BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,961	C→T	15.3%	R281R (CGC→CTT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,961	0	C→T	15.3%	147.6 ‑3.6 ‑1.7	355	R281R (CGC→CGT)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/20): ref base (138/160): total (173/182)
Fisher's exact test strand distribution p-value = 2.68e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.86e-02

Only 100 of 374 total aligned reads displayed.


TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396861‑1397061


tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.c                                                                                                          >  1:2776605/1‑101 (MQ=255)


  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                       <  1:3012471/101‑1 (MQ=255)


  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                       >  1:2122624/1‑101 (MQ=255)


  gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                                                       <  1:1473242/101‑1 (MQ=255)


    aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                                   >  2:285277/1‑101 (MQ=255)


       cGGTCCAGAGAGAGACCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                                                <  2:4421564/101‑1 (MQ=255)


         ggccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.Gt                                                                                             <  2:3609481/99‑1 (MQ=255)


            cAGAGATAGGCCGCGCGGCCCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGc                                                                                          <  1:2306858/101‑1 (MQ=255)


             agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                                                         <  2:842323/101‑1 (MQ=255)


             agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                                                         >  2:4057899/1‑101 (MQ=255)


             agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                                                         <  1:1781672/101‑1 (MQ=255)


              gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                                                        >  2:55417/1‑101 (MQ=255)


              gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                                                        <  2:3834154/101‑1 (MQ=255)


                  tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccccc                                                                                    >  2:907771/1‑101 (MQ=255)


                   aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcccccc                                                                                   <  1:1364585/101‑1 (MQ=255)


                    gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                                                  <  2:244993/101‑1 (MQ=255)


                    gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                                                  <  1:4099176/101‑1 (MQ=255)


                      ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcgcg                                                                                <  2:2256627/101‑1 (MQ=255)


                         cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                                             <  1:3551321/101‑1 (MQ=255)


                          gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                            >  2:638368/1‑101 (MQ=255)


                          gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                            >  1:1047280/1‑101 (MQ=255)


                           cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                           <  2:4264248/101‑1 (MQ=255)


                               ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  2:378567/91‑1 (MQ=0)


                               ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  2:2174672/91‑1 (MQ=0)


                                 gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:100646/10‑101 (MQ=9)


                                 gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      <  1:1722630/92‑1 (MQ=9)


                                  ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  1:2609872/94‑1 (MQ=0)


                                   ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:2826252/1‑101 (MQ=255)


                                    tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  >  2:1725907/1‑101 (MQ=255)


                                    tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  >  1:938767/1‑101 (MQ=255)


                                    gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  <  1:3118427/95‑1 (MQ=2)


                                    aacactgTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  1:3713091/95‑1 (MQ=9)


                                     cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 >  2:2737221/1‑101 (MQ=255)


                                      cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GGCGCTCCCCCCGCGGGGGGGTGGATCGaa                                                                 >  2:3530442/5‑101 (MQ=9)


                                      aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:318804/101‑1 (MQ=255)


                                      aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                                >  1:1576087/1‑101 (MQ=255)


                                        gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               <  2:1907003/100‑1 (MQ=2)


                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              <  1:835095/99‑2 (MQ=2)


                                        cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              >  1:298260/3‑100 (MQ=2)


                                        aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                              >  1:4441821/1‑101 (MQ=255)


                                         tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              <  2:1330382/100‑2 (MQ=9)


                                         tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              >  1:4316482/2‑100 (MQ=9)


                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAActa                                                             >  2:3437707/2‑99 (MQ=2)


                                         cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCCGGGGCGTGGATCGAAACac                                                             >  2:4340290/1‑100 (MQ=255)


                                         cGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                             <  2:3509873/101‑1 (MQ=255)


                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                           >  1:2326655/1‑101 (MQ=255)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                           >  1:2728771/1‑97 (MQ=9)


                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGAGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcgg                                                           >  2:694300/1‑97 (MQ=9)


                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACacc                                                            >  1:2431591/1‑99 (MQ=2)


                                             ccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                         <  2:2041455/100‑1 (MQ=255)


                                              cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                           >  1:1117362/1‑101 (MQ=2)


                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                        <  1:1292481/101‑8 (MQ=2)


                                              cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                         <  2:1071546/101‑7 (MQ=9)


                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                       <  2:14609/101‑1 (MQ=255)


                                               tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                        <  1:3773961/101‑8 (MQ=9)


                                                ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                         <  1:2065473/101‑3 (MQ=2)


                                                 cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                      <  2:3233692/101‑10 (MQ=9)


                                                  ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                    >  1:4250044/1‑101 (MQ=255)


                                                  ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                    <  2:2122485/101‑1 (MQ=255)


                                                  ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                     >  2:3952928/1‑91 (MQ=2)


                                                   cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                                   <  1:502468/101‑1 (MQ=255)


                                                    ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                                                  >  2:504291/1‑101 (MQ=255)


                                                     cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                    <  1:654722/101‑8 (MQ=0)


                                                      gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                                <  1:4058159/101‑1 (MQ=255)


                                                       cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.TGCA.TCGGCT.TCCC.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTgg                                               >  1:3950927/1‑101 (MQ=255)


                                                        gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                                              >  1:4154273/1‑101 (MQ=255)


                                                         ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                             <  2:2995285/101‑1 (MQ=255)


                                                         ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                             >  2:1774818/1‑101 (MQ=255)


                                                          gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                            >  1:3363275/1‑101 (MQ=255)


                                                           ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                           <  2:3715183/101‑1 (MQ=255)


                                                           ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                           <  2:1571428/101‑1 (MQ=255)


                                                             cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGcc                                         >  1:1964785/1‑101 (MQ=255)


                                                              gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                        <  2:3333458/101‑1 (MQ=255)


                                                               tGGATCGAAACA.GCCT.GCGCG.GG.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                       <  1:1917606/101‑1 (MQ=255)


                                                                 gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                     <  2:4146129/101‑1 (MQ=255)


                                                                   tCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                                   >  2:1418349/1‑101 (MQ=255)


                                                                    cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                  <  1:3648629/101‑1 (MQ=255)


                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                 >  2:1112564/1‑101 (MQ=255)


                                                                     gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg                                 >  2:3908200/1‑101 (MQ=255)


                                                                       aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                               <  1:2265870/101‑1 (MQ=255)


                                                                            gCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                           <  1:1672375/101‑1 (MQ=255)


                                                                            gCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                           <  1:4405686/101‑1 (MQ=255)


                                                                              cT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                         >  1:1618273/1‑101 (MQ=255)


                                                                               t.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                        <  1:3587047/101‑1 (MQ=255)


                                                                               t.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                        <  1:55430/101‑1 (MQ=255)


                                                                                 gcgcG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                       <  1:74071/101‑1 (MQ=255)


                                                                                 gcgcG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                       <  2:2402747/101‑1 (MQ=255)


                                                                                   gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCtaga                     >  1:1821529/1‑98 (MQ=255)


                                                                                   gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc                     <  1:1186559/101‑1 (MQ=255)


                                                                                          cGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc                <  1:961855/101‑1 (MQ=255)


                                                                                          cGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc                >  2:2324486/1‑101 (MQ=255)


                                                                                             a.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg             <  1:3442362/101‑1 (MQ=255)


                                                                                               tccgCT.TCCG.C.CTG..CGGG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg            <  2:1984971/98‑1 (MQ=255)


                                                                                                cGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg           <  2:2595022/101‑1 (MQ=255)


                                                                                                 ggCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc          >  2:1278797/1‑101 (MQ=255)


                                                                                                  gCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg         <  1:2015138/101‑1 (MQ=255)


                                                                                                   cT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGt        <  1:3874181/101‑1 (MQ=255)


                                                                                                    t.tCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg       >  1:3292269/1‑101 (MQ=255)


                                                                                                      tCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTgg      >  2:2387827/1‑101 (MQ=255)


                                                                                                         g.C.CTG..CGTC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGTGGCGTGGATc   <  1:3851550/101‑1 (MQ=255)


TCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1396861‑1397061

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG