Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,017 T→G 12.0% V300G (GTC→CGA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0170T→G12.0% 69.0 0.0 ‑1.1265V300G (GTC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/16):  ref base (102/117):  total (131/134)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.24e-02

Only 100 of 281 total aligned reads displayed.

GGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396917‑1397117
                                                                                                     |                                                                                                        
 ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTc                                                                                                         <  2:2995285/101‑1 (MQ=255)
 ggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTc                                                                                                         >  2:1774818/1‑101 (MQ=255)
  gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                                        >  1:3363275/1‑101 (MQ=255)
  gggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                                        >  2:3873928/1‑101 (MQ=255)
   ggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAg                                                                                                       <  2:3715183/101‑1 (MQ=255)
      gTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                                                                    <  2:3333458/101‑1 (MQ=255)
      gTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                                                                    >  1:2003683/1‑101 (MQ=255)
      gTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGGCAGCCg                                                                                                    >  1:743041/1‑101 (MQ=255)
       tGGATCGAAACAGCCTGCGCGGGCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                                                                                   <  1:1917606/101‑1 (MQ=255)
        ggATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGa                                                                                                  <  2:613299/101‑1 (MQ=255)
         gATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAc                                                                                                 <  2:4146129/101‑1 (MQ=255)
          aTCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                               >  1:2527941/1‑101 (MQ=255)
          aTCCAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                               <  1:2017254/101‑1 (MQ=255)
           tCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.Ga                                                                                              >  2:1418349/1‑101 (MQ=255)
             gAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGTTCAGCCGGAC.GATg                                                                                            >  1:3126841/1‑101 (MQ=255)
              aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                                           >  1:3668669/1‑101 (MQ=255)
              aaaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                                           >  1:133380/1‑101 (MQ=255)
               aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                          >  2:21219/1‑101 (MQ=255)
               aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                          <  1:2265870/101‑1 (MQ=255)
               aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                          <  2:1101399/101‑1 (MQ=255)
               aaCAGCCTCCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                          <  1:1450923/101‑1 (MQ=255)
                  aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                                    <  2:3988085/101‑1 (MQ=255)
                   gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTc                                                                                   <  1:1672375/101‑1 (MQ=255)
                      tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                                >  2:2231599/1‑101 (MQ=255)
                      tGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCt                                                                                <  1:3587047/101‑1 (MQ=255)
                        cgcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                                              <  1:3520122/101‑1 (MQ=255)
                        cgcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                                              <  2:2393494/101‑1 (MQ=255)
                        cgcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                                              >  2:881510/1‑101 (MQ=255)
                         gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCtaga                                                                             >  1:1821529/1‑98 (MQ=255)
                         gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                                             <  1:1186559/101‑1 (MQ=255)
                          cgGCCGCATCGGCTTCCTCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccc                                                                            <  2:3950673/101‑1 (MQ=255)
                              cGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                                        >  2:2324486/1‑101 (MQ=255)
                               gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                       <  2:4441549/101‑1 (MQ=255)
                                  tccgCTTCCGCCTGCGGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                                    <  2:1984971/98‑1 (MQ=255)
                                  tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                                    <  1:139769/101‑1 (MQ=255)
                                   cGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggggg                                                                   >  2:3552222/1‑101 (MQ=255)
                                       ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                               >  1:3292269/1‑101 (MQ=255)
                                         ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:3645498/101‑1 (MQ=255)
                                           gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:2156800/101‑1 (MQ=255)
                                           gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  1:109352/1‑101 (MQ=255)
                                           atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  1:543418/93‑1 (MQ=2)
                                            gcTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:1488391/100‑1 (MQ=255)
                                            ccccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:1058867/94‑1 (MQ=2)
                                            ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:3022725/9‑101 (MQ=2)
                                            ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4400810/101‑1 (MQ=255)
                                            ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  2:1879371/1‑101 (MQ=255)
                                            ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:3460651/1‑101 (MQ=255)
                                             cTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  2:4317152/101‑1 (MQ=255)
                                             cTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:2124899/101‑1 (MQ=255)
                                              tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  1:79543/101‑1 (MQ=255)
                                              tGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGCGGATCGaa                                                        >  1:2073235/1‑101 (MQ=255)
                                              ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4286534/96‑1 (MQ=2)
                                              agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:280086/7‑101 (MQ=2)
                                                cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                      >  1:1542986/1‑101 (MQ=255)
                                                 gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                     >  2:3441620/1‑101 (MQ=255)
                                                 ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       >  2:3761643/3‑101 (MQ=2)
                                                  tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCCCCCCCCGCGGGGGCGGGGACGGAAAcgc                                                    >  1:1589324/1‑99 (MQ=37)
                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcc                                                  <  1:2402841/101‑1 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                 <  1:330904/101‑3 (MQ=2)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                                 >  2:1942141/1‑96 (MQ=2)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgta                                                <  1:1664551/101‑4 (MQ=2)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                              >  2:2590407/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                               >  1:4413468/1‑97 (MQ=2)
                                                         cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                             <  2:1618167/100‑1 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                            >  1:72494/1‑101 (MQ=255)
                                                          ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                            <  1:1250199/101‑1 (MQ=255)
                                                             cccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                         >  2:1045167/1‑101 (MQ=255)
                                                              ccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                                        <  1:4203223/101‑1 (MQ=255)
                                                                gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGAt                                      <  1:1743880/101‑1 (MQ=255)
                                                                   ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                                   >  1:4025492/1‑101 (MQ=255)
                                                                    gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                                  >  2:1227742/1‑101 (MQ=255)
                                                                    gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGAGCGTGGATCGAAACTGCCGCATTGAGTGATgggg                                  >  1:3828836/1‑101 (MQ=255)
                                                                      gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGACACTGCCGTCTTGAGTGATGGGgcg                                >  2:3798102/1‑101 (MQ=255)
                                                                        gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                              >  2:3229006/1‑101 (MQ=255)
                                                                          ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                            >  1:1957190/1‑101 (MQ=255)
                                                                          ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                            >  2:286099/1‑101 (MQ=255)
                                                                           gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                           <  1:2737411/101‑1 (MQ=255)
                                                                           gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                           <  1:2660416/101‑1 (MQ=255)
                                                                           gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                           >  2:131693/1‑101 (MQ=255)
                                                                           gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                           >  2:2791176/1‑101 (MQ=255)
                                                                             tCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac                         <  2:4298782/101‑1 (MQ=255)
                                                                               gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                       <  1:285312/101‑1 (MQ=255)
                                                                                caaCCTCAGCCCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                      <  2:1294523/100‑1 (MQ=255)
                                                                                aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                      >  1:2891123/1‑101 (MQ=255)
                                                                                aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                      >  2:685601/1‑101 (MQ=255)
                                                                                  aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                    <  1:3908454/101‑1 (MQ=255)
                                                                                   cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                   <  2:1807302/101‑1 (MQ=255)
                                                                                   cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                   >  2:1484344/1‑101 (MQ=255)
                                                                                      cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGCGAGCACATGtcgtcg                >  2:3954661/1‑101 (MQ=255)
                                                                                      cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                <  2:2030585/101‑1 (MQ=255)
                                                                                        gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGCGAGCACATGTCGTCGCt              >  1:4332743/1‑101 (MQ=255)
                                                                                        gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGcc              >  1:3872618/1‑100 (MQ=255)
                                                                                        gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt              <  2:247773/101‑1 (MQ=255)
                                                                                        gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt              <  1:451953/101‑1 (MQ=255)
                                                                                          tcg.tcgTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc            >  2:2652358/1‑101 (MQ=255)
                                                                                              tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc         <  1:2438340/101‑1 (MQ=255)
                                                                                                gtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccccc       >  2:3089334/1‑100 (MQ=255)
                                                                                                gtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccccc       >  2:4219480/1‑100 (MQ=255)
                                                                                                  tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg     >  1:395471/1‑101 (MQ=255)
                                                                                                    gTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCggg   <  1:4092627/101‑1 (MQ=255)
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GGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGG  >  NC_014034/1396917‑1397117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG