BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,002	T→C	8.9%	I295T (ATC→ACC)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,002	0	T→C	8.9%	52.6 ‑3.9 ‑0.8	281	I295T (ATC→ACC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (18/7): ref base (117/139): total (135/146)
Fisher's exact test strand distribution p-value = 1.95e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.47e-01

Only 100 of 297 total aligned reads displayed.


CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTC  >  NC_014034/1396902‑1397102


cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                           <  1:1512467/101‑1 (MQ=255)


 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                                                          <  2:2292953/101‑1 (MQ=255)


 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                                                          <  1:3795696/101‑1 (MQ=255)


  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         >  1:2392147/1‑101 (MQ=255)


  tCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         <  1:124173/101‑1 (MQ=255)


  tCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         <  2:3879256/101‑1 (MQ=255)


    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTTCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACCTCAGc                                                                                                       >  1:2409259/1‑101 (MQ=37)


    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                                                       >  2:1767107/1‑101 (MQ=255)


    ccTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                                                       <  2:2041455/100‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACCa                                                                                                         >  2:3707640/1‑101 (MQ=2)


       ccccccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                                                                       <  1:2065473/101‑3 (MQ=2)


       ccccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                                                                    <  2:2841054/101‑1 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                                                                    >  2:1450816/1‑101 (MQ=255)


        cccccGGGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.t                                                                                                  <  1:3739742/101‑1 (MQ=255)


        cccccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                                                                                      <  1:2297508/101‑4 (MQ=2)


        cccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.t                                                                                                  <  2:4308950/101‑1 (MQ=255)


         ccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tc                                                                                                 >  1:4250044/1‑101 (MQ=255)


         ccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tc                                                                                                 <  2:4443363/101‑1 (MQ=255)


          cccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                                                                    <  1:1610809/101‑6 (MQ=0)


          cccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tcg                                                                                                <  1:502468/101‑1 (MQ=255)


           ccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcg.tcgt                                                                                               >  2:504291/1‑101 (MQ=255)


            cgcgGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                                                                  <  1:654722/101‑8 (MQ=0)


            cgcgGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGtt                                                                                              <  1:1837398/101‑1 (MQ=255)


               gggggCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGt                                                                                           >  1:4299578/1‑101 (MQ=255)


               gggggCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACATCAGCTCG.TCTTTGGt                                                                                           >  2:4393506/1‑101 (MQ=255)


                 gggCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                         <  2:852037/101‑1 (MQ=255)


                   gCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGc                                                                                       >  1:3916595/1‑101 (MQ=255)


                     gTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                                                     >  1:2003683/1‑101 (MQ=255)


                     gTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                                                     <  2:3333458/101‑1 (MQ=255)


                      tGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                                                                    >  1:1326538/1‑101 (MQ=255)


                       ggATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGa                                                                                   >  2:3517067/1‑101 (MQ=255)


                        gATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAc                                                                                  <  2:4146129/101‑1 (MQ=255)


                         aTCGAACCAGCCTGGGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                <  1:2104225/101‑1 (MQ=255)


                         aTCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                >  1:2527941/1‑101 (MQ=255)


                         aTCCAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                <  1:2017254/101‑1 (MQ=255)


                            gAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGTTCAGCCGGAC.GATg                                                                             >  1:3126841/1‑101 (MQ=255)


                            gAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATg                                                                             >  2:1112564/1‑101 (MQ=255)


                              aaCAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                           >  2:21219/1‑101 (MQ=255)


                                 aGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                     <  2:3988085/101‑1 (MQ=255)


                                    cTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                  <  1:4439897/101‑1 (MQ=255)


                                    cTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                  >  2:3950939/1‑101 (MQ=255)


                                    cTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                  >  1:1664569/1‑101 (MQ=255)


                                     tGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                 <  1:55430/101‑1 (MQ=255)


                                     tGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                 <  1:3587047/101‑1 (MQ=255)


                                      gcgcGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                <  1:74071/101‑1 (MQ=255)


                                       cgcgGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                               <  2:2393494/101‑1 (MQ=255)


                                         cgGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccc                                                             <  2:4271283/101‑1 (MQ=255)


                                           gC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccccc                                                           <  2:2645353/101‑1 (MQ=255)


                                            c.cGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccccc                                                          >  2:1408120/1‑100 (MQ=255)


                                              cGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                         >  2:2324486/1‑101 (MQ=255)


                                              cGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                         <  1:961855/101‑1 (MQ=255)


                                                 a.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                      <  1:3442362/101‑1 (MQ=255)


                                                        ttCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                >  1:3292269/1‑101 (MQ=255)


                                                         tCCGCCTGCGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGCgg                                               <  2:1954031/101‑1 (MQ=255)


                                                            gCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:2156800/101‑1 (MQ=255)


                                                            gCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  1:109352/1‑101 (MQ=255)


                                                            gCCTGCGTC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGTGGCGTGGATc                                            <  1:3851550/101‑1 (MQ=255)


                                                             ccccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1058867/94‑1 (MQ=2)


                                                             ccTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:4400810/101‑1 (MQ=255)


                                                              ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:3022725/9‑101 (MQ=2)


                                                              cTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:1319296/1‑101 (MQ=255)


                                                               cagcggcgccGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:2174215/92‑1 (MQ=0)


                                                                agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  2:3233286/7‑101 (MQ=2)


                                                                  gTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                      >  2:3441620/1‑101 (MQ=255)


                                                                   ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:3619808/4‑101 (MQ=0)


                                                                   ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  1:2796244/4‑101 (MQ=0)


                                                                    g.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                      >  1:4034264/1‑100 (MQ=2)


                                                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      >  2:2581381/2‑100 (MQ=2)


                                                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      >  2:3118203/2‑100 (MQ=2)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                  >  2:672483/1‑99 (MQ=2)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                  >  1:792866/1‑99 (MQ=2)


                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                >  1:354305/1‑101 (MQ=255)


                                                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactga                                 >  2:1884826/1‑95 (MQ=2)


                                                                           tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                              >  1:1377496/1‑101 (MQ=255)


                                                                           cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                              <  2:1618167/100‑1 (MQ=255)


                                                                            ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCTCCCGCGGGGGCGTGGATCGAAACGGcgtaga                               <  1:3955013/101‑6 (MQ=2)


                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagat                              <  2:2975994/101‑7 (MQ=2)


                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                            <  1:1774937/101‑1 (MQ=255)


                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                            <  1:4122592/101‑1 (MQ=255)


                                                                              ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTTAg                           >  1:1947618/1‑101 (MQ=255)


                                                                                 cgcgGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctcg                          <  1:1151181/101‑11 (MQ=0)


                                                                                    gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                     >  1:3338280/1‑101 (MQ=255)


                                                                                      gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                   >  2:1227742/1‑101 (MQ=255)


                                                                                          gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg               <  2:2003554/101‑1 (MQ=255)


                                                                                           tGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa              >  1:3494785/1‑101 (MQ=255)


                                                                                             gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc            <  1:2737411/101‑1 (MQ=255)


                                                                                             gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc            <  2:3764016/101‑1 (MQ=255)


                                                                                             gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc            <  1:2660416/101‑1 (MQ=255)


                                                                                             gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc            >  2:2791176/1‑101 (MQ=255)


                                                                                                 gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt        <  1:285312/101‑1 (MQ=255)


                                                                                                  caaCCTCAGCCCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg       <  2:1294523/100‑1 (MQ=255)


                                                                                                  aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg       >  1:2891123/1‑101 (MQ=255)


                                                                                                   aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt      >  1:1451056/1‑101 (MQ=255)


                                                                                                    acctCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc     <  2:3749134/98‑1 (MQ=255)


                                                                                                    aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc     <  2:1091625/101‑1 (MQ=255)


                                                                                                     cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGTGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    <  2:3854418/101‑1 (MQ=255)


                                                                                                     cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    >  2:1484344/1‑101 (MQ=255)


                                                                                                     cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    <  2:1807302/101‑1 (MQ=255)


                                                                                                     cATCAGCTCG.TCGTTGGTCAGACGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    <  1:1720224/101‑1 (MQ=255)


                                                                                                     cATCAGATCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    >  1:1546307/1‑101 (MQ=255)


CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTC  >  NC_014034/1396902‑1397102

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG