BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,940	G→T	6.1%	L274L (CTG→TGT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,940	0	G→T	6.1%	42.9 0.0 ‑0.6	359	L274L (CTG→CTT)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (11/11): ref base (175/160): total (186/173)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.73e-01

Only 100 of 389 total aligned reads displayed.


CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040


cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                                <  1:4278828/101‑1 (MQ=255)


 tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACA.GCCT.gc                                                                                                               >  1:2168407/1‑101 (MQ=255)


  ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACCT.gcg                                                                                                              >  2:1878981/1‑101 (MQ=255)


    ccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.Gcgcg                                                                                                            >  1:3510114/1‑101 (MQ=255)


        gcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.Cg                                                                                                      <  2:2367403/101‑1 (MQ=255)


         cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                                     >  1:3963777/1‑101 (MQ=255)


         cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                                     >  1:2841261/1‑101 (MQ=255)


         cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGAGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                                     >  1:3859315/1‑101 (MQ=255)


          gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                    <  2:1961925/101‑1 (MQ=255)


          gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                    >  2:2660244/1‑101 (MQ=255)


          gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                    >  1:2041592/1‑101 (MQ=255)


            gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                                 <  1:1382542/101‑1 (MQ=255)


            gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                                 <  2:314363/101‑1 (MQ=255)


                   gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tc.c                                                                                        <  2:2950353/101‑1 (MQ=255)


                       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.Ct                                                                                  <  1:1473242/101‑1 (MQ=255)


                       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCACACGTCGCCCCCCCCCCCCGGGCGGGGGGGGGGAGA.GCCT.GCGCG.GC.CGCC.GCGGcggcccccc                                                                                      >  1:3562552/1‑93 (MQ=255)


                              ggccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.Gt                                                                        <  2:3609481/99‑1 (MQ=255)


                               tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTc                                                                       <  2:953218/101‑1 (MQ=255)


                                  agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                    <  2:842323/101‑1 (MQ=255)


                                  agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                    >  2:588571/1‑101 (MQ=255)


                                  agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                    >  2:4057899/1‑101 (MQ=255)


                                    agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcc                                                                  >  1:1769928/1‑101 (MQ=255)


                                       tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTccccc                                                               <  1:1212332/101‑1 (MQ=255)


                                        aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcccccc                                                              >  2:3519874/1‑101 (MQ=255)


                                          aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTGGCTGCCCCcgc                                                            >  1:247800/1‑101 (MQ=255)


                                           ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                           <  2:2256627/101‑1 (MQ=255)


                                               gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                       >  1:1047280/1‑101 (MQ=255)


                                                 ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                     <  2:54571/101‑1 (MQ=255)


                                                   aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  2:74058/1‑101 (MQ=255)


                                                    ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:2174672/91‑1 (MQ=0)


                                                    ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:378567/91‑1 (MQ=0)


                                                    ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  1:1816521/91‑1 (MQ=0)


                                                     attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 >  1:4402835/11‑101 (MQ=0)


                                                      tcctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:4221095/101‑1 (MQ=255)


                                                      gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 >  1:100646/10‑101 (MQ=9)


                                                      gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:1722630/92‑1 (MQ=9)


                                                       tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  1:2324378/9‑101 (MQ=2)


                                                       tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:982790/93‑1 (MQ=9)


                                                       cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:3292226/101‑1 (MQ=255)


                                                        cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:2967672/95‑1 (MQ=2)


                                                         tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:938767/1‑101 (MQ=255)


                                                         gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  2:3813060/7‑101 (MQ=2)


                                                         gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:4119223/7‑101 (MQ=2)


                                                         aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                              >  1:1629128/7‑101 (MQ=9)


                                                           ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:4076111/4‑101 (MQ=2)


                                                           aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  2:2402684/1‑101 (MQ=255)


                                                           aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                           >  1:1576087/1‑101 (MQ=255)


                                                            gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  1:3784672/101‑1 (MQ=255)


                                                            gACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  1:1882133/101‑1 (MQ=255)


                                                            actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  1:3241675/4‑101 (MQ=9)


                                                             gcggcGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  1:2006636/97‑1 (MQ=2)


                                                             cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  1:835095/99‑2 (MQ=2)


                                                             aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                         >  1:4441821/1‑101 (MQ=255)


                                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                        >  1:278200/2‑99 (MQ=2)


                                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCCGGGGCGTGGATCGAAACac                                        >  2:4340290/1‑100 (MQ=255)


                                                               gtgccgccccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.TGTGCG.TCTTG.A.ACA..GGAT.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAActa                                        <  2:3557405/95‑3 (MQ=2)


                                                               gTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                        <  1:3605582/101‑3 (MQ=9)


                                                                tCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                      <  2:3879256/101‑1 (MQ=255)


                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                      >  2:2511067/1‑97 (MQ=9)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                     <  1:2622314/101‑6 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TT.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACCTCAGc                                    >  1:2409259/1‑101 (MQ=37)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                    >  2:1767107/1‑101 (MQ=255)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCC.GC.AGCG.AGTGCG.TCTGG.A.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgc                                     <  1:308799/101‑6 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                    >  1:168507/1‑95 (MQ=2)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                     >  2:1732173/1‑96 (MQ=9)


                                                                  ccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                    <  2:2041455/100‑1 (MQ=255)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                      >  1:1117362/1‑101 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcccc                                   >  2:3383085/1‑94 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                  >  1:2740051/1‑101 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                  <  2:14609/101‑1 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTCGATCGAAActcggcct                                   >  2:671292/1‑94 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                 <  2:2841054/101‑1 (MQ=255)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  <  2:3466092/101‑9 (MQ=9)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  >  2:3716943/1‑93 (MQ=9)


                                                                      cccccGGGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                <  1:3739742/101‑1 (MQ=255)


                                                                      cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                   <  1:2297508/101‑4 (MQ=2)


                                                                      cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                   <  2:1328175/101‑4 (MQ=2)


                                                                      cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccccgt                                 >  2:1128457/1‑92 (MQ=2)


                                                                      cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                                >  1:195512/1‑91 (MQ=2)


                                                                       ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                               <  2:4443363/101‑1 (MQ=255)


                                                                        cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                              <  1:502468/101‑1 (MQ=255)


                                                                         ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                <  2:1079132/101‑7 (MQ=0)


                                                                         ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                >  2:1654479/1‑95 (MQ=0)


                                                                               gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                       >  1:3363275/1‑101 (MQ=255)


                                                                                ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                      <  2:1571428/101‑1 (MQ=255)


                                                                                 gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                     >  1:3916595/1‑101 (MQ=255)


                                                                                   gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                   >  2:4202962/1‑101 (MQ=255)


                                                                                    tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                  >  2:2438183/1‑101 (MQ=255)


                                                                                      gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                <  2:4146129/101‑1 (MQ=255)


                                                                                       aTCGAACCA.GCCT.GGGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg               <  1:2104225/101‑1 (MQ=255)


                                                                                        tCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa              >  2:1418349/1‑101 (MQ=255)


                                                                                         cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt             <  1:3005314/101‑1 (MQ=255)


                                                                                           aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt           >  1:133380/1‑101 (MQ=255)


                                                                                           aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt           >  1:3668669/1‑101 (MQ=255)


                                                                                            aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc          >  2:21219/1‑101 (MQ=255)


                                                                                             aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc         <  1:638436/101‑1 (MQ=255)


                                                                                                   cT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc    >  2:3950939/1‑101 (MQ=255)


                                                                                                    t.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  1:55430/101‑1 (MQ=255)


                                                                                                     ggcgcG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  1:3134398/100‑1 (MQ=255)


                                                                                                      gcgcG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc  <  1:74071/101‑1 (MQ=255)


CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG