Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,389 C→A 16.9% intergenic (‑168/‑790) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3890C→A16.9% 99.5 0.0 ‑0.0259intergenic (‑168/‑790)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (20/24):  ref base (100/115):  total (120/139)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 275 total aligned reads displayed.

TATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCC  >  NC_014034/1434289‑1434488
                                                                                                      |                                                                                                    
tatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGc                                                                                                      >  2:1063157/1‑101 (MQ=255)
  tGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctc                                                                                                    <  1:2610751/101‑1 (MQ=255)
   gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                   >  1:3320352/1‑101 (MQ=255)
   gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                   <  2:161923/101‑1 (MQ=255)
    cACTGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAC..GTCTCCGCTCTg                                                                                                  >  2:1167739/1‑101 (MQ=255)
     aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                                 <  2:1319897/101‑1 (MQ=255)
     aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                                 >  2:195163/1‑101 (MQ=255)
        gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                              <  2:3594681/101‑1 (MQ=255)
          ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                            <  2:3908332/101‑1 (MQ=255)
           tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                           >  2:3943652/1‑101 (MQ=255)
            aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                         <  2:3177324/101‑1 (MQ=255)
            aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                         >  2:945568/1‑101 (MQ=255)
              cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCt                                                                                       >  1:3832469/1‑101 (MQ=255)
               gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                      <  2:3018653/101‑1 (MQ=255)
               gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                      >  2:3104359/1‑101 (MQ=255)
                tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGc                                                                                     <  1:4354252/101‑1 (MQ=255)
                  gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGt                                                                                   >  1:4382445/1‑101 (MQ=255)
                   aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                  <  1:998849/101‑1 (MQ=255)
                   aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                  <  2:4371506/101‑1 (MQ=255)
                   aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTc                                                                                  <  1:3004494/101‑1 (MQ=255)
                    tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCa                                                                                 >  1:2122402/1‑101 (MQ=255)
                    tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCa                                                                                 <  1:2043447/101‑1 (MQ=255)
                     cGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                                >  2:1929464/1‑101 (MQ=255)
                      gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAc                                                                               >  2:4402081/1‑101 (MQ=255)
                         aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                            <  2:2802041/101‑1 (MQ=255)
                         aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                            <  1:3229817/101‑1 (MQ=255)
                             gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCg                                                                        >  2:58299/1‑101 (MQ=255)
                               tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCt                                                                      <  1:776547/101‑1 (MQ=255)
                                 ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcc                                                                    <  1:2384602/101‑1 (MQ=255)
                                  tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccc                                                                   <  1:1368388/101‑1 (MQ=255)
                                   ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccc                                                                  >  1:837354/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                 >  1:4232647/1‑101 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                 <  2:2329403/101‑1 (MQ=255)
                                    tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                 <  1:2505289/101‑1 (MQ=255)
                                     gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccccc                                                                <  2:3328091/101‑1 (MQ=255)
                                        cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgcg                                                             <  2:35022/101‑1 (MQ=255)
                                        cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgcg                                                             >  2:2971574/1‑101 (MQ=255)
                                         ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                            >  2:2538669/1‑101 (MQ=255)
                                              acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                       >  2:836379/1‑101 (MQ=255)
                                                cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                     >  2:1876360/1‑101 (MQ=255)
                                                  gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  1:3985240/1‑101 (MQ=255)
                                                  gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTAGCTCCCCCCGCGGGGGCGTGGa                                                   >  2:1412933/1‑101 (MQ=255)
                                                   aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  >  1:2914540/1‑101 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:4070240/101‑1 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:1530816/101‑1 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:676489/101‑1 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:3174238/101‑1 (MQ=255)
                                                    tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  1:1848825/101‑1 (MQ=255)
                                                    ggggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                                    <  1:4326248/92‑1 (MQ=2)
                                                     gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  2:4286213/9‑101 (MQ=2)
                                                     gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  2:1722519/9‑101 (MQ=2)
                                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                <  1:415222/96‑1 (MQ=2)
                                                        ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  1:3131705/6‑101 (MQ=2)
                                                         tatcggcGCCCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               <  2:6921/95‑1 (MQ=2)
                                                          atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  2:2112980/98‑1 (MQ=2)
                                                          atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              >  2:92064/4‑101 (MQ=2)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                         >  1:2567861/1‑101 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                        >  1:244714/1‑101 (MQ=255)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                          >  1:3909243/1‑99 (MQ=2)
                                                               ctcTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                      <  2:328003/99‑1 (MQ=255)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                         <  1:1963134/101‑4 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                        <  1:2619103/101‑5 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                     >  1:610430/1‑101 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                       <  2:1234944/101‑6 (MQ=2)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                       <  2:3879206/101‑6 (MQ=2)
                                                                    cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                    <  1:3674730/101‑9 (MQ=2)
                                                                    cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                 <  1:719478/101‑1 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                 <  2:4002554/101‑1 (MQ=255)
                                                                    cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                 <  2:1798100/101‑1 (MQ=255)
                                                                      cccGGGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                               <  2:527727/101‑1 (MQ=255)
                                                                      cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                  <  1:542129/101‑11 (MQ=0)
                                                                      cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacgctgctgaca                                  >  2:3253945/1‑91 (MQ=0)
                                                                      cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                               <  1:1671018/101‑1 (MQ=255)
                                                                       ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                              <  2:3745297/101‑1 (MQ=255)
                                                                       ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                              >  1:4056004/1‑101 (MQ=255)
                                                                         gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                            >  1:112119/1‑101 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                           <  2:3516399/101‑1 (MQ=255)
                                                                          cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                           >  1:916345/1‑101 (MQ=255)
                                                                             gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCACGGATTGCATGGCGCCGAg                        <  1:3292247/101‑1 (MQ=255)
                                                                             gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                        <  1:3460559/101‑1 (MQ=255)
                                                                               gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                      >  2:2250551/1‑101 (MQ=255)
                                                                                cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                     <  1:1063245/101‑1 (MQ=255)
                                                                                cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                     <  1:2729583/101‑1 (MQ=255)
                                                                                 gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                    <  1:2250696/101‑1 (MQ=255)
                                                                                 gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                    >  1:2175813/1‑101 (MQ=255)
                                                                                  tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAcc                   >  2:2911092/1‑100 (MQ=255)
                                                                                  tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                   <  2:1548467/101‑1 (MQ=255)
                                                                                   ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                  <  2:742140/101‑1 (MQ=255)
                                                                                   ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                  >  1:1921602/1‑101 (MQ=255)
                                                                                     aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACaaga                >  2:3405299/1‑98 (MQ=255)
                                                                                        gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt             >  1:3403590/1‑101 (MQ=255)
                                                                                         aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg            >  1:102042/1‑101 (MQ=255)
                                                                                          aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc           <  2:4083459/101‑1 (MQ=255)
                                                                                          aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc           >  2:4228079/1‑101 (MQ=255)
                                                                                               gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGTGCCGAGGGACAGGCTTTGCAgg        >  2:2425139/1‑100 (MQ=255)
                                                                                               gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTACCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTCCAGt        >  2:2637283/1‑101 (MQ=255)
                                                                                                tctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc       <  1:1630704/101‑1 (MQ=255)
                                                                                                tctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc       <  1:1737767/101‑1 (MQ=255)
                                                                                                   ccGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt    <  2:2474479/101‑1 (MQ=255)
                                                                                                     gCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc  <  1:1264735/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
TATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCC  >  NC_014034/1434289‑1434488

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG