Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,960 G→T 6.3% R281L (CGC→CTT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9600G→T 6.3% 46.0 0.0 ‑0.2355R281L (CGC→CTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/12):  ref base (161/170):  total (173/182)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.70e-01

Only 100 of 371 total aligned reads displayed.

TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396861‑1397060
                                                                                                         |                                                                                                         
tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.c                                                                                                         >  1:2776605/1‑101 (MQ=255)
  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                      <  1:3012471/101‑1 (MQ=255)
  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                      <  1:1473242/101‑1 (MQ=255)
  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                      >  1:2122624/1‑101 (MQ=255)
    aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                                  >  1:2806305/1‑101 (MQ=255)
    aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                                  >  2:285277/1‑101 (MQ=255)
       cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                                               >  1:1571533/1‑101 (MQ=255)
       cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                                               <  2:2204232/101‑1 (MQ=255)
         ggccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.Gt                                                                                            <  2:3609481/99‑1 (MQ=255)
          tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTc                                                                                           <  1:3273787/101‑1 (MQ=255)
             agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                                                        <  2:842323/101‑1 (MQ=255)
              gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                                                       >  2:55417/1‑101 (MQ=255)
              gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                                                       <  1:976630/101‑1 (MQ=255)
                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccccc                                                                                   >  2:907771/1‑101 (MQ=255)
                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcccccc                                                                                  <  1:1364585/101‑1 (MQ=255)
                    gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                                                 <  2:244993/101‑1 (MQ=255)
                      ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                                               <  2:2256627/101‑1 (MQ=255)
                         cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                                            <  1:3551321/101‑1 (MQ=255)
                          gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                           >  2:638368/1‑101 (MQ=255)
                          gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                           >  1:1047280/1‑101 (MQ=255)
                           cgGCCCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GTCCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          <  2:3854366/101‑1 (MQ=38)
                           cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          <  2:4264248/101‑1 (MQ=255)
                                 ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:3119270/92‑1 (MQ=2)
                                 gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:100646/10‑101 (MQ=9)
                                 gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:1722630/92‑1 (MQ=9)
                                  cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:2124760/1‑101 (MQ=255)
                                  cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:2408563/101‑1 (MQ=255)
                                   ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:2299695/94‑1 (MQ=2)
                                   cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:2967672/95‑1 (MQ=2)
                                   caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  1:3158801/8‑101 (MQ=9)
                                    tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  1:938767/1‑101 (MQ=255)
                                    aacactgTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:3713091/95‑1 (MQ=9)
                                      aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGACCGaaa                                                               >  2:1370923/1‑101 (MQ=255)
                                       ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  2:4063411/98‑1 (MQ=2)
                                        gcggcGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:2006636/97‑1 (MQ=2)
                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             <  1:835095/99‑2 (MQ=2)
                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             <  1:4081188/99‑2 (MQ=2)
                                        aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                             <  1:3091156/101‑1 (MQ=255)
                                        aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                             >  2:2773836/1‑101 (MQ=255)
                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAActa                                                            >  2:3437707/2‑99 (MQ=2)
                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            >  1:2414887/2‑99 (MQ=2)
                                         cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCCGGGGCGTGGATCGAAACac                                                            >  2:4340290/1‑100 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                           <  1:3795696/101‑1 (MQ=255)
                                           tctgtCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                           <  1:512214/97‑4 (MQ=2)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          >  1:2326655/1‑101 (MQ=255)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                          <  2:3379842/101‑5 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  2:2341368/101‑2 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                          >  1:456505/1‑97 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                          >  2:3791207/1‑97 (MQ=9)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGAGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcgg                                                          >  2:694300/1‑97 (MQ=9)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                         >  2:1732173/1‑96 (MQ=9)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                          >  1:1117362/1‑101 (MQ=2)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                       <  1:1292481/101‑8 (MQ=2)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGaacctataccg                                                       >  2:2059702/1‑92 (MQ=2)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                        >  2:2015990/1‑95 (MQ=9)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                      <  2:14609/101‑1 (MQ=255)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                      >  2:2604205/1‑93 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       <  1:3773961/101‑8 (MQ=9)
                                                ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                        <  1:2065473/101‑3 (MQ=2)
                                                 cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                                                    >  1:195512/1‑91 (MQ=2)
                                                  ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                   >  1:4250044/1‑101 (MQ=255)
                                                  ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                    >  2:3952928/1‑91 (MQ=2)
                                                    ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                    <  2:1079132/101‑7 (MQ=0)
                                                    ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                                 >  2:504291/1‑101 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                   <  1:654722/101‑8 (MQ=0)
                                                      gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                               <  1:4058159/101‑1 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.TGCA.TCGGCT.TCCC.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTgg                                              >  1:3950927/1‑101 (MQ=255)
                                                        gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                             >  1:4299578/1‑101 (MQ=255)
                                                        gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                                             >  1:4154273/1‑101 (MQ=255)
                                                         ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                            >  2:1774818/1‑101 (MQ=255)
                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                           <  1:2687169/101‑1 (MQ=255)
                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                           >  1:3363275/1‑101 (MQ=255)
                                                           ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                          <  2:1571428/101‑1 (MQ=255)
                                                           ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                          <  2:3715183/101‑1 (MQ=255)
                                                               tGGATCGAAACA.GCCT.GCGCG.GG.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                      <  1:1917606/101‑1 (MQ=255)
                                                                ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  2:3517067/1‑101 (MQ=255)
                                                                 gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                    <  2:4146129/101‑1 (MQ=255)
                                                                  aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                   >  1:2527941/1‑101 (MQ=255)
                                                                   tCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                                  >  2:1418349/1‑101 (MQ=255)
                                                                    cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 <  1:3648629/101‑1 (MQ=255)
                                                                    cGAAACA.GCCT.GCCCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 <  1:3425929/101‑1 (MQ=255)
                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                >  1:1780540/1‑101 (MQ=255)
                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                >  2:1112564/1‑101 (MQ=255)
                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                >  2:3908200/1‑101 (MQ=255)
                                                                       aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                              <  2:2391997/101‑1 (MQ=255)
                                                                            gCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                          <  1:1672375/101‑1 (MQ=255)
                                                                            gCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                          <  1:4405686/101‑1 (MQ=255)
                                                                              cT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                        >  1:1618273/1‑101 (MQ=255)
                                                                               t.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                       <  1:55430/101‑1 (MQ=255)
                                                                               t.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                       <  1:3587047/101‑1 (MQ=255)
                                                                                   gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCtaga                    >  1:1821529/1‑98 (MQ=255)
                                                                                   gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                    <  1:1186559/101‑1 (MQ=255)
                                                                                           gCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              <  2:4441549/101‑1 (MQ=255)
                                                                                           gCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              <  2:3283173/101‑1 (MQ=255)
                                                                                                cGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg          >  2:3552222/1‑101 (MQ=255)
                                                                                                 ggCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc         >  2:1278797/1‑101 (MQ=255)
                                                                                                   cT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGt       <  1:3874181/101‑1 (MQ=255)
                                                                                                      tCCG.C.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGCgg     <  2:1954031/101‑1 (MQ=255)
                                                                                                      tCCG.C.CTG..CGTG.GACGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCGCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTgg     <  1:2216737/101‑1 (MQ=255)
                                                                                                         g.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  >  1:741393/1‑101 (MQ=255)
                                                                                                         |                                                                                                         
TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396861‑1397060

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG