Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,556 G→A 14.7% A480T (GCC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5560G→A14.7% 99.2 ‑0.3 0.0279A480T (GCC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (120/118):  total (139/140)
Fisher's exact test strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656
                                                                                                       |                                                                                                      
ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                                        >  1:579157/1‑101 (MQ=255)
   tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                                                     <  1:135578/101‑1 (MQ=255)
   tCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                                                     >  1:4275591/1‑101 (MQ=255)
     gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                                   >  1:2193962/1‑101 (MQ=255)
       aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                                 >  1:1868301/1‑101 (MQ=255)
          acCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.c                                                                                            <  1:889485/101‑1 (MQ=255)
                atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      <  1:4340559/101‑1 (MQ=255)
                 tcaCCACGCAACCGCCGTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTc                                                                                     <  2:3257767/101‑1 (MQ=255)
                  caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                    <  1:1001709/101‑1 (MQ=255)
                  caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                    >  2:519135/1‑101 (MQ=255)
                   accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                   >  1:692614/1‑101 (MQ=255)
                    ccacGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                                  <  1:182586/101‑1 (MQ=255)
                     cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                                 <  1:1438461/101‑1 (MQ=255)
                       cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                               <  2:3296020/101‑1 (MQ=255)
                        gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                              <  2:81869/101‑1 (MQ=255)
                        gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                              >  1:2925137/1‑101 (MQ=255)
                        gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                              <  2:3145961/101‑1 (MQ=255)
                         cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                             <  1:2593696/101‑1 (MQ=255)
                          aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                            <  2:1944497/101‑1 (MQ=255)
                            ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                          >  2:3024173/1‑101 (MQ=255)
                            ccGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                          <  1:1563843/101‑1 (MQ=255)
                             cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGTGCCTCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                         >  2:1468803/1‑101 (MQ=255)
                                cccgCACCACCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                      <  1:484493/98‑1 (MQ=255)
                                ccTGCCCCCCCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                      <  1:67039/101‑1 (MQ=255)
                                ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGACAACCCGATCATGGC.C..CGCCTCGCCGCCGCGCT.CA.CGTCGCTcccccccccgtgtcgt                                                                      >  2:610233/1‑91 (MQ=37)
                                ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                                      >  2:3495317/1‑101 (MQ=255)
                                 cTGCACCACCGGTCGCTCCCCCCGCGAGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  1:3733532/101‑1 (MQ=255)
                                  tGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:3099114/101‑1 (MQ=255)
                                  tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:3976835/1‑101 (MQ=255)
                                  tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:318928/1‑101 (MQ=255)
                                   gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:1121442/101‑1 (MQ=255)
                                    caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:1468908/101‑1 (MQ=255)
                                      ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  2:4385880/1‑101 (MQ=255)
                                         gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGGAAc                                                            >  2:2431248/4‑101 (MQ=0)
                                         ccGGTCGCTCCCCACGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  2:3729584/1‑101 (MQ=255)
                                          tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:3267875/100‑1 (MQ=2)
                                          cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:1272308/101‑1 (MQ=255)
                                          cGGTCGCTCCACCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  1:3849619/1‑101 (MQ=255)
                                          cGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:1144305/101‑1 (MQ=255)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           >  2:1719657/1‑101 (MQ=255)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  1:647964/101‑1 (MQ=255)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  2:2375984/101‑1 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           >  2:2944194/1‑101 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  1:3529240/101‑1 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                         >  2:1209741/1‑101 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGGGGGGATCGAAAcgggtc                                                       >  2:4146648/1‑96 (MQ=0)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        >  2:4238696/1‑100 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         >  1:4047448/1‑99 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                        <  1:1926276/101‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                        <  2:2764378/101‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                        >  2:1352936/1‑101 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGTGGATCGaaaaggggcg                                                      >  1:3567972/1‑94 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACaaaa                                                        >  2:1595413/1‑98 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                        <  1:2569240/101‑4 (MQ=2)
                                               ccTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                      <  2:578656/100‑7 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGaaaaggggcga                                                     >  1:1694057/1‑93 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGATCCCCCCGCGGGGGCGTGGATCGAAAcgggtcga                                                     <  1:2133141/101‑8 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                      <  2:886958/101‑4 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                      <  2:1896074/101‑1 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                    <  2:2252183/101‑9 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcggggcgaa                                                    >  2:1129777/1‑93 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     >  2:3981035/1‑97 (MQ=2)
                                                 cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                    <  1:2115922/100‑9 (MQ=2)
                                                 cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     <  1:1595519/100‑5 (MQ=2)
                                                 cccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                      <  1:1654585/100‑6 (MQ=0)
                                                  ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                     <  1:3140497/101‑7 (MQ=0)
                                                   cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                                  <  2:3531133/101‑11 (MQ=0)
                                                    ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                  <  2:2407254/101‑1 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                 >  1:2726014/1‑93 (MQ=0)
                                                     cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCCGCGGATc                                                 <  1:2422240/101‑1 (MQ=255)
                                                       cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                               <  2:208097/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                            >  1:99937/1‑101 (MQ=255)
                                                           tctgCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                           <  1:235923/98‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                           <  1:2037993/101‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                           <  2:344711/101‑1 (MQ=255)
                                                                gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                      >  2:2860145/1‑101 (MQ=255)
                                                                gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                                      >  2:2631251/1‑100 (MQ=255)
                                                                    aTCGAAACACCCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                  <  1:2268745/101‑1 (MQ=255)
                                                                     tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                 <  1:742272/101‑1 (MQ=255)
                                                                       gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                               >  1:2682028/1‑101 (MQ=255)
                                                                          acaacCCGATCATGGC.C..CACCTCAGCCCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                            <  2:2129956/101‑1 (MQ=255)
                                                                            aacCCGATCATGCC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                          <  2:694784/101‑1 (MQ=255)
                                                                                  aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTCCACCCGCGGGGGCGTGGATCGAAACATCCGAGGATCGGCGTGACACGGCCCGCCCATCGGCCGcc                    >  2:2187801/1‑100 (MQ=37)
                                                                                    cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                  >  2:2733914/1‑101 (MQ=255)
                                                                                    cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                  >  1:3448001/1‑101 (MQ=255)
                                                                                     aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                 >  2:77266/1‑101 (MQ=255)
                                                                                        gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc              <  1:4153308/101‑1 (MQ=255)
                                                                                        gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAGCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc              <  2:4075003/101‑1 (MQ=255)
                                                                                        gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc              <  1:3024394/101‑1 (MQ=255)
                                                                                           c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGGCCCCCCCCCcgc            >  2:3607176/1‑101 (MQ=255)
                                                                                              cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg           >  2:4179400/1‑101 (MQ=255)
                                                                                               gCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg          >  1:4208990/1‑101 (MQ=255)
                                                                                               gCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg          >  2:4358992/1‑101 (MQ=255)
                                                                                                ccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg         <  1:2871891/101‑1 (MQ=255)
                                                                                                 cTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg        <  2:3890911/101‑1 (MQ=255)
                                                                                                  ccAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg       <  2:1988164/100‑1 (MQ=255)
                                                                                                   cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc      <  1:1728218/101‑1 (MQ=255)
                                                                                                   cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc      >  2:2971440/1‑101 (MQ=255)
                                                                                                    aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg     <  1:3685176/101‑1 (MQ=255)
                                                                                                     gcgcCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGt    <  1:1056728/101‑1 (MQ=255)
                                                                                                       |                                                                                                      
GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG