/101‑1 (MQ=255)
                                gcggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCg                                                                      <  2:3582464/101‑1 (MQ=255)
                                 cggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCgg                                                                     >  2:820662/1‑100 (MQ=255)
                                  ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:1539591/101‑1 (MQ=255)
                                  ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:2636050/101‑1 (MQ=255)
                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:467907/101‑1 (MQ=255)
                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:1187025/101‑1 (MQ=255)
                                    ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGCgg                                                                   >  2:3603160/11‑101 (MQ=2)
                                     tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                  >  1:1615433/10‑101 (MQ=2)
                                      gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                                 >  2:946460/9‑101 (MQ=2)
                                       cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:2064263/101‑1 (MQ=255)
                                       cgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGACCg                                                               <  1:2554076/101‑1 (MQ=255)
                                        gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:1590526/1‑101 (MQ=255)
                                        gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:3907924/1‑101 (MQ=255)
                                         acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                              >  2:1200012/6‑101 (MQ=2)
                                          gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.GGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  1:1374645/101‑1 (MQ=255)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                            >  2:3274598/4‑101 (MQ=2)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGaaa                                                            >  1:3976466/4‑101 (MQ=2)
                                            ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  1:3067580/100‑1 (MQ=255)
                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGa                                                         >  1:3171865/1‑101 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActg                                                         <  2:1205994/101‑3 (MQ=2)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgt                                                        >  1:2790/1‑98 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                      >  2:2042619/1‑101 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                      >  1:2868377/1‑101 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                      <  2:2553470/101‑1 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                       <  2:195485/101‑5 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                       <  2:3831300/101‑5 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                       >  2:734698/1‑97 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                      <  2:1451528/101‑6 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                   <  2:1680624/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  1:661760/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                  <  2:3414283/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCATTtcggc                                                  >  2:1336621/1‑99 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   <  2:3746239/101‑9 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  1:861629/1‑93 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   >  1:123665/1‑93 (MQ=2)
                                                    ccccccGAGGGGGCGTGGATCGAAACAGCTTTC.GATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                   <  1:1042252/101‑9 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAA.TGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                  >  1:2423335/1‑92 (MQ=2)
                                                         cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                             <  2:2676829/101‑1 (MQ=255)
                                                         cgcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                             <  1:3634106/101‑1 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                            >  1:974813/1‑101 (MQ=255)
                                                           ggggggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                           <  1:2772321/100‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                          >  2:1290947/1‑101 (MQ=255)
                                                                gcctGGATCGCAAGGGGTATC.G.TGGGACTGGGCAGCCGCCAGCGCCAAGACCATCCCCCCGCGGGGGCGTGGATCGAAACGATTTCTTCGCTTACGACGcc                                      <  1:4429354/98‑1 (MQ=21)
                                                                gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                      >  1:3414102/1‑101 (MQ=255)
                                                                gCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                      >  1:3762495/1‑101 (MQ=255)
                                                                 cGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                     >  1:3843048/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    >  2:272369/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                    >  2:1644636/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGATGCCTAt                                   >  1:744049/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                   >  2:2357160/1‑101 (MQ=255)
                                                                   gggATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                   <  1:1744821/100‑1 (MQ=255)
                                                                    ggATCGAAACTGCTTTC.G.TGGGACTGGCCAGGCGCCCGCGCCAAGCCGCTCCCCCCGCGGGGGCGGGGGTCCGAACCACTTCGTCGGTTCCGCCGGCTATc                                  >  2:546779/1‑101 (MQ=255)
                                                                        cGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTTGCTTACGACGCCTATCTGAt                              >  2:4274464/1‑101 (MQ=255)
                                                                         gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTCTCTGAtt                             >  1:2069828/1‑101 (MQ=255)
                                                                         gAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                             >  1:2195027/1‑101 (MQ=255)
                                                                          aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                            <  1:2131021/101‑1 (MQ=255)
                                                                          aaaCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                            <  1:3284458/101‑1 (MQ=255)
                                                                            aCTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                          >  1:1278164/1‑101 (MQ=255)
                                                                               gCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                       >  1:1900834/1‑101 (MQ=255)
                                                                               gCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                       >  2:1116845/1‑101 (MQ=255)
                                                                                 tttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                     <  1:567083/101‑1 (MQ=255)
                                                                                 tttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCg                     <  1:3491890/101‑1 (MQ=255)
                                                                                  ttC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                    >  1:788801/1‑101 (MQ=255)
                                                                                    c.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTNNGACGCCTATCTGATTTCGGGTCGCTc                  >  1:3361325/1‑101 (MQ=255)
                                                                                    c.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc                  >  1:1967909/1‑101 (MQ=255)
                                                                                      g.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                 >  2:3441424/1‑101 (MQ=255)
                                                                                         gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc               >  2:2966089/1‑101 (MQ=255)
                                                                                          ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTCCGACGCCTATCTGATTTCGGGTCGCTccccc              >  2:4439625/1‑101 (MQ=255)
                                                                                          ggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc              <  2:2288794/101‑1 (MQ=255)
                                                                                            aCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg            >  2:2241332/1‑101 (MQ=255)
                                                                                              tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg          >  1:353638/1‑101 (MQ=255)
                                                                                               ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTTGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg         <  1:1089474/101‑1 (MQ=255)
                                                                                               ggCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg         >  2:1746660/1‑101 (MQ=255)
                                                                                                 ccAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg       >  2:1002776/1‑101 (MQ=255)
                                                                                                  cAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggggg      >  2:3185802/1‑101 (MQ=255)
                                                                                                   aGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc     <  2:788719/101‑1 (MQ=255)
                                                                                                   aGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc     <  1:830751/101‑1 (MQ=255)
                                                                                                    gccgccCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCg    <  1:2351278/101‑1 (MQ=255)
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GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.G.TGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1396461‑1396661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG