Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,495 C→G 15.4% T459T (ACC→TCG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4950C→G15.4% 84.1 ‑1.9 ‑0.6233T459T (ACC→ACGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/20):  ref base (114/82):  total (131/102)
Fisher's exact test strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.35e-01

Only 100 of 264 total aligned reads displayed.

GAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397395‑1397595
                                                                                                        |                                                                                                    
 aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                                     <  2:3224688/101‑1 (MQ=255)
 aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                                     >  1:1944626/1‑101 (MQ=255)
 aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                                     <  2:2379443/101‑1 (MQ=255)
  aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaccac                                                                                                    >  1:2662322/1‑101 (MQ=255)
  aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaccac                                                                                                    <  2:2579823/101‑1 (MQ=255)
  aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaccac                                                                                                    >  2:2550605/1‑101 (MQ=255)
      ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGt                                                                                                <  1:3577461/101‑1 (MQ=255)
      ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCACCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGt                                                                                                >  2:2279656/1‑101 (MQ=255)
       ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTc                                                                                               <  1:874534/101‑1 (MQ=255)
       ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTc                                                                                               >  1:2764568/1‑101 (MQ=255)
           aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                           >  1:760246/1‑101 (MQ=255)
             ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTccc                                                                                         >  2:1822211/1‑101 (MQ=255)
              gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccc                                                                                        >  1:4310050/1‑101 (MQ=255)
               atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TTATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTccccc                                                                                       >  1:1109568/1‑101 (MQ=255)
                tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                                      >  1:516672/1‑101 (MQ=255)
                tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                                      <  1:587710/101‑1 (MQ=255)
                 atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                                     <  1:371322/101‑1 (MQ=255)
                 atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                                     >  1:3359454/1‑101 (MQ=255)
                   ggCACAACCGTCCGCGACATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgcg                                                                                   <  2:2001456/101‑1 (MQ=255)
                    gCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgg                                                                                  >  1:1988468/1‑101 (MQ=255)
                     cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACACCG.TCATC.ACCAC.CCAC.CCGCCCTGCCCCACCGTTCCATCCCCCCGCggg                                                                                 >  1:767102/1‑101 (MQ=37)
                      aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgggg                                                                                >  2:1438354/1‑101 (MQ=255)
                       aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                                               >  1:1592624/1‑101 (MQ=255)
                       aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGACGCTCCCCCCGCggggg                                                                               >  2:4015492/1‑101 (MQ=255)
                             tCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:2995322/101‑1 (MQ=255)
                               cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:205645/1‑101 (MQ=255)
                                gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:3028626/101‑1 (MQ=255)
                                gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:2239037/1‑101 (MQ=255)
                                  gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:2821059/101‑1 (MQ=255)
                                  accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:965237/6‑101 (MQ=2)
                                  accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATCg                                                                      >  1:2096492/6‑101 (MQ=0)
                                   aCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:3922512/101‑1 (MQ=255)
                                    cgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  2:4257819/4‑101 (MQ=2)
                                     aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                                                 >  1:578383/1‑101 (MQ=255)
                                     aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                                                 <  2:3612417/101‑1 (MQ=255)
                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacgg                                                                <  2:2725824/101‑3 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                                               <  2:3231823/101‑4 (MQ=2)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                             >  2:2884051/1‑101 (MQ=255)
                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                                             <  1:4089300/101‑1 (MQ=255)
                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtg                                                              >  1:4400119/1‑97 (MQ=2)
                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                            >  2:3511889/1‑101 (MQ=255)
                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggggt                                                             >  1:1884655/1‑96 (MQ=2)
                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                                            <  2:308167/101‑7 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                                           <  1:4238957/101‑8 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                                           <  1:3283707/101‑8 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                         <  2:3923732/101‑1 (MQ=255)
                                              cccccGCGGGGGCGTGGATCGAAACATCGATTATC.AAATCGGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                          <  1:4436900/101‑9 (MQ=2)
                                               ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                         <  2:2083408/101‑10 (MQ=2)
                                                cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                                        <  1:1909549/101‑11 (MQ=2)
                                                 ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                                     >  1:2391337/1‑101 (MQ=255)
                                                   gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                                   <  2:666211/101‑1 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                  >  2:1728107/1‑101 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                  <  2:2895306/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcccc                                                 >  2:2181596/1‑100 (MQ=255)
                                                     gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                                 >  2:4089654/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                                 >  2:3353052/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                                               <  2:2426866/101‑1 (MQ=255)
                                                       gggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                                               <  2:173657/101‑1 (MQ=255)
                                                        ggCGTGGATCGAAACACCG.TCATC.CCCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                              <  2:1960397/101‑1 (MQ=255)
                                                        ggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                              >  1:2700489/1‑101 (MQ=255)
                                                        ggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                              <  2:1602395/101‑1 (MQ=255)
                                                         gCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                             >  2:1900110/1‑101 (MQ=255)
                                                            tGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                          >  1:1465181/1‑101 (MQ=255)
                                                              gATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                                        <  2:95062/101‑1 (MQ=255)
                                                              gATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACAACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                                        >  1:1489945/1‑101 (MQ=255)
                                                               aTCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                                       >  1:1416367/1‑101 (MQ=255)
                                                                tCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                                      >  1:4275591/1‑101 (MQ=255)
                                                                tCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                                      <  1:3112800/101‑1 (MQ=255)
                                                                  gAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                                    >  2:1233465/1‑101 (MQ=255)
                                                                    aaCACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt                                  >  1:2161093/1‑101 (MQ=255)
                                                                      cacCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGa                                <  1:3182106/101‑1 (MQ=255)
                                                                       cccG.TCATC.ACCAC.GCAA.CCCCCCTGTACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc                               <  1:3737700/100‑1 (MQ=255)
                                                                         cG.TCTTC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACAACCCGACCATGGCCCGCCTCAGGGCCGCGCTGACga                             >  2:712878/1‑100 (MQ=38)
                                                                         cG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                             >  1:165736/1‑101 (MQ=255)
                                                                          g.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                            <  2:3470332/101‑1 (MQ=255)
                                                                              atc.aCCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                         >  1:1196816/1‑101 (MQ=255)
                                                                                c.accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                       >  2:519135/1‑101 (MQ=255)
                                                                                c.accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                       <  1:1001709/101‑1 (MQ=255)
                                                                                  accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc                      >  1:692614/1‑101 (MQ=255)
                                                                                   ccac.GCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc                     <  1:182586/101‑1 (MQ=255)
                                                                                    cac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc                    <  1:131714/101‑1 (MQ=255)
                                                                                     cc.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                   <  1:1448125/100‑1 (MQ=255)
                                                                                     ac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                   >  2:411513/1‑101 (MQ=255)
                                                                                      c.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                  >  2:3685331/1‑101 (MQ=255)
                                                                                      c.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                  <  2:3296020/101‑1 (MQ=255)
                                                                                        gCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                 <  2:81869/101‑1 (MQ=255)
                                                                                           a.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCGCGCTTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg              >  1:3805886/1‑101 (MQ=255)
                                                                                             ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCGTGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg             <  1:3932774/101‑1 (MQ=255)
                                                                                             ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg             >  2:3024173/1‑101 (MQ=255)
                                                                                              cgctcggccccaccGGGCGCTCCCCCCGCGGGCGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTCACGTCGCTCCCCCCGCggggg            <  1:2545808/92‑1 (MQ=37)
                                                                                              cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGTGCCTCGCTGACGTCGCTCCCCCCGCggggg            >  2:1468803/1‑101 (MQ=255)
                                                                                               gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCAGCGGGGGc           <  1:2884259/101‑1 (MQ=255)
                                                                                                  cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg        >  2:3158082/1‑101 (MQ=255)
                                                                                                  cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg        >  2:1306909/1‑101 (MQ=255)
                                                                                                   tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCGGGGGCGTgg       >  1:1708627/1‑101 (MQ=255)
                                                                                                    gccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa      <  2:1368589/98‑1 (MQ=255)
                                                                                                    gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa      >  2:3897863/1‑101 (MQ=255)
                                                                                                     caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt     <  1:3543289/101‑1 (MQ=255)
                                                                                                      accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCGGGGGCGCGGATc    >  2:1134617/1‑101 (MQ=255)
                                                                                                       ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg   >  2:4385880/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
GAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397395‑1397595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG