Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,547 C→G 7.4% R477G (CGC→GGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5470C→G 7.4% 42.3 ‑1.0 ‑0.0298R477G (CGC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/13):  ref base (142/134):  total (151/147)
Fisher's exact test strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 319 total aligned reads displayed.

CGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCG  >  NC_014034/1397447‑1397647
                                                                                                        |                                                                                                      
cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..c                                                                                                        <  1:3032414/101‑1 (MQ=255)
 gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGc.c..cc                                                                                                       >  2:2181596/1‑100 (MQ=255)
 gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..Cg                                                                                                       >  2:3353052/1‑101 (MQ=255)
  ggggCGTGGATCGAAACACCGTCATCACCACGCTACCTCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGc                                                                                                      <  1:3812464/101‑1 (MQ=255)
   gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                     >  2:1144214/1‑101 (MQ=255)
   gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGcc                                                                                                     <  2:2426866/101‑1 (MQ=255)
    ggCGTGGATCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                    <  2:1960397/101‑1 (MQ=255)
    ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCt                                                                                                    <  2:1602395/101‑1 (MQ=255)
      cGTGGATCGAAACACCGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATAATGGC.C..CGCCTCa                                                                                                  <  2:2894483/101‑1 (MQ=255)
      cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCa                                                                                                  >  2:484441/1‑101 (MQ=255)
          gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAgcgc                                                                                              >  1:1896198/1‑101 (MQ=255)
              gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                          >  2:1233465/1‑101 (MQ=255)
              gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                          >  1:2937446/1‑101 (MQ=255)
              gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                          >  1:2818027/1‑101 (MQ=255)
                         atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                             >  1:1196816/1‑101 (MQ=255)
                         atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                             <  1:4340559/101‑1 (MQ=255)
                           caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                           <  1:1001709/101‑1 (MQ=255)
                            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                          >  1:692614/1‑101 (MQ=255)
                             ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                         <  1:577133/101‑1 (MQ=255)
                             ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                         >  2:673613/1‑101 (MQ=255)
                              cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                        <  1:131714/101‑1 (MQ=255)
                               acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                       <  1:1941620/101‑1 (MQ=255)
                                cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                      <  2:3296020/101‑1 (MQ=255)
                                  cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                    <  1:2593696/101‑1 (MQ=255)
                                    aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                                  >  1:1461733/1‑101 (MQ=255)
                                     ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                 <  1:1563843/101‑1 (MQ=255)
                                      cgctcggccccaccGGGCGCTCCCCCCGCGGGCGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.CA.CGTCGCTCCCCCCGCggggg                                                                <  1:2545808/92‑1 (MQ=37)
                                      cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGTGCCTCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                >  2:1468803/1‑101 (MQ=255)
                                       gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                               <  2:4260715/101‑1 (MQ=255)
                                         cccgCACCACCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                             <  1:484493/98‑1 (MQ=255)
                                         ccTGCCCCCCCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                             <  1:67039/101‑1 (MQ=255)
                                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                            <  2:713338/101‑1 (MQ=255)
                                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                            >  2:3158082/1‑101 (MQ=255)
                                          cTGCACCACCGGTCGCTCCCCCCGCGAGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:3733532/101‑1 (MQ=255)
                                           tGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:3099114/101‑1 (MQ=255)
                                           tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                           >  1:318928/1‑101 (MQ=255)
                                           tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCCCGGGGGCGTgg                                                           >  1:1708627/1‑101 (MQ=255)
                                           tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CAGATCATGGC.C..CGCCTCAGCGCCGCGCT.TA.CGTCGCTCCCCCCGCGCGGGCGTgg                                                           >  2:4115048/1‑101 (MQ=255)
                                           ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:1741267/91‑1 (MQ=2)
                                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:2238892/101‑1 (MQ=255)
                                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:3543289/101‑1 (MQ=255)
                                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:1468908/101‑1 (MQ=255)
                                              cgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3560021/95‑1 (MQ=0)
                                               tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:811664/95‑1 (MQ=0)
                                               atcgccgTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCGCGCAGATCTAGGT.C..TGCGTGGGCTCCCGGCT.G..CGTCGCTCCCCCCGCGGGGGCGTGGacca                                                       >  2:4195558/7‑98 (MQ=2)
                                                cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  1:1361849/96‑1 (MQ=0)
                                                aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGTGGAtgaca                                                     >  2:3612589/6‑97 (MQ=0)
                                                  ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:344746/1‑101 (MQ=255)
                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  >  2:1719657/1‑101 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 <  2:3456483/101‑1 (MQ=2)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  >  2:2944194/1‑101 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                               <  2:2295930/101‑5 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                >  1:2233830/1‑101 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCCTGGATCCAAAcggggt                                              >  1:2113117/1‑96 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGGGGGGATCGAAAcgggtc                                              >  2:4146648/1‑96 (MQ=0)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                               <  2:2836700/101‑2 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                               >  2:1352936/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                               >  1:3914254/1‑101 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                              >  2:609272/1‑99 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                              >  2:2977803/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                              >  2:3063328/1‑97 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                             <  2:1896074/101‑1 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                           <  2:350176/101‑9 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                            >  2:3981035/1‑97 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                             >  2:879322/1‑96 (MQ=0)
                                                          cccccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                            <  1:1595519/100‑5 (MQ=2)
                                                          cccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                             <  1:1654585/100‑6 (MQ=0)
                                                           ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                            <  1:3140497/101‑7 (MQ=0)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                           <  1:3512130/101‑1 (MQ=255)
                                                            cccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                           <  2:2406715/101‑8 (MQ=0)
                                                              cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                        <  1:3603453/101‑1 (MQ=255)
                                                                cgcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                      <  2:208097/101‑1 (MQ=255)
                                                                 gggggggTGTGGGTGGATTCAAC.CCGATCATCGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGTGGCGTGGATCGAAACATCCGCGCATCGGc                                     <  1:610301/99‑1 (MQ=25)
                                                                 gcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                     >  1:2385005/1‑101 (MQ=255)
                                                                 gcgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                     <  1:4002692/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                    >  1:1776753/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                   >  1:2130288/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGccgg                                  >  2:4378506/1‑98 (MQ=255)
                                                                    ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                  >  1:849278/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                  >  2:4069216/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                  <  1:1134708/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                 <  1:1847947/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACAAC.CCGATCATGGC.C..CCCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAAATCCGCGGATCGGCGTgc                                 >  1:1087594/1‑100 (MQ=255)
                                                                      ggCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                >  1:4282269/1‑101 (MQ=255)
                                                                        cGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccc                              >  2:1122285/1‑101 (MQ=255)
                                                                         gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACATCCGCGGATCGGCGTGAcccg                             <  1:272174/101‑1 (MQ=255)
                                                                         gTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                             <  1:981317/101‑1 (MQ=255)
                                                                              tCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                        <  1:742272/101‑1 (MQ=255)
                                                                               cGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                       <  2:547999/101‑1 (MQ=255)
                                                                                gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                      >  1:2682028/1‑101 (MQ=255)
                                                                                gAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                      >  1:2271771/1‑101 (MQ=255)
                                                                                   acaac.CCGATCATGGC.C..CACCTCAGCCCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                   <  2:2129956/101‑1 (MQ=255)
                                                                                     aac.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                 >  1:4178426/1‑101 (MQ=255)
                                                                                      ac.CCGATCATGGC.C..CGCCTCAGCGCCCCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                <  1:411553/101‑1 (MQ=255)
                                                                                       c.ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt               >  2:1925343/1‑101 (MQ=255)
                                                                                              cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc         >  2:2733914/1‑101 (MQ=255)
                                                                                               aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc        >  1:2192821/1‑101 (MQ=255)
                                                                                               aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc        >  1:3280277/1‑101 (MQ=255)
                                                                                                 ggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc      >  2:2459428/1‑101 (MQ=255)
                                                                                                  gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc     <  2:3805633/101‑1 (MQ=255)
                                                                                                        |                                                                                                      
CGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCG  >  NC_014034/1397447‑1397647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG