BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,947	C→G	12.4%	R277G (CGC→GGT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,947	0	C→G	12.4%	90.3 ‑1.7 ‑0.6	369	R277G (CGC→GGC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (25/15): ref base (142/143): total (185/184)
Fisher's exact test strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.69e-01

Only 100 of 386 total aligned reads displayed.


CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047


      ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                                      >  2:1512362/1‑101 (MQ=255)


      ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                                      >  2:3784419/1‑101 (MQ=255)


        cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                                    >  1:14634/1‑101 (MQ=255)


         gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                   <  2:3407159/101‑1 (MQ=255)


         gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                   <  1:1880600/101‑1 (MQ=255)


          tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                                 >  2:3586799/1‑101 (MQ=255)


               cGAAACCGGTCCAGAGATAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.c.c                                                                                          <  1:3064231/101‑1 (MQ=255)


                 aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTg                                                                                        >  2:318769/1‑101 (MQ=255)


                  aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                     >  1:2806305/1‑101 (MQ=255)


                  aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                     >  2:285277/1‑101 (MQ=255)


                   aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..Cg                                                                                    <  1:1370727/101‑1 (MQ=255)


                   aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..Cg                                                                                    <  2:3943762/101‑1 (MQ=255)


                     cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                                  >  1:1571533/1‑101 (MQ=255)


                       gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGCg.gg                                                                               >  1:3764269/1‑100 (MQ=255)


                        tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTc                                                                              <  2:953218/101‑1 (MQ=255)


                           agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                                           >  2:588571/1‑101 (MQ=255)


                            gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                                          <  1:976630/101‑1 (MQ=255)


                             agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcc                                                                         >  1:1769928/1‑101 (MQ=255)


                             agaTAGACCGCGCGGACCTCCTCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcc                                                                         <  1:2754403/101‑1 (MQ=255)


                                tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccccc                                                                      <  2:2610443/101‑1 (MQ=255)


                                  gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                                    <  1:4099176/101‑1 (MQ=255)


                                  gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                                    <  2:244993/101‑1 (MQ=255)


                                   aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTGGCTGCCCCcgc                                                                   >  1:247800/1‑101 (MQ=255)


                                    ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                                  <  2:2256627/101‑1 (MQ=255)


                                    ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                                  >  2:1613305/1‑101 (MQ=255)


                                       cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                               <  1:3551321/101‑1 (MQ=255)


                                        gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                              >  1:1047280/1‑101 (MQ=255)


                                         cgGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GTCCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                             <  2:3854366/101‑1 (MQ=38)


                                         cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                             <  1:1933790/101‑1 (MQ=255)


                                          ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                            <  2:54571/101‑1 (MQ=255)


                                            aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                          >  2:74058/1‑101 (MQ=255)


                                             ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                          <  2:2174672/91‑1 (MQ=0)


                                             ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                          <  1:1816521/91‑1 (MQ=0)


                                              attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:2879910/11‑101 (MQ=0)


                                               gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:3687396/10‑101 (MQ=9)


                                               gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:100646/10‑101 (MQ=9)


                                               gtcaacactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  2:1450330/92‑1 (MQ=9)


                                                tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      >  1:2324378/9‑101 (MQ=2)


                                                cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  2:2408563/101‑1 (MQ=255)


                                                acccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                         <  1:550935/94‑1 (MQ=0)


                                                  tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:1725907/1‑101 (MQ=255)


                                                  tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:938767/1‑101 (MQ=255)


                                                  gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    <  1:3118427/95‑1 (MQ=2)


                                                  aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                                     <  1:3871980/95‑1 (MQ=9)


                                                   cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  2:2737221/1‑101 (MQ=255)


                                                   cAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  2:1903621/101‑1 (MQ=255)


                                                   acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:331521/6‑101 (MQ=9)


                                                    cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:3522513/5‑101 (MQ=2)


                                                    aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:318804/101‑1 (MQ=255)


                                                     ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  2:4063411/98‑1 (MQ=2)


                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                <  1:835095/99‑2 (MQ=2)


                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                >  1:1689437/3‑100 (MQ=2)


                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                >  1:298260/3‑100 (MQ=2)


                                                       tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                >  1:4316482/2‑100 (MQ=9)


                                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                               >  1:2414887/2‑99 (MQ=2)


                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                              <  1:3795696/101‑1 (MQ=255)


                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                              <  1:3408607/101‑4 (MQ=9)


                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                               <  2:2341368/101‑2 (MQ=2)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                            <  1:2622314/101‑6 (MQ=2)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                           >  2:1767107/1‑101 (MQ=255)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                           >  2:1983309/1‑95 (MQ=2)


                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                            <  1:215334/101‑6 (MQ=9)


                                                           ccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                           <  2:2041455/100‑1 (MQ=255)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                             >  1:1117362/1‑101 (MQ=2)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACCa                                             >  2:3707640/1‑101 (MQ=2)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                          >  1:19824/1‑94 (MQ=2)


                                                            cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                           >  2:3795821/1‑95 (MQ=9)


                                                             tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTCGATCGAAActcggcct                                          >  2:671292/1‑94 (MQ=9)


                                                             cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                         <  1:3585781/100‑1 (MQ=255)


                                                              ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccacg                                         >  2:4148970/1‑93 (MQ=0)


                                                               cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                          <  1:2297508/101‑4 (MQ=2)


                                                               cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                          <  2:1328175/101‑4 (MQ=2)


                                                               cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccccgt                                        >  2:1128457/1‑92 (MQ=2)


                                                                ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                      <  2:4443363/101‑1 (MQ=255)


                                                                ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                      >  1:4250044/1‑101 (MQ=255)


                                                                ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                       >  2:3952928/1‑91 (MQ=2)


                                                                 cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                        <  1:1610809/101‑6 (MQ=0)


                                                                  ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                       <  2:1079132/101‑7 (MQ=0)


                                                                    gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                  <  1:4058159/101‑1 (MQ=255)


                                                                     cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.TGCA.TCGGCT.TCCC.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTgg                                 >  1:3950927/1‑101 (MQ=255)


                                                                      gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                                >  1:4154273/1‑101 (MQ=255)


                                                                      gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACATCAGCTCGTCTTTGGt                                >  2:4393506/1‑101 (MQ=255)


                                                                       ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCAGCCCGTCGTTGGTc                               >  1:496151/1‑101 (MQ=255)


                                                                       ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                               <  2:2995285/101‑1 (MQ=255)


                                                                        gggGGTGGATGGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  1:2083712/101‑1 (MQ=255)


                                                                        gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  2:852037/101‑1 (MQ=255)


                                                                            gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                          <  2:3333458/101‑1 (MQ=255)


                                                                             tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                         >  1:1326538/1‑101 (MQ=255)


                                                                                  cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                    <  1:3648629/101‑1 (MQ=255)


                                                                                     aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                 <  2:2391997/101‑1 (MQ=255)


                                                                                     aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                 >  2:21219/1‑101 (MQ=255)


                                                                                      accatctt.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                 <  2:3505768/94‑1 (MQ=255)


                                                                                      aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                <  1:638436/101‑1 (MQ=255)


                                                                                            cT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc           >  1:1664569/1‑101 (MQ=255)


                                                                                            cT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc           <  1:4439897/101‑1 (MQ=255)


                                                                                             t.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt          <  1:2021877/101‑1 (MQ=255)


                                                                                               gcgcG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc         <  1:74071/101‑1 (MQ=255)


                                                                                                 gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc       <  1:1186559/101‑1 (MQ=255)


                                                                                                  cg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc      <  2:4271283/101‑1 (MQ=255)


                                                                                                      c.cGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccccc   >  2:1408120/1‑100 (MQ=255)


CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG