Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,546 C→G 6.7% A476A (GCC→TAG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5460C→G 6.7% 38.2 ‑0.2 ‑0.0298A476A (GCC→GCGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/9):  ref base (140/138):  total (151/147)
Fisher's exact test strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 322 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGC  >  NC_014034/1397446‑1397646
                                                                                                      |                                                                                                      
  gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..cc                                                                                                    >  2:2181596/1‑100 (MQ=255)
  gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..Cg                                                                                                    >  2:3353052/1‑101 (MQ=255)
  gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..Cg                                                                                                    >  2:1272215/1‑101 (MQ=255)
   ggggCGTGGATCGAAACACCGTCATCACCACGCTACCTCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGc                                                                                                   <  1:3812464/101‑1 (MQ=255)
    gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                  >  2:1144214/1‑101 (MQ=255)
    gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                  <  2:2426866/101‑1 (MQ=255)
     ggCGTGGATCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                 <  2:1960397/101‑1 (MQ=255)
     ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                 <  2:1602395/101‑1 (MQ=255)
       cGTGGATCGAAACACCGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATAATGGC.C..CGCCTCa                                                                                               <  2:2894483/101‑1 (MQ=255)
        gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAg                                                                                              >  1:3284405/1‑101 (MQ=255)
         tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgc                                                                                             >  1:762079/1‑101 (MQ=255)
          ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcg                                                                                            >  1:579157/1‑101 (MQ=255)
           gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                           >  1:1908538/1‑101 (MQ=255)
            aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgcc                                                                                          >  1:1416367/1‑101 (MQ=255)
              cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGcgccgc                                                                                        >  1:2829438/1‑101 (MQ=255)
               gAAACACCGTCATCACCACGCAACCGTCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                       <  1:2693877/101‑1 (MQ=255)
               gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                       >  1:2937446/1‑101 (MQ=255)
                   cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGa                                                                                   <  1:3182106/101‑1 (MQ=255)
                    acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGAc                                                                                  <  1:889485/101‑1 (MQ=255)
                      cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGt                                                                                >  1:165736/1‑101 (MQ=255)
                          atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                            >  1:1196816/1‑101 (MQ=255)
                          atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                            <  1:4340559/101‑1 (MQ=255)
                           tcaCCACGCAACCGCCGTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTc                                                                           <  2:3257767/101‑1 (MQ=255)
                            caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                          <  1:1001709/101‑1 (MQ=255)
                             accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccc                                                                         >  1:692614/1‑101 (MQ=255)
                              ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccc                                                                        >  2:673613/1‑101 (MQ=255)
                               cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                       <  1:131714/101‑1 (MQ=255)
                                 cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                     <  2:3296020/101‑1 (MQ=255)
                                 cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                     >  2:3685331/1‑101 (MQ=255)
                                     aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                                                                 >  1:1461733/1‑101 (MQ=255)
                                      ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg                                                                <  1:1563843/101‑1 (MQ=255)
                                       cgctcggccccaccGGGCGCTCCCCCCGCGGGCGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTCACGTCGCTCCCCCCGCggggg                                                               <  1:2545808/92‑1 (MQ=37)
                                          cccgCACCACCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                            <  1:484493/98‑1 (MQ=255)
                                          ccTGCCCCCCCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                            <  1:67039/101‑1 (MQ=255)
                                           cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                           <  2:713338/101‑1 (MQ=255)
                                           cTGCACCACCGGTCGCTCCCCCCGCGAGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:3733532/101‑1 (MQ=255)
                                            tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCCCGGGGGCGTgg                                                          >  1:1708627/1‑101 (MQ=255)
                                            tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CAGATCATGGC.C..CGCCTCAGCGCCGCGCTTACGTCGCTCCCCCCGCGCGGGCGTgg                                                          >  2:4115048/1‑101 (MQ=255)
                                             gccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  2:1368589/98‑1 (MQ=255)
                                              caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:2238892/101‑1 (MQ=255)
                                              caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:3543289/101‑1 (MQ=255)
                                              caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  1:1468908/101‑1 (MQ=255)
                                                tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:811664/95‑1 (MQ=0)
                                                ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  2:4385880/1‑101 (MQ=255)
                                                atcgccgTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCGCGCAGATCTAGGT.C..TGCGTGGGCTCCCGGCTG.CGTCGCTCCCCCCGCGGGGGCGTGGacca                                                      >  2:4195558/7‑98 (MQ=2)
                                                 caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  2:3510489/1‑101 (MQ=255)
                                                  accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  2:551503/1‑101 (MQ=255)
                                                   ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  1:344746/1‑101 (MQ=255)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 >  2:1719657/1‑101 (MQ=255)
                                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 <  1:647964/101‑1 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                <  2:3456483/101‑1 (MQ=2)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 >  2:2944194/1‑101 (MQ=2)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                              <  2:2295930/101‑5 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCCTGGATCCAAAcggggt                                             >  1:2113117/1‑96 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGGGGGGATCGAAAcgggtc                                             >  2:4146648/1‑96 (MQ=0)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACaaa                                               >  1:2253268/1‑99 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                              >  1:3914254/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                             >  2:609272/1‑99 (MQ=2)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                             >  2:2977803/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                             >  1:2392472/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                             >  1:2597123/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                             >  2:3063328/1‑97 (MQ=2)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                            <  2:1896074/101‑1 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                           >  2:3981035/1‑97 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                            >  2:879322/1‑96 (MQ=0)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                            >  2:2847412/1‑96 (MQ=0)
                                                           cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                           <  1:1595519/100‑5 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                          <  1:3512130/101‑1 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                          <  2:2406715/101‑8 (MQ=0)
                                                              ccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                        <  1:4136213/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                       >  1:2726014/1‑93 (MQ=0)
                                                               cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                       <  1:3603453/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                     <  2:208097/101‑1 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                    <  1:4002692/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                   >  2:1801706/1‑101 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                   >  1:1776753/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                  >  1:2130288/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                  <  1:4184498/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                  >  1:99937/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                 <  1:1134708/101‑1 (MQ=255)
                                                                     ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                 >  2:4069216/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGTGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                <  2:1489840/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                <  1:1847947/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                >  2:3566327/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CCCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAAATCCGCGGATCGGCGTgc                                >  1:1087594/1‑100 (MQ=255)
                                                                       ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                               >  1:4282269/1‑101 (MQ=255)
                                                                         cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccc                             >  2:1122285/1‑101 (MQ=255)
                                                                           tGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                           >  1:3195153/1‑101 (MQ=255)
                                                                             gATCGAAACACC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                         <  2:504163/101‑1 (MQ=255)
                                                                              aTCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                        <  2:4275322/101‑1 (MQ=255)
                                                                                    acaac.CCGATCATGGC.C..CACCTCAGCCCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                  <  2:2129956/101‑1 (MQ=255)
                                                                                     caac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                 >  1:3100135/1‑101 (MQ=255)
                                                                                      aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCCCCCATCg                >  1:3043434/1‑101 (MQ=255)
                                                                                        c.ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt              <  1:2279801/101‑1 (MQ=255)
                                                                                        c.ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt              >  2:1925343/1‑101 (MQ=255)
                                                                                          ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCATCGGTc             <  1:1982530/101‑1 (MQ=255)
                                                                                               cATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc        >  2:2733914/1‑101 (MQ=255)
                                                                                                aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc       >  1:2192821/1‑101 (MQ=255)
                                                                                                  ggC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc     >  2:2459428/1‑101 (MQ=255)
                                                                                                   gC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc    <  2:3805633/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
GCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGC  >  NC_014034/1397446‑1397646

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG