Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,412 C→G 16.1% intergenic (‑191/‑767) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4120C→G16.1% 102.5 0.0 ‑0.0261intergenic (‑191/‑767)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (97/121):  total (116/145)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.64e-01

Only 100 of 279 total aligned reads displayed.

AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1434312‑1434509
                                                                                                        |                                                                                                 
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                                 <  1:3229817/101‑1 (MQ=255)
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                                 <  1:4157542/101‑1 (MQ=255)
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCtt                                                                                                 <  1:2277029/101‑1 (MQ=255)
   cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGGCAACCTTg                                                                                                >  2:3697759/1‑101 (MQ=255)
    aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGt                                                                                               <  2:590703/101‑1 (MQ=255)
     ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTc                                                                                              <  2:3124079/101‑1 (MQ=255)
           tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccc                                                                                        >  1:3490295/1‑101 (MQ=255)
            ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTcccc                                                                                       <  2:92199/101‑1 (MQ=255)
             tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccccc                                                                                      >  1:4232647/1‑101 (MQ=255)
             tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccccc                                                                                      <  1:2505289/101‑1 (MQ=255)
              gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTcccccc                                                                                     <  1:4138672/101‑1 (MQ=255)
                 cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcgcg                                                                                  <  2:35022/101‑1 (MQ=255)
                 cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcgcg                                                                                  >  2:2971574/1‑101 (MQ=255)
                 cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcgcg                                                                                  >  2:173786/1‑101 (MQ=255)
                  ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                                                 <  2:556004/101‑1 (MQ=255)
                       acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                                            <  2:1412701/101‑1 (MQ=255)
                        ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                                                           <  1:3943906/101‑1 (MQ=255)
                         cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                          >  2:1876360/1‑101 (MQ=255)
                          tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:2497195/101‑1 (MQ=255)
                          tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCCGGGGCGGgg                                                                         >  1:1651916/1‑101 (MQ=255)
                            aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  1:2914540/1‑101 (MQ=255)
                            aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:566512/101‑1 (MQ=255)
                            aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:3056009/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:3174238/101‑1 (MQ=255)
                               ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:3561209/8‑101 (MQ=2)
                                tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  1:2879387/1‑101 (MQ=255)
                                gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:171081/95‑1 (MQ=2)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:940045/101‑1 (MQ=255)
                                 ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  2:4305926/6‑101 (MQ=2)
                                  tatcggcGCCCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:6921/95‑1 (MQ=2)
                                  tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  1:1860024/97‑1 (MQ=2)
                                   tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                <  1:153926/101‑1 (MQ=255)
                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                              >  1:2567861/1‑101 (MQ=255)
                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                 <  2:3394029/100‑1 (MQ=2)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                             <  1:928347/101‑1 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                                             >  2:2135634/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                           <  1:694375/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                             <  1:2619103/101‑5 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                            <  2:1234944/101‑6 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                            <  1:2454522/101‑6 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctca                                                            >  2:1420907/1‑96 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                           <  2:864078/101‑7 (MQ=2)
                                             cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                      <  2:4002554/101‑1 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                      >  2:3925210/1‑101 (MQ=255)
                                              ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                        <  2:3796003/101‑10 (MQ=2)
                                               cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacgctgctgaca                                                       >  2:3253945/1‑91 (MQ=0)
                                               cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                    <  1:1968487/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                   <  2:3745297/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                   <  2:4382173/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                   >  2:2927204/1‑101 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                                 >  1:112119/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                >  1:916345/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCACGGATTGCATGGCGCCGAg                                             <  1:3292247/101‑1 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                             >  1:830453/1‑101 (MQ=255)
                                                        gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                           >  2:2250551/1‑101 (MQ=255)
                                                         cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                          <  1:1063245/101‑1 (MQ=255)
                                                          gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                         >  2:1508196/1‑101 (MQ=255)
                                                          gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                         >  1:2175813/1‑101 (MQ=255)
                                                           tctaTCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                        <  1:3405536/98‑1 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                       >  2:3045302/1‑101 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                       <  2:742140/101‑1 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                       <  2:2354906/101‑1 (MQ=255)
                                                              aTCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACaaga                                     >  2:3405299/1‑98 (MQ=255)
                                                                cGAAAC..CGCCCCGGTCTTGTTCCCG.GCCTGCGCCACCCTTGT.GCCCCCCCCCGGGGGGGGGGGGTCGAAACCCGGATTGCATGGCGCCGAGGGACAGGCtt                                   <  1:1929593/101‑1 (MQ=2)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtttc                                 >  1:3135624/1‑100 (MQ=255)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                 >  1:102042/1‑101 (MQ=255)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                 <  2:111768/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                <  2:1945817/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                <  2:4083459/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                >  1:768777/1‑101 (MQ=255)
                                                                        gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                             >  2:4259189/1‑101 (MQ=255)
                                                                        gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                             >  1:3355969/1‑101 (MQ=255)
                                                                        gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                             <  2:3298033/101‑1 (MQ=255)
                                                                         tctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                            <  1:1630704/101‑1 (MQ=255)
                                                                          ctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                           >  1:4018680/1‑101 (MQ=255)
                                                                              gCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                       <  1:1264735/101‑1 (MQ=255)
                                                                              gCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcc                       <  1:4378314/101‑1 (MQ=255)
                                                                                tctGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                     >  1:2063454/1‑101 (MQ=255)
                                                                                tctGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                     <  1:173807/101‑1 (MQ=255)
                                                                                tctGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                     >  1:389516/1‑101 (MQ=255)
                                                                                 ctGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                    >  1:361596/1‑101 (MQ=255)
                                                                                  tGCTTCACG.G.CTGCGTCAATCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                   <  2:2063317/101‑1 (MQ=255)
                                                                                  tGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                   <  1:2240912/101‑1 (MQ=255)
                                                                                  tGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                   <  1:659365/101‑1 (MQ=255)
                                                                                   gCTTCACG.G.CTGCGTCAGCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                  >  1:2936188/1‑101 (MQ=255)
                                                                                   gCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                  <  1:514680/101‑1 (MQ=255)
                                                                                   gCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACACGCTTTGCAGTCGCTtcccccc                  >  2:1833928/1‑100 (MQ=255)
                                                                                    cTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc                 >  2:2876943/1‑101 (MQ=255)
                                                                                    cTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc                 >  1:2145233/1‑101 (MQ=255)
                                                                                       cACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCTCGCggg              >  1:731717/1‑101 (MQ=255)
                                                                                          g.g.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc           <  1:2701357/101‑1 (MQ=40)
                                                                                          g.g.CGGCGTCAACCTTGGCCCCCCCCCGGGGGGGGCGTGGACCGAAACACGGATTGCTGGGCGCGGAGGGACGGGCTTTGCATTCGCCCCCCCCGCGGGGGc           <  2:3203405/101‑1 (MQ=9)
                                                                                              cTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGt         <  2:2700581/101‑1 (MQ=39)
                                                                                              cTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGt         <  2:2567698/101‑1 (MQ=39)
                                                                                                gCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg       <  1:244035/101‑1 (MQ=37)
                                                                                                gCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg       <  2:1628668/101‑1 (MQ=37)
                                                                                                  gTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGAt     >  1:3732222/1‑101 (MQ=34)
                                                                                                  gTCAACCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTAGAt     <  1:1413040/101‑1 (MQ=19)
                                                                                                   tCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc    >  1:1327370/1‑101 (MQ=33)
                                                                                                     aaCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  2:2432292/101‑1 (MQ=31)
                                                                                                        |                                                                                                 
AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1434312‑1434509

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG