Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,412,228 C→G 7.5% C534S (TGT→TCT)  RCAP_rcc01318 ← outer membrane autotransporter barrel domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,412,2280C→G 7.5% 34.4 ‑2.5 ‑0.2227C534S (TGT→TCT) RCAP_rcc01318outer membrane autotransporter barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (5/12):  ref base (109/100):  total (115/112)
Fisher's exact test strand distribution p-value = 8.18e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.86e-01

Only 100 of 258 total aligned reads displayed.

GCCCGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGT  >  NC_014034/1412128‑1412328
                                                                                                    |                                                                                                    
gCCCGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGac                                                                                                      <  1:1352474/101‑1 (MQ=255)
 cccGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGaca                                                                                                     <  2:1993692/101‑1 (MQ=255)
  ccGCGATCGGCGCGGAGAAGGTCTTTCCCCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGacac                                                                                                    <  2:1466151/101‑1 (MQ=255)
   cgcgATCGGGGGGGAGAGGGTCTTTGCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGAcaca                                                                                                   <  1:3443542/101‑1 (MQ=18)
    gcgATCGGCGGGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAg                                                                                                  <  2:1771929/101‑1 (MQ=255)
    gcgATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGATGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAg                                                                                                  <  2:420092/101‑1 (MQ=255)
     cgATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAgg                                                                                                 >  1:2737221/1‑101 (MQ=255)
     cgATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAgg                                                                                                 >  1:2139775/1‑101 (MQ=255)
       aTCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGcc                                                                                               >  1:159355/1‑101 (MQ=255)
       aTCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGcc                                                                                               <  2:2397969/101‑1 (MQ=255)
        tCGGCGCGGAGAGGGTCTTTCCCCCCGCCAGTGACTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCg                                                                                              <  1:3793225/101‑1 (MQ=18)
            cgcgGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGtctc                                                                                          <  2:1688088/101‑1 (MQ=255)
            cgcgGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGtctc                                                                                          >  1:100004/1‑101 (MQ=255)
              cgGAGAAGGTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTccc                                                                                        <  2:2144447/101‑1 (MQ=255)
                gagaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCcgc                                                                                      <  2:2539949/101‑1 (MQ=255)
                gagaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCcgc                                                                                      >  2:2823719/1‑101 (MQ=255)
                  gaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCACCTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGTgg                                                                                    >  2:303086/1‑101 (MQ=255)
                  gaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCgg                                                                                    >  1:1465180/1‑101 (MQ=255)
                  gaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCgg                                                                                    >  1:1179457/1‑101 (MQ=255)
                  gaAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGAGGTGCCCGCAAGACACAGGCCGTCTCCCGCgg                                                                                    >  2:1269193/1‑101 (MQ=255)
                  gaAGGTCTTCACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCgg                                                                                    <  2:2021943/101‑1 (MQ=255)
                   aaGGTCTTTCCCCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGc                                                                                   <  2:1890073/101‑1 (MQ=255)
                    aGGTTTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCa                                                                                  <  1:2855572/101‑1 (MQ=255)
                     gctctttcgccccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCAt                                                                                 <  2:1080659/91‑1 (MQ=255)
                           tACCCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAggtgg                                                                           <  2:524095/101‑1 (MQ=255)
                            acacccgccCGTGTCTGATCGCCCGAGGGGCGCACCACGGGGCCGTCAACTGCGCAGGGGGGGCCCGCAAAACCCAGGGCGCCTCCCGCGGGCTAgggggg                                                                          >  2:3826379/4‑100 (MQ=16)
                             cgcccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGgtggtg                                                                         <  2:844321/101‑1 (MQ=255)
                             cccccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGgtggtg                                                                         <  1:1567862/99‑1 (MQ=255)
                               cccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGcc                                                                       >  2:4112054/1‑101 (MQ=255)
                               cccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGcc                                                                       <  2:2241305/101‑1 (MQ=255)
                               cccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGcc                                                                       <  2:2345349/101‑1 (MQ=255)
                                ccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGccc                                                                      <  1:3475285/101‑1 (MQ=255)
                                ccgccACTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGccc                                                                      <  1:739620/101‑1 (MQ=255)
                                  gccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCAc                                                                    <  2:334730/101‑1 (MQ=255)
                                   ccAGTGTCTGATCGCCGGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCAcc                                                                   <  2:3448018/101‑1 (MQ=255)
                                    cAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcg                                                                  <  1:2379982/101‑1 (MQ=255)
                                     actgtCTGATCGCCCGAGGTGCGCACCGCGGTGCCGTCAGCTGCGAAGGGGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgc                                                                 <  2:682596/99‑1 (MQ=255)
                                     aGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgc                                                                 <  2:327515/101‑1 (MQ=255)
                                     aGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgc                                                                 <  2:2758111/101‑1 (MQ=255)
                                      gtgtCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgcg                                                                <  1:3362989/101‑1 (MQ=255)
                                      gtgtCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgcg                                                                >  1:3191065/1‑101 (MQ=255)
                                        gtCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGcc                                                              <  2:876946/101‑1 (MQ=255)
                                         tCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGccc                                                             <  2:270600/101‑1 (MQ=255)
                                          cTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcg                                                            <  1:4197486/101‑1 (MQ=255)
                                          cTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcg                                                            <  2:1193295/101‑1 (MQ=255)
                                           tGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcgc                                                           <  1:1874649/101‑1 (MQ=255)
                                            gATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcgcg                                                          >  1:1429630/1‑101 (MQ=255)
                                              tCGCCCGAGGTGCGCGCCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGAc                                                        <  2:3565755/101‑1 (MQ=255)
                                              tCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGAc                                                        >  2:2176548/1‑101 (MQ=255)
                                               cGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACt                                                       <  2:4033104/101‑1 (MQ=255)
                                               cGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACt                                                       >  1:1720759/1‑101 (MQ=255)
                                                gCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACtt                                                      >  2:4412415/1‑101 (MQ=255)
                                                gCCCGACGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTCCCCCCAAGACCCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACtt                                                      <  1:3629212/101‑1 (MQ=17)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     <  1:4257202/101‑1 (MQ=255)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     <  1:3844721/101‑1 (MQ=255)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     <  2:2335112/101‑1 (MQ=255)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     <  1:3655616/101‑1 (MQ=255)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     >  2:1155089/1‑101 (MQ=255)
                                                    gAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGc                                                  >  1:2262462/1‑101 (MQ=255)
                                                     aGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCg                                                 >  2:4250568/1‑101 (MQ=255)
                                                          cgcTCCCCGGTGCCGTGAACTGGGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTTCCCACCGCGCCCGCGACTTGGGCGTCAAc                                            <  1:2340062/101‑1 (MQ=14)
                                                          cgcACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCaaa                                            >  1:3606218/1‑100 (MQ=255)
                                                           gcACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACa                                           >  2:3200145/1‑101 (MQ=255)
                                                           gcACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACa                                           >  1:3557772/1‑101 (MQ=255)
                                                             accacGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATa                                         >  1:2647947/1‑101 (MQ=255)
                                                               cacGGGGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAgg                                       <  2:95387/101‑1 (MQ=255)
                                                                 cGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                     <  1:3914196/101‑1 (MQ=255)
                                                                 cGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                     >  1:1650159/1‑101 (MQ=255)
                                                                  ggTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCt                                    >  1:4133786/1‑101 (MQ=255)
                                                                  ggTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCt                                    >  1:571173/1‑101 (MQ=255)
                                                                   gTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                   <  2:2526187/101‑1 (MQ=255)
                                                                    tcccGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                  <  1:1499538/99‑1 (MQ=255)
                                                                    tGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                  <  1:1753170/101‑1 (MQ=255)
                                                                     ggcGTCAACTGCGAAGGTGGTCCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCg                                 <  2:3629832/99‑1 (MQ=255)
                                                                       cggcAACTGCGAAGGTGGTGCCCGTAAGACACAGGCCGTCTTCCGCGGCATAGGTGGTGCCCACCGCGTCCGCGTCTTGGGCTTCATCATAGGCCtttggg                               <  2:1851011/98‑5 (MQ=14)
                                                                       ccccAACTGCGAAGATGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGTATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAg                               <  2:2956250/98‑1 (MQ=255)
                                                                        gTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAgg                              <  1:3082201/101‑1 (MQ=255)
                                                                          cAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                            <  2:2372168/101‑1 (MQ=255)
                                                                           aaCTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCa                           >  1:48261/1‑101 (MQ=255)
                                                                            aCTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCAc                          >  2:390812/1‑101 (MQ=255)
                                                                               gCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTg                       <  1:2513059/101‑1 (MQ=9)
                                                                                 gAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGc                     <  1:4435154/101‑1 (MQ=255)
                                                                                 gAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGc                     <  2:1316450/101‑1 (MQ=9)
                                                                                  aaagtggtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGcc                    <  2:2578230/98‑1 (MQ=2)
                                                                                   aGGTGGTGCCCGCAAGACACAGGCCGCCTCCCGCGGCATAGGTGGTGCCCGCTGCGCCCGCGACTTGGGCGTCATCATATCCCTTCGCGGCCACCTGGccc                   >  2:1489926/1‑101 (MQ=14)
                                                                                    ggtggtGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCg                  >  2:1408036/1‑101 (MQ=40)
                                                                                     gtggtgCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGc                 >  2:18065/1‑101 (MQ=40)
                                                                                      tggtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGca                >  1:4007005/1‑100 (MQ=11)
                                                                                       ggtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGcaa               >  1:749240/1‑99 (MQ=11)
                                                                                             cGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcg         >  1:632263/1‑101 (MQ=255)
                                                                                              gCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcgg        >  1:4355409/1‑101 (MQ=255)
                                                                                                aaGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTg      >  2:556802/1‑101 (MQ=255)
                                                                                                 aGACCCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGc     <  2:159334/101‑1 (MQ=255)
                                                                                                 aGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCCCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGc     <  1:2188537/101‑1 (MQ=255)
                                                                                                 aGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGc     >  1:3463969/1‑101 (MQ=255)
                                                                                                 aGACACAGGCCGGCTCCCGCGGCATAGGTGGTGCCCCCCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGc     <  1:1930922/101‑1 (MQ=255)
                                                                                                  gACCCAGGCCGCCCCCCGCGGCATAGGTGGGGCCCACCGCTCCCGCGCCTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGcc    <  2:3151764/101‑1 (MQ=17)
                                                                                                    cacaGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGt  >  2:602274/1‑101 (MQ=255)
                                                                                                    cacaGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGt  >  1:554561/1‑101 (MQ=255)
                                                                                                    cacaGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGGGCCgg  >  2:386741/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
GCCCGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGT  >  NC_014034/1412128‑1412328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG