Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,489 C→G 14.5% P457P (CCC→CCG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4890C→G14.5% 86.7 ‑1.9 ‑0.0249P457P (CCC→CCGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/20):  ref base (123/90):  total (139/110)
Fisher's exact test strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.66e-01

Only 100 of 264 total aligned reads displayed.

TGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1397389‑1397589
                                                                                                        |                                                                                                    
tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGccc                                                                                                      >  2:4428161/1‑101 (MQ=255)
 ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCt                                                                                                     <  1:1706401/101‑1 (MQ=255)
  gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTg                                                                                                    <  2:854780/101‑1 (MQ=255)
  gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTg                                                                                                    >  2:1241110/1‑101 (MQ=255)
  gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTg                                                                                                    >  2:1847823/1‑101 (MQ=255)
  gATCGAAACTCTACAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTg                                                                                                    >  1:2416776/1‑101 (MQ=255)
   aTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGc                                                                                                   <  2:147359/101‑1 (MQ=255)
   aTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGc                                                                                                   >  2:1001624/1‑101 (MQ=255)
      gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaca                                                                                                >  1:4227759/1‑100 (MQ=255)
      gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaag                                                                                                >  2:2414787/1‑99 (MQ=255)
       aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                               <  2:2379443/101‑1 (MQ=255)
       aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                               <  2:3224688/101‑1 (MQ=255)
       aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                               >  1:1944626/1‑101 (MQ=255)
        aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaccac                                                                                              >  1:2662322/1‑101 (MQ=255)
         aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCaccacc                                                                                             >  2:2820852/1‑101 (MQ=255)
          ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCg                                                                                            >  2:3684923/1‑101 (MQ=255)
            ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGt                                                                                          >  1:793775/1‑101 (MQ=255)
             ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTc                                                                                         >  1:3923504/1‑101 (MQ=255)
                    gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccc                                                                                  >  1:4310050/1‑101 (MQ=255)
                      tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                                <  1:1262614/101‑1 (MQ=255)
                       atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                               >  1:3359454/1‑101 (MQ=255)
                       atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                               <  2:951813/101‑1 (MQ=255)
                         ggCACAACCGTCCGCGACATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgcg                                                                             <  2:2001456/101‑1 (MQ=255)
                          gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgg                                                                            >  1:1988468/1‑101 (MQ=255)
                            aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACAAC.GCAA.CCGCCCTGCACCAACGGTCGCTCCCCCCGCgggg                                                                          >  2:2570081/1‑101 (MQ=255)
                             aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                                         <  2:572717/101‑1 (MQ=255)
                             aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                                         >  1:1592624/1‑101 (MQ=255)
                             aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGACGCTCCCCCCGCggggg                                                                         >  2:4015492/1‑101 (MQ=255)
                                 cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  2:173220/101‑1 (MQ=255)
                                  gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:4075263/1‑101 (MQ=255)
                                   tCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:2995322/101‑1 (MQ=255)
                                     gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGa                                                                   >  1:3149864/9‑101 (MQ=0)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:599647/101‑1 (MQ=255)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:4258147/101‑1 (MQ=255)
                                     cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:205645/1‑101 (MQ=255)
                                      gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  1:2239037/1‑101 (MQ=255)
                                        gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  1:2821059/101‑1 (MQ=255)
                                        accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATCg                                                                >  1:2096492/6‑101 (MQ=0)
                                          cgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:4257819/4‑101 (MQ=2)
                                           ctGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                                           <  2:3027066/100‑1 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaac                                                         <  1:2550768/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacgg                                                          >  1:273419/1‑99 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcc                                                        >  2:2955363/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                                         <  2:2497584/101‑4 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                                         <  2:3231823/101‑4 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggg                                                         >  2:3565193/1‑98 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtg                                                        >  1:4400119/1‑97 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgc                                                       >  2:1456392/1‑96 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggggt                                                       >  1:1884655/1‑96 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGa                                                     >  1:548051/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                      >  2:1742868/1‑95 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                      <  2:308167/101‑7 (MQ=2)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                    >  2:1136457/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                    >  2:2593525/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                                     <  1:3283707/101‑8 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                   <  2:3923732/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACATCGATTATC.AAATCGGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                    <  1:4436900/101‑9 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                   <  2:2083408/101‑10 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                   <  2:4188379/101‑10 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                   <  1:3561874/101‑10 (MQ=2)
                                                     ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggggtccag                                                   >  2:1393504/1‑92 (MQ=2)
                                                      cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                                  <  1:1909549/101‑11 (MQ=2)
                                                      cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                <  2:3734429/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                               >  1:2391337/1‑101 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                               <  1:3766829/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                              <  1:2809044/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                            <  1:3032414/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcccc                                           >  2:2181596/1‑100 (MQ=255)
                                                           gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                           >  2:4089654/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                           >  2:3353052/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCTA.CCTCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGc                                          <  1:3812464/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACACCG.TCATC.CCCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                        <  2:1960397/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                       >  2:1900110/1‑101 (MQ=255)
                                                                cGTGGATCGAAACACCG.TCATC.ACCAC.GCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATAATGGCCCGCCTCa                                      <  2:2894483/101‑1 (MQ=255)
                                                                cGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                      <  1:3659040/101‑1 (MQ=255)
                                                                  tGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                    >  1:762079/1‑101 (MQ=255)
                                                                   ggATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                   >  1:579157/1‑101 (MQ=255)
                                                                    gATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACAACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                                  >  1:1489945/1‑101 (MQ=255)
                                                                     aTCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTNNNngnc                                 >  1:740612/1‑101 (MQ=255)
                                                                      tCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                                <  1:135578/101‑1 (MQ=255)
                                                                      tCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                                >  1:4275591/1‑101 (MQ=255)
                                                                       cGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                               <  1:1099134/101‑1 (MQ=255)
                                                                          aaCACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt                            >  1:2161093/1‑101 (MQ=255)
                                                                             acCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc                         <  1:889485/101‑1 (MQ=255)
                                                                               cG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                       >  1:165736/1‑101 (MQ=255)
                                                                                g.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                      <  2:3470332/101‑1 (MQ=255)
                                                                                    atc.aCCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                   >  1:1196816/1‑101 (MQ=255)
                                                                                      c.accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                 <  1:1001709/101‑1 (MQ=255)
                                                                                         ccac.GCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc               <  1:182586/101‑1 (MQ=255)
                                                                                         ccac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc               >  2:673613/1‑101 (MQ=255)
                                                                                          cac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc              <  1:1438461/101‑1 (MQ=255)
                                                                                            c.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg            <  2:3296020/101‑1 (MQ=255)
                                                                                              gCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc           >  1:2925137/1‑101 (MQ=255)
                                                                                              gCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc           <  2:81869/101‑1 (MQ=255)
                                                                                               cAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg          >  1:3726973/1‑101 (MQ=255)
                                                                                                 a.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg        >  1:1461733/1‑101 (MQ=255)
                                                                                                       ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACAACCCGATCATGGCCCGCCTCGCCGCCGCGCTCACGTCGCTcccccccccgtgtcgt   >  2:610233/1‑91 (MQ=37)
                                                                                                        cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg  >  2:3158082/1‑101 (MQ=255)
                                                                                                        cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg  >  2:1306909/1‑101 (MQ=255)
                                                                                                        cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg  >  2:1644320/1‑101 (MQ=255)
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TGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1397389‑1397589

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG