BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,957	T→A	5.0%	F280Y (TTC→CAT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,957	0	T→A	5.0%	36.5 ‑2.4 ‑0.0	357	F280Y (TTC→TAC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (5/13): ref base (169/169): total (174/183)
Fisher's exact test strand distribution p-value = 8.94e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 371 total aligned reads displayed.


TGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396857‑1397057


 ggAGCGAAACCGGTCCAGAGATCGACCGCGCGGACCTCCTCAGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                                           <  2:1749438/101‑1 (MQ=255)


       aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTg                                                                                                  >  2:1212239/1‑101 (MQ=255)


       aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTg                                                                                                  >  2:2104088/1‑101 (MQ=255)


       aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CC.C.CTg                                                                                                  >  1:916672/1‑101 (MQ=255)


        aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..c                                                                                               <  2:195434/101‑1 (MQ=255)


         aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..Cg                                                                                              <  1:1370727/101‑1 (MQ=255)


                cAGAGATAGGCCGCGCGGCCCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGc                                                                                      <  1:2306858/101‑1 (MQ=255)


                 agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                                     <  2:842323/101‑1 (MQ=255)


                 agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCt                                                                                     <  1:1781672/101‑1 (MQ=255)


                  gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTc                                                                                    >  2:55417/1‑101 (MQ=255)


                      tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTccccc                                                                                <  1:1212332/101‑1 (MQ=255)


                      tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTccccc                                                                                >  2:907771/1‑101 (MQ=255)


                       aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcccccc                                                                               >  2:3519874/1‑101 (MQ=255)


                              gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                        >  2:638368/1‑101 (MQ=255)


                              gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                        >  1:1047280/1‑101 (MQ=255)


                               cgGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GTCCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                       <  2:3854366/101‑1 (MQ=38)


                               cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                       <  2:4264248/101‑1 (MQ=255)


                                    ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:3592324/91‑1 (MQ=9)


                                    attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  2:2386524/11‑101 (MQ=0)


                                    attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:2879910/11‑101 (MQ=0)


                                     tcctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:4221095/101‑1 (MQ=255)


                                     gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  2:2125338/10‑101 (MQ=9)


                                     gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:100646/10‑101 (MQ=9)


                                      tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:3612837/93‑1 (MQ=9)


                                      cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:3292226/101‑1 (MQ=255)


                                      ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:2609872/94‑1 (MQ=0)


                                      acccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:550935/94‑1 (MQ=0)


                                       ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:2994627/94‑1 (MQ=2)


                                       ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:1561235/101‑1 (MQ=255)


                                        gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:4119223/7‑101 (MQ=2)


                                        aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:1629128/7‑101 (MQ=9)


                                        aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                                               <  1:3871980/95‑1 (MQ=9)


                                         cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGaa                                                             >  2:3134174/1‑101 (MQ=255)


                                         acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:331521/6‑101 (MQ=9)


                                          aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:2402684/1‑101 (MQ=255)


                                          aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                            >  1:1576087/1‑101 (MQ=255)


                                           tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CCG..CGGC.GTCGCCCCCCCCGCGGGGGCGGGGATCGaaa                                                            >  1:3938666/3‑101 (MQ=2)


                                           gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           >  1:590814/1‑101 (MQ=255)


                                           ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:4063411/98‑1 (MQ=2)


                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACTG.ACCCG.GC...AA.CGGGCTATG.CG.C.TGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                          <  1:3763901/99‑2 (MQ=2)


                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                          <  1:835095/99‑2 (MQ=2)


                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                          <  1:4081188/99‑2 (MQ=2)


                                             tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                          <  2:1330382/100‑2 (MQ=9)


                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                         >  1:278200/2‑99 (MQ=2)


                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                         <  1:1512467/101‑1 (MQ=255)


                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCCGGGGCGTGGATCGAAACac                                                         >  2:4340290/1‑100 (MQ=255)


                                              gtgccgccccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.TGTGCG.TCTTG.A.ACA..GGAT.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAActa                                                         <  2:3557405/95‑3 (MQ=2)


                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                        <  2:2292953/101‑1 (MQ=255)


                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                       >  1:2392147/1‑101 (MQ=255)


                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                       <  1:3233515/101‑5 (MQ=2)


                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                        <  1:3408607/101‑4 (MQ=9)


                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                       >  2:2511067/1‑97 (MQ=9)


                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                       >  2:3791207/1‑97 (MQ=9)


                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGAGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcgg                                                       >  2:694300/1‑97 (MQ=9)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACacc                                                        >  1:2431591/1‑99 (MQ=2)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCC.GC.AGCG.AGTGCG.TCTGG.A.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgc                                                      <  1:308799/101‑6 (MQ=2)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                     >  1:168507/1‑95 (MQ=2)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                     <  1:1729196/101‑7 (MQ=2)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                     <  1:1925187/101‑7 (MQ=2)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                      <  1:215334/101‑6 (MQ=9)


                                                 gCTCCCCCAGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                      <  1:1119349/101‑6 (MQ=0)


                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                     >  1:378606/1‑95 (MQ=9)


                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                     <  2:1071546/101‑7 (MQ=9)


                                                   tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcccc                                                    >  2:3383085/1‑94 (MQ=2)


                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                   >  1:2740051/1‑101 (MQ=255)


                                                    ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                  >  2:1450816/1‑101 (MQ=255)


                                                    ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                  <  2:2841054/101‑1 (MQ=255)


                                                    ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                   <  2:3466092/101‑9 (MQ=9)


                                                      ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                 >  1:3585290/1‑91 (MQ=2)


                                                      ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                 >  2:3952928/1‑91 (MQ=2)


                                                        ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                 >  2:1654479/1‑95 (MQ=0)


                                                        ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                              >  2:504291/1‑101 (MQ=255)


                                                         cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                             <  1:1837398/101‑1 (MQ=255)


                                                          gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                            >  2:2021740/1‑101 (MQ=255)


                                                           tgggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                           >  1:684610/2‑101 (MQ=255)


                                                             ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCAGCCCGTCGTTGGTc                                         >  1:496151/1‑101 (MQ=255)


                                                              gggGGTGGATGGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                        <  1:2083712/101‑1 (MQ=255)


                                                               ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                       <  2:3715183/101‑1 (MQ=255)


                                                               ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                       <  2:2915808/101‑1 (MQ=255)


                                                                 cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGcc                                     >  1:1964785/1‑101 (MQ=255)


                                                                   tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                   >  2:2438183/1‑101 (MQ=255)


                                                                   tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                   >  1:1326538/1‑101 (MQ=255)


                                                                     gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                 <  2:4146129/101‑1 (MQ=255)


                                                                       tCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                               >  2:1418349/1‑101 (MQ=255)


                                                                         gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGTTCAGCCGGACGATg                             >  1:3126841/1‑101 (MQ=255)


                                                                         gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                             >  2:1112564/1‑101 (MQ=255)


                                                                         gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                             >  2:3908200/1‑101 (MQ=255)


                                                                          aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                            >  1:3668669/1‑101 (MQ=255)


                                                                           aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                           <  1:2265870/101‑1 (MQ=255)


                                                                                  cT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                     <  1:4439897/101‑1 (MQ=255)


                                                                                   t.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                    >  2:2231599/1‑101 (MQ=255)


                                                                                    tctgcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                    <  2:1821410/98‑1 (MQ=255)


                                                                                      cgcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc                  <  1:3520122/101‑1 (MQ=255)


                                                                                           gC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc              <  2:2645353/101‑1 (MQ=255)


                                                                                              cGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc            <  1:961855/101‑1 (MQ=255)


                                                                                               gCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg           >  1:3984929/1‑101 (MQ=255)


                                                                                               gCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg           <  2:4441549/101‑1 (MQ=255)


                                                                                                ga.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg          <  1:4370743/100‑1 (MQ=255)


                                                                                                cA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg          >  1:4299051/1‑101 (MQ=255)


                                                                                                      gCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg     <  1:2015138/101‑1 (MQ=255)


TGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396857‑1397057

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG