BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,434,397	C→G	15.7%	intergenic (‑176/‑782)	RCAP_rcc01334 ← / → RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,434,397	0	C→G	15.7%	101.3 ‑0.1 0.0	267	intergenic (‑176/‑782)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand): new base (19/23): ref base (105/119): total (124/143)
Fisher's exact test strand distribution p-value = 8.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.


GCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGG  >  NC_014034/1434297‑1434497


 cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCa                                                                                                         <  2:2395705/101‑1 (MQ=255)


  ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                                        >  2:719398/1‑101 (MQ=255)


   tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                                       >  1:4371777/1‑101 (MQ=255)


    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                                     <  2:3177324/101‑1 (MQ=255)


    aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                                     >  2:945568/1‑101 (MQ=255)


      cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.Ct                                                                                                  >  1:4335384/1‑101 (MQ=255)


       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                                                 >  2:3104359/1‑101 (MQ=255)


       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                                                 <  2:3018653/101‑1 (MQ=255)


       gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                                                 >  2:1348755/1‑101 (MQ=255)


           aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTc                                                                                             <  2:4371506/101‑1 (MQ=255)


            tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                                            >  1:2122402/1‑101 (MQ=255)


            acGAAACAGTCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                                            <  2:4243873/100‑1 (MQ=255)


              gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAc                                                                                          >  2:4402081/1‑101 (MQ=255)


               aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAcc                                                                                         >  1:1873161/1‑101 (MQ=255)


                aaCAGGCTGCTTTGGGCGGAACACCTCATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCt                                                                                        <  1:1783265/101‑1 (MQ=255)


                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                       <  2:2802041/101‑1 (MQ=255)


                 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                       <  1:4157542/101‑1 (MQ=255)


                  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGGCAACCTT..g                                                                                    >  2:3697759/1‑101 (MQ=255)


                   aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..Gt                                                                                   <  1:3854351/101‑1 (MQ=255)


                   aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..Gt                                                                                   <  2:590703/101‑1 (MQ=255)


                        gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTc                                                                              >  1:2663583/1‑101 (MQ=255)


                         ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTcc                                                                             <  1:2384602/101‑1 (MQ=255)


                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccc                                                                            <  1:1368388/101‑1 (MQ=255)


                          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccc                                                                            <  1:2054931/101‑1 (MQ=255)


                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccccc                                                                          <  1:2505289/101‑1 (MQ=255)


                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccccc                                                                          <  2:2329403/101‑1 (MQ=255)


                            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTccccc                                                                          >  1:4232647/1‑101 (MQ=255)


                             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTcccccc                                                                         <  2:3328091/101‑1 (MQ=255)


                             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTcccccc                                                                         <  1:4138672/101‑1 (MQ=255)


                              ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCcg                                                                        <  2:1017068/101‑1 (MQ=255)


                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                                     >  2:2908993/1‑101 (MQ=255)


                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                                     >  2:2538669/1‑101 (MQ=255)


                                 ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCgg                                                                     <  2:556004/101‑1 (MQ=255)


                                  gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCCT..GTCCCTCCCCCCCCggg                                                                    >  2:1936292/1‑101 (MQ=255)


                                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                >  2:836379/1‑101 (MQ=255)


                                       ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                               <  1:3943906/101‑1 (MQ=255)


                                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTg                                                              >  2:1876360/1‑101 (MQ=255)


                                         tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCCGGGGCGGgg                                                             >  1:1651916/1‑101 (MQ=255)


                                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  1:3985240/1‑101 (MQ=255)


                                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  1:3725152/1‑101 (MQ=255)


                                           aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:3056009/101‑1 (MQ=255)


                                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:676489/101‑1 (MQ=255)


                                             gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  2:1722519/9‑101 (MQ=2)


                                             gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  1:3412312/9‑101 (MQ=2)


                                             gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  2:4286213/9‑101 (MQ=2)


                                             gggctatcgTCGCCCCGCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  2:3494524/93‑1 (MQ=2)


                                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:3548482/94‑1 (MQ=2)


                                               tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:2122263/101‑1 (MQ=255)


                                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:171081/95‑1 (MQ=2)


                                                gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:2172817/101‑1 (MQ=255)


                                                ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:2200877/96‑1 (MQ=2)


                                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  1:3205421/101‑1 (MQ=255)


                                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                    <  1:153926/101‑1 (MQ=255)


                                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:92064/4‑101 (MQ=2)


                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                  >  1:2567861/1‑101 (MQ=255)


                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                    >  2:3512317/1‑100 (MQ=2)


                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                  <  1:3255038/101‑4 (MQ=2)


                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                              >  1:610430/1‑101 (MQ=255)


                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                <  1:2454522/101‑6 (MQ=2)


                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                <  2:3879206/101‑6 (MQ=2)


                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctca                                                >  2:1420907/1‑96 (MQ=2)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                            <  1:697272/101‑1 (MQ=255)


                                                           ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                           <  2:1808225/101‑1 (MQ=255)


                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                          >  1:2911328/1‑101 (MQ=255)


                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                          >  2:3925210/1‑101 (MQ=255)


                                                            cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                          <  1:2610500/101‑1 (MQ=255)


                                                             ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.G.C.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                          <  1:887402/101‑8 (MQ=37)


                                                             ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                         <  1:763554/101‑1 (MQ=255)


                                                             ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GCCGCTCCCCCCGCGTGGGGGCGGTTCTAAAAACGGAGTGCCTg                                         >  2:3566317/1‑101 (MQ=37)


                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                           <  1:542129/101‑11 (MQ=0)


                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                           >  1:475795/1‑91 (MQ=0)


                                                              cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGGGGATCGAAacgctgctgaca                                           >  2:3253945/1‑91 (MQ=0)


                                                              cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                        <  1:1968487/101‑1 (MQ=255)


                                                               ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                       >  1:4056004/1‑101 (MQ=255)


                                                                 gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                     >  1:112119/1‑101 (MQ=255)


                                                                   gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                   >  1:945224/1‑101 (MQ=255)


                                                                    ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                                  <  2:1616658/101‑1 (MQ=255)


                                                                     gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                 >  1:830453/1‑101 (MQ=255)


                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                              <  1:1063245/101‑1 (MQ=255)


                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                              <  2:2050301/101‑1 (MQ=255)


                                                                        cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                              <  2:3984145/101‑1 (MQ=255)


                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                             <  1:2250696/101‑1 (MQ=255)


                                                                         gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                             <  2:1205490/101‑1 (MQ=255)


                                                                               cGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                       >  2:677295/1‑101 (MQ=255)


                                                                               cGAAAC..CGCCCCGGTCTTGTTCCCG.GCCTGCGCCACCCTT..GT.GCCCCCCCCCGGGGGGGGGGGGTCGAAACCCGGATTGCATGGCGCCGAGGGACAGGCtt                       <  1:1929593/101‑1 (MQ=2)


                                                                                gAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                      >  1:3403590/1‑101 (MQ=255)


                                                                                 aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttc                     >  1:3135624/1‑100 (MQ=255)


                                                                                 aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                     >  1:102042/1‑101 (MQ=255)


                                                                                  aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                    <  2:4083459/101‑1 (MQ=255)


                                                                                       gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                 <  2:3298033/101‑1 (MQ=255)


                                                                                       gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTACCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTCCAGt                 >  2:2637283/1‑101 (MQ=255)


                                                                                        tctcCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGCATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                >  1:3304569/1‑101 (MQ=255)


                                                                                         ctcCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg               <  2:2935751/101‑1 (MQ=255)


                                                                                          tcCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc              >  1:1628774/1‑101 (MQ=255)


                                                                                           ccGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt             <  2:2474479/101‑1 (MQ=255)


                                                                                             gCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc           >  2:3746879/1‑101 (MQ=255)


                                                                                             gCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc           <  1:1264735/101‑1 (MQ=255)


                                                                                                 tGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc       <  2:2171747/101‑1 (MQ=255)


                                                                                                  gCTTCACG.G.CTGCGTCAGCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg      >  1:2936188/1‑101 (MQ=255)


                                                                                                     tCACG.G.CTGCGTCACCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg   <  2:2971584/101‑1 (MQ=255)


GCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGG  >  NC_014034/1434297‑1434497

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG