Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,820 A→T 13.1% A234A (GCA→GGT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8200A→T13.1% 98.5 ‑1.8 ‑0.0290A234A (GCA→GCTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/24):  ref base (127/125):  total (141/149)
Fisher's exact test strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 309 total aligned reads displayed.

GGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1396720‑1396920
                                                                                                       |                                                                                                    
gggggTGAGGGTAGAAGCCCTGTCCGTCATATGCTGCCACTATCCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACCGTGTGGAT...GATCATGCa                                                                                                      <  2:3620008/101‑1 (MQ=25)
  gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                                    >  2:4093106/1‑101 (MQ=255)
   ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgcg                                                                                                   >  1:2293098/1‑101 (MQ=255)
    gCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgcgc                                                                                                  <  1:2357310/101‑1 (MQ=255)
     cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGcgcg                                                                                                 >  1:77357/1‑101 (MQ=255)
     cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGcgcg                                                                                                 >  1:4310128/1‑101 (MQ=255)
      gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCgg                                                                                                >  1:2404946/1‑101 (MQ=255)
      gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCgg                                                                                                >  2:3795443/1‑101 (MQ=255)
           tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                           <  1:2043289/101‑1 (MQ=255)
             gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                         >  1:3670757/1‑101 (MQ=255)
              aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTg                                                                                        >  2:4174234/1‑101 (MQ=255)
                aCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTggg                                                                                      <  2:3869557/101‑1 (MQ=255)
                 cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGt                                                                                     <  1:3143465/101‑1 (MQ=255)
                    tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGc                                                                                  >  2:1654119/1‑101 (MQ=255)
                     gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCt                                                                                 <  1:845052/101‑1 (MQ=255)
                      tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATAATGCAGCGCGGACGGCTTTGGTCGCta                                                                                >  2:398058/1‑100 (MQ=255)
                       cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcc                                                                               >  1:2995506/1‑101 (MQ=255)
                       cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCCCGGACGGCTTGGGGCCCTcc                                                                               >  2:3055227/1‑101 (MQ=255)
                        ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                              <  2:2075945/101‑1 (MQ=255)
                        ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                              <  2:4271771/101‑1 (MQ=255)
                          tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                            <  2:1989722/101‑1 (MQ=255)
                           cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                                           <  2:1702851/101‑1 (MQ=255)
                           cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                                           >  2:1275183/1‑101 (MQ=255)
                            atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                          >  2:356023/1‑101 (MQ=255)
                            atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                          >  1:1985100/1‑101 (MQ=255)
                             tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                         >  2:4436102/1‑101 (MQ=255)
                             tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgc                                                                         >  2:4405414/1‑101 (MQ=255)
                                 ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCcggg                                                                     >  2:1382446/1‑101 (MQ=255)
                                    ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCg                                                                  <  1:210200/101‑1 (MQ=255)
                                      aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                                <  2:600801/101‑1 (MQ=255)
                                       cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:67730/101‑1 (MQ=255)
                                        gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:901251/101‑1 (MQ=255)
                                          tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:3420958/101‑1 (MQ=255)
                                           ncnnnnncgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGan                                                             <  1:877479/93‑2 (MQ=2)
                                           gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  2:4387017/1‑101 (MQ=255)
                                           gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:2880597/101‑1 (MQ=255)
                                           gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:1554083/101‑1 (MQ=255)
                                           acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:3226878/93‑1 (MQ=2)
                                            cccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:4264517/1‑101 (MQ=255)
                                            catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:3287465/94‑1 (MQ=2)
                                             ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  1:2640378/101‑1 (MQ=255)
                                              tcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          <  2:3926521/96‑1 (MQ=2)
                                               cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:2506944/97‑1 (MQ=2)
                                               cgccgTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:335210/97‑1 (MQ=2)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                      <  1:2586091/100‑2 (MQ=2)
                                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                    >  2:2128208/1‑101 (MQ=255)
                                                    tCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                    <  1:56397/101‑4 (MQ=2)
                                                    gcGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                    <  2:792784/100‑4 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                 <  2:1238493/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                 <  2:1104035/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                 <  1:513112/101‑1 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                >  1:265208/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                 <  2:2417411/101‑7 (MQ=2)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                 >  1:1833026/1‑95 (MQ=2)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                               >  2:1404912/1‑101 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                              >  2:2834643/1‑101 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                              <  2:2266346/101‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                               >  1:436039/1‑93 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                               <  2:1762535/101‑9 (MQ=2)
                                                          cccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatg                                              <  1:1183609/101‑10 (MQ=2)
                                                            cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                          <  2:733141/101‑1 (MQ=255)
                                                              cgcgGGGGCGTTGATCGAAACCGTGTGCAT...GACCGTGCAGCCCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                        <  2:4153514/101‑1 (MQ=37)
                                                              cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                        >  2:3005093/1‑101 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                        <  1:1706315/101‑1 (MQ=255)
                                                               gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                       >  2:1246753/1‑101 (MQ=255)
                                                               gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                       <  1:1002861/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                      >  1:3609729/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                     >  2:922203/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                     <  1:2830967/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                     <  1:3560505/101‑1 (MQ=255)
                                                                     gCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                                 >  1:2156939/1‑101 (MQ=255)
                                                                       gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                               >  1:3946937/1‑101 (MQ=255)
                                                                         ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGCGATAGACCGCGCGGAcc                             >  2:1488286/1‑101 (MQ=255)
                                                                          gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                            <  1:1432607/101‑1 (MQ=255)
                                                                           aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                           >  1:1680447/1‑101 (MQ=255)
                                                                             cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACCGGTCCAGAGATAGACCGCGCGGAcctcct                         >  1:3834407/1‑101 (MQ=255)
                                                                             cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                         <  2:644768/101‑1 (MQ=255)
                                                                             cGAAACCGGGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                         <  1:2444143/101‑1 (MQ=255)
                                                                              gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                        <  1:981007/101‑1 (MQ=255)
                                                                              gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                        <  2:2346170/101‑1 (MQ=255)
                                                                                aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                      <  1:190378/101‑1 (MQ=255)
                                                                                  ccGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                    <  1:1275276/101‑1 (MQ=255)
                                                                                  ccGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                    <  1:4003682/101‑1 (MQ=255)
                                                                                    gtgtGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt                  <  2:1470574/101‑1 (MQ=255)
                                                                                    gtgtGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCCCCGACgc                  >  1:3036912/1‑100 (MQ=255)
                                                                                     tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                 <  2:353603/101‑1 (MQ=255)
                                                                                     tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                 <  2:4309859/101‑1 (MQ=255)
                                                                                      gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                <  1:2526913/101‑1 (MQ=255)
                                                                                        ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCt              <  1:4427145/101‑1 (MQ=255)
                                                                                          at...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc            >  2:3108681/1‑101 (MQ=255)
                                                                                          at...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc            <  1:343501/101‑1 (MQ=255)
                                                                                           t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc           >  2:1537094/1‑101 (MQ=255)
                                                                                               gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc          <  2:941955/101‑1 (MQ=255)
                                                                                                 tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc        <  1:2944675/101‑1 (MQ=255)
                                                                                                  cATTCAGCGCGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCcg       <  2:3446944/101‑1 (MQ=255)
                                                                                                  cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcg       >  1:3988341/1‑101 (MQ=255)
                                                                                                  cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcg       >  1:4146390/1‑101 (MQ=255)
                                                                                                  cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcg       >  2:1880476/1‑101 (MQ=255)
                                                                                                   aTGCAGCGCGGACGGGTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAGAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcgc      >  1:3755638/1‑101 (MQ=255)
                                                                                                      cAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg   >  2:2705037/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
GGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1396720‑1396920

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG