Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,543 G→A 7.7% M475I (ATG→GAA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5430G→A 7.7% 47.5 ‑1.0 ‑0.0299M475I (ATG→ATARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/13):  ref base (135/123):  total (151/148)
Fisher's exact test strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 321 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCC  >  NC_014034/1397443‑1397643
                                                                                                     |                                                                                                       
cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATg                                                                                                         <  2:3734429/101‑1 (MQ=255)
cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATg                                                                                                         >  1:4280316/1‑101 (MQ=255)
 ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATgg                                                                                                        >  1:2391337/1‑101 (MQ=255)
 ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATgg                                                                                                        <  1:3766829/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc                                                                                                       <  1:2809044/101‑1 (MQ=255)
   gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                     >  1:3400422/1‑101 (MQ=255)
   gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c                                                                                                     <  2:4051855/101‑1 (MQ=255)
    cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                  <  2:2895306/101‑1 (MQ=255)
       gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                               <  2:173657/101‑1 (MQ=255)
        ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                              >  1:81882/1‑101 (MQ=255)
              gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                        >  1:1896198/1‑101 (MQ=255)
               aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTNNNngnc                                                                                       >  1:740612/1‑101 (MQ=255)
               aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgcc                                                                                       >  1:1416367/1‑101 (MQ=255)
                 cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccgc                                                                                     >  2:2087318/1‑101 (MQ=255)
                         cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGt                                                                             >  1:165736/1‑101 (MQ=255)
                             atcaCCACGCAACCGCCCTTCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                         <  1:4428433/101‑1 (MQ=255)
                             atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                         >  1:1196816/1‑101 (MQ=255)
                               caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                       <  1:1001709/101‑1 (MQ=255)
                                 ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccc                                                                     >  2:673613/1‑101 (MQ=255)
                                  cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                    <  1:131714/101‑1 (MQ=255)
                                    cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                  <  2:1955200/101‑1 (MQ=255)
                                     gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                 <  2:3145961/101‑1 (MQ=255)
                                        aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                                                              >  1:1461733/1‑101 (MQ=255)
                                         ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg                                                             <  1:1563843/101‑1 (MQ=255)
                                         ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg                                                             >  2:3024173/1‑101 (MQ=255)
                                           gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCAGCGGGGGc                                                           <  1:2884259/101‑1 (MQ=255)
                                             ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                         >  2:3495317/1‑101 (MQ=255)
                                              cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                        <  2:713338/101‑1 (MQ=255)
                                              cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                        >  2:1306909/1‑101 (MQ=255)
                                               tGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  1:3099114/101‑1 (MQ=255)
                                               tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg                                                       >  1:3976835/1‑101 (MQ=255)
                                               tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg                                                       >  1:318928/1‑101 (MQ=255)
                                               tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCGGGGGCGTgg                                                       >  1:1708627/1‑101 (MQ=255)
                                               tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CAGATCATGGC.C..CGCCTCAGCGCCGCGCTTACGTCGCTCCCCCCGCGCGGGCGTgg                                                       >  2:4115048/1‑101 (MQ=255)
                                                 caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:1468908/101‑1 (MQ=255)
                                                 caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:3543289/101‑1 (MQ=255)
                                                  accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCGGGGGCGCGGATc                                                    >  2:1134617/1‑101 (MQ=255)
                                                   tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    <  2:811664/95‑1 (MQ=0)
                                                   ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  2:2282511/101‑1 (MQ=255)
                                                   ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   >  2:4385880/1‑101 (MQ=255)
                                                   atcgccgTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCGCGCAGATCTAGGT.C..TGCGTGGGCTCCCGGCTG.CGTCGCTCCCCCCGCGGGGGCGTGGacca                                                   >  2:4195558/7‑98 (MQ=2)
                                                    aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGTGGAtgaca                                                 >  2:3612589/6‑97 (MQ=0)
                                                     accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  2:551503/1‑101 (MQ=255)
                                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               <  2:2426995/100‑1 (MQ=2)
                                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                              <  2:2375984/101‑1 (MQ=2)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                            >  1:2233830/1‑101 (MQ=2)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                            >  2:1209741/1‑101 (MQ=255)
                                                           ctcTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                           <  2:1838111/99‑1 (MQ=255)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCCTGGATCCAAAcggggt                                          >  1:2113117/1‑96 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGGGGGGATCGAAAcgggtc                                          >  2:4146648/1‑96 (MQ=0)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                            >  1:4047448/1‑99 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                           <  2:2764378/101‑1 (MQ=255)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                          >  2:609272/1‑99 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACaaaa                                           >  2:1595413/1‑98 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                         <  2:2152850/101‑4 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                          >  2:3279913/1‑97 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                        >  2:3981035/1‑97 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                         >  2:879322/1‑96 (MQ=0)
                                                              cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                        <  1:1595519/100‑5 (MQ=2)
                                                               ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                        <  1:3140497/101‑7 (MQ=0)
                                                               ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                       <  2:1109480/101‑1 (MQ=255)
                                                               ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                       <  1:3512130/101‑1 (MQ=255)
                                                                cccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                     <  2:3531133/101‑11 (MQ=0)
                                                                cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                      <  2:1908414/101‑1 (MQ=255)
                                                                cccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                      <  1:666618/101‑1 (MQ=255)
                                                                 ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                     <  1:57499/101‑8 (MQ=2)
                                                                 ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                     <  2:2407254/101‑1 (MQ=255)
                                                                  cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                    <  2:3311248/101‑9 (MQ=0)
                                                                  cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                    <  1:3603453/101‑1 (MQ=255)
                                                                     gcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                 <  1:679015/101‑1 (MQ=255)
                                                                      cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                >  1:1776753/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                               >  1:2130288/1‑101 (MQ=255)
                                                                        ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                              >  1:579678/1‑101 (MQ=255)
                                                                        ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                              <  2:344711/101‑1 (MQ=255)
                                                                        ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                              <  1:1134708/101‑1 (MQ=255)
                                                                         gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                             <  1:1847947/101‑1 (MQ=255)
                                                                         gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CCCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAAATCCGCGGATCGGCGTgc                             >  1:1087594/1‑100 (MQ=255)
                                                                             gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACATCCGCGGATCGGCGTGAcccg                         <  1:272174/101‑1 (MQ=255)
                                                                             gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                         <  1:3729837/101‑1 (MQ=255)
                                                                             gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                         >  2:2860145/1‑101 (MQ=255)
                                                                             gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                         <  2:2700299/101‑1 (MQ=255)
                                                                               ggATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                       <  1:2979290/101‑1 (MQ=255)
                                                                                gATCGAAACACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                      <  2:504163/101‑1 (MQ=255)
                                                                                 aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                     <  2:4275322/101‑1 (MQ=255)
                                                                                  tCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                    <  2:1416260/101‑1 (MQ=255)
                                                                                   cGAAACACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                   <  2:2193823/101‑1 (MQ=255)
                                                                                    gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                  >  1:2682028/1‑101 (MQ=255)
                                                                                       acacc.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt               <  1:947082/101‑1 (MQ=255)
                                                                                       acacc.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt               <  1:24609/101‑1 (MQ=255)
                                                                                       acaac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt               >  1:4324851/1‑101 (MQ=255)
                                                                                        caac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc              <  2:1676376/101‑1 (MQ=255)
                                                                                         aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg             >  1:4178426/1‑101 (MQ=255)
                                                                                          ac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg            >  2:2951438/1‑101 (MQ=255)
                                                                                           c.ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt           <  1:2279801/101‑1 (MQ=255)
                                                                                             ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc          <  1:3901268/101‑1 (MQ=255)
                                                                                             ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGTc          <  1:1982530/101‑1 (MQ=255)
                                                                                               gATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGc        >  1:2569394/1‑101 (MQ=255)
                                                                                                   aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc    >  2:1997991/1‑101 (MQ=255)
                                                                                                   aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc    >  1:3280277/1‑101 (MQ=255)
                                                                                                   aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc    >  1:2192821/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
CCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCC  >  NC_014034/1397443‑1397643

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG