Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,144 C→A 5.3% R342R (CGC→CGA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1440C→A 5.3% 20.1 ‑0.3 ‑0.6208R342R (CGC→CGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (93/104):  total (99/109)
Fisher's exact test strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.47e-01

Only 100 of 220 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCC  >  NC_014034/1397046‑1397243
                                                                                                  |                                                                                                    
gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                                    >  1:969810/1‑101 (MQ=255)
gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                                    <  2:3562304/101‑1 (MQ=255)
  gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                  <  2:1575981/101‑1 (MQ=255)
  gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                                  >  1:116526/1‑101 (MQ=255)
   ggggCGTGGGTCGAAAGTGCCGTGTTGAGGGATAGGACGAGAGCAAATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                                 <  2:601057/101‑1 (MQ=25)
   ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                                 <  2:2092827/101‑1 (MQ=255)
   ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCACATCGAGCcacaa                                                                                                 >  2:3992304/1‑100 (MQ=255)
    gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                                                                                                <  2:2879483/101‑1 (MQ=255)
     gatctGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                               <  1:3676804/97‑1 (MQ=255)
       cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                             <  2:796696/101‑1 (MQ=255)
       cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                             <  2:1377399/101‑1 (MQ=255)
       cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                             >  1:361483/1‑101 (MQ=255)
       cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                             >  2:1217962/1‑101 (MQ=255)
       cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCt                                                                                             >  2:502652/1‑101 (MQ=255)
        gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCCCATGTCtt                                                                                            >  1:3587035/1‑101 (MQ=255)
        gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                            <  1:4219741/101‑1 (MQ=255)
         tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCttg                                                                                           >  2:4431824/1‑100 (MQ=255)
         tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                           <  2:3872365/101‑1 (MQ=255)
         tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc                                                                                           >  1:2387629/1‑101 (MQ=255)
          ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTCCAGCTCGCGCCCCATGTCTTCg                                                                                          >  1:440929/1‑101 (MQ=255)
            aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                                        >  1:3181326/1‑101 (MQ=255)
             tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                                       >  1:2278646/1‑101 (MQ=255)
              cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                     >  2:1041795/1‑101 (MQ=255)
              acaaaCGGCCGGCGTGAGTGATCGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                                     <  2:4154012/99‑1 (MQ=37)
               gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGTGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                                    <  2:2278501/101‑1 (MQ=255)
               gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                                    <  2:3963521/101‑1 (MQ=255)
               gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                                    >  1:3499206/1‑101 (MQ=255)
                aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                   <  2:3292037/101‑1 (MQ=255)
                aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                   <  1:3388853/101‑1 (MQ=255)
                aaaCTGCCGTCTTGAGTGAGGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                   <  1:3726351/101‑1 (MQ=255)
                 aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                  <  1:1879495/101‑1 (MQ=255)
                 aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                  <  1:2357319/101‑1 (MQ=255)
                  aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcg                                                                                 >  1:2093487/1‑101 (MQ=255)
                   cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcgc                                                                                <  1:2989759/101‑1 (MQ=255)
                    tGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGcc                                                                               <  2:2739861/101‑1 (MQ=255)
                     gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                              >  1:1927010/1‑101 (MQ=255)
                     gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                              >  1:1763442/1‑101 (MQ=255)
                       cctCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                            <  1:1058924/99‑1 (MQ=255)
                         tCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGc                                                                          <  1:2791382/101‑1 (MQ=255)
                          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                         >  2:1856847/1‑101 (MQ=255)
                           ttGAGTGATGGGGCGCGAGCACATGTCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCCCGGCACATGTCTTCGCCC.GCGCGCCGTCGCTc                                                                        >  1:2213132/1‑101 (MQ=37)
                             gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                      >  1:1326397/1‑101 (MQ=255)
                              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                     >  1:2052936/1‑101 (MQ=255)
                              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                     >  2:1441006/1‑101 (MQ=255)
                              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGGCGCTcccc                                                                     >  2:3703101/1‑101 (MQ=255)
                               gTGATGGGGCGCGAGCACATTTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                    <  2:2454930/101‑1 (MQ=255)
                                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                  >  2:3446785/1‑101 (MQ=255)
                                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                  >  1:3122934/1‑101 (MQ=255)
                                 gATGGGGCGCGAGCACATGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                  <  1:685681/101‑1 (MQ=255)
                                     gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                              <  2:3668416/101‑1 (MQ=255)
                                        cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                           >  2:3163692/1‑101 (MQ=255)
                                        cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                           >  2:1778629/1‑101 (MQ=255)
                                          cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                                         <  1:1278890/101‑1 (MQ=255)
                                           gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCCTCGCTCCCCCCGCGGGGGCGTg                                                        >  2:23810/1‑101 (MQ=255)
                                             gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                      >  1:571794/1‑101 (MQ=255)
                                             gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                      >  2:567185/1‑101 (MQ=255)
                                             gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                      >  1:1497185/1‑101 (MQ=255)
                                             gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGGGGa                                                      >  1:3311889/1‑101 (MQ=255)
                                               ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:956503/94‑1 (MQ=0)
                                               ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    >  1:1566557/8‑101 (MQ=0)
                                                gctgcggtcgGTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  2:1268912/95‑1 (MQ=0)
                                                caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:3875263/101‑1 (MQ=255)
                                                 ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  >  2:4050968/6‑101 (MQ=2)
                                                 ctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  1:2944415/96‑1 (MQ=2)
                                                 aTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  1:1613411/101‑1 (MQ=255)
                                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  2:3326050/5‑101 (MQ=2)
                                                     ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                              >  2:2682295/2‑100 (MQ=2)
                                                       tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                            >  1:2626954/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTc                                          >  1:3287320/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                         <  2:2820622/101‑1 (MQ=255)
                                                           cccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                        <  2:4393965/100‑1 (MQ=255)
                                                           cccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                        <  2:4279300/100‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                       >  1:3043381/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGc                                       >  2:889400/1‑101 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                      <  1:810193/101‑1 (MQ=255)
                                                              ccccGCGTGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                     <  2:3126617/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGccc                                     >  2:1467172/1‑100 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                     <  2:3498966/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                     >  1:2674423/1‑101 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACATCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                     <  2:3034618/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                    <  2:2628007/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                    >  1:2917012/1‑101 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                                    <  1:2762552/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACGTGTCTGCCacga                                  >  1:1988999/1‑101 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                                 >  1:3855427/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGccc                             <  2:1926881/101‑1 (MQ=255)
                                                                          gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa                         >  1:4064221/1‑101 (MQ=255)
                                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAc                        <  1:1032626/101‑1 (MQ=255)
                                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAc                        >  1:1787010/1‑101 (MQ=255)
                                                                             gATCGAAACGTCTAGCTCGCGCCACATGTACTCGACC.GTGCGCCGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACGCGTCTGCCACGACGCCCTCCACCa                      <  2:741313/101‑1 (MQ=37)
                                                                             gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                      <  1:2632400/101‑1 (MQ=255)
                                                                             gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCa                      >  1:4171027/1‑101 (MQ=255)
                                                                                cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                   <  2:240093/101‑1 (MQ=255)
                                                                                cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt                   >  1:1417829/1‑101 (MQ=255)
                                                                                    aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgc               >  1:3424039/1‑101 (MQ=255)
                                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGcc            >  2:1625765/1‑101 (MQ=255)
                                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc         <  2:113481/101‑1 (MQ=255)
                                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTc         <  2:1326300/101‑1 (MQ=255)
                                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCt      <  2:1964656/101‑1 (MQ=255)
                                                                                                 gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc  >  1:544917/1‑101 (MQ=255)
                                                                                                  |                                                                                                    
GCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCC  >  NC_014034/1397046‑1397243

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG