Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,398 G→A 3.0% intergenic (+76/‑285) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,3980G→A 3.0% 2.7 ‑1.2 ‑0.8131intergenic (+76/‑285)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (83/44):  total (87/44)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.55e-01

Only 100 of 172 total aligned reads displayed.

CAACCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACC  >  NC_014034/1612298‑1612498
                                                                                                    |                                                                                                       
cAACCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCag                                                                                                         >  1:1388744/1‑101 (MQ=255)
cAACCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCag                                                                                                         <  1:2534586/101‑1 (MQ=255)
 aaCCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCaga                                                                                                        <  1:3226253/101‑1 (MQ=255)
 aaCCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCaga                                                                                                        >  2:1002045/1‑101 (MQ=255)
   ccGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAgg                                                                                                      <  2:3604423/101‑1 (MQ=255)
   ccGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAgg                                                                                                      >  1:1868506/1‑101 (MQ=255)
    cGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGc                                                                                                     <  1:3103084/101‑1 (MQ=255)
     gCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGcc                                                                                                    <  2:3478612/101‑1 (MQ=255)
     ccAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGcc                                                                                                    <  2:1861929/100‑1 (MQ=255)
      cAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGccc                                                                                                   <  2:1885208/101‑1 (MQ=255)
      cAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGccc                                                                                                   <  2:215045/101‑1 (MQ=255)
      cAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGccc                                                                                                   >  2:1664519/1‑101 (MQ=255)
       aGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCt                                                                                                  <  2:1721955/101‑1 (MQ=255)
          cGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.Cgg                                                                                              <  2:300498/101‑1 (MQ=255)
           gTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGc                                                                                             >  2:3480075/1‑101 (MQ=255)
           gTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACAGTGGCCCCGCGACCGCCGAGTGCCCAGAGGCCCT.CGGc                                                                                             >  2:2821171/1‑101 (MQ=255)
            tCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGGCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGcc                                                                                            >  1:973886/1‑101 (MQ=255)
             cTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGCCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCt                                                                                           >  1:1385441/1‑101 (MQ=255)
               ggATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGTCCT.CGGCCTgc                                                                                         >  2:420303/1‑101 (MQ=255)
               ggATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTgc                                                                                         <  1:1620262/101‑1 (MQ=255)
                gATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTgcg                                                                                        <  2:323749/101‑1 (MQ=255)
                 aTCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTgcgc                                                                                       <  1:2998351/101‑1 (MQ=255)
                 aTCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTgcgc                                                                                       >  1:3948161/1‑101 (MQ=255)
                   cTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGcgccc                                                                                     >  2:914250/1‑101 (MQ=255)
                    tgtgACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGcgcccg                                                                                    >  2:3558405/1‑101 (MQ=255)
                    tgcgACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGCCCTGcgcccg                                                                                    >  1:721692/4‑101 (MQ=255)
                       gACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCgcccgccc                                                                                 <  2:1283450/101‑1 (MQ=255)
                       gACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCgcccgccc                                                                                 <  2:4070128/101‑1 (MQ=255)
                        aCGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGcccgcccc                                                                                >  1:1568959/1‑101 (MQ=255)
                         cGGCGACCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGcccgccccg                                                                               <  2:3892836/101‑1 (MQ=255)
                         cGGCGACCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGcccgccccg                                                                               <  1:4219467/101‑1 (MQ=255)
                           gCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCccgccccgcc                                                                             >  1:2946804/1‑101 (MQ=255)
                            cgctCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCcgccccgccc                                                                            <  2:1066464/98‑1 (MQ=255)
                            cGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCcgccccgccc                                                                            <  2:3022834/101‑1 (MQ=255)
                             gacccGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCa                                                                           <  1:2623324/98‑1 (MQ=255)
                             gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCa                                                                           >  1:1481699/1‑101 (MQ=255)
                             gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCa                                                                           >  2:2696745/1‑101 (MQ=255)
                             gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCa                                                                           >  2:927364/1‑101 (MQ=255)
                              aTCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCAt                                                                          >  1:3857788/1‑101 (MQ=255)
                              aTCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCCCCCAt                                                                          >  1:4233613/1‑101 (MQ=255)
                                ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATAGGCCCCGTGACCGCCGAGGACCCCGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATgg                                                                        >  1:3812550/1‑101 (MQ=255)
                                ccGCGGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATgg                                                                        <  2:3947907/101‑1 (MQ=255)
                                 cGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATggc                                                                       <  1:3770684/101‑1 (MQ=255)
                                   cTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATggcgg                                                                     >  2:3823731/1‑101 (MQ=255)
                                     gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATggcgggg                                                                   >  2:1974884/1‑100 (MQ=255)
                                       tCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGgcgc                                                                 >  1:2700434/1‑101 (MQ=255)
                                       tCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGgcgc                                                                 >  1:833895/1‑101 (MQ=255)
                                          gACCGGGGCCAGCGCCGAGGCCACGCGCCACATTGGCCCCGTGCCCGCCGGGGGCCCAGGGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAtt                                                              <  1:1713168/101‑1 (MQ=14)
                                          gACCGGGGCCACCGCCGAGGCCACGCGACCCATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAtt                                                              <  1:2218400/101‑1 (MQ=17)
                                          gACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGgggccct                                                              >  1:1294922/1‑98 (MQ=255)
                                           gccgggcccATCGCCGAGGTCACGCGACACATGGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                             <  2:3637159/94‑1 (MQ=17)
                                           cccGAGGCCAGCGCCGGGGTCCCGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGGGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                             <  1:3464977/100‑1 (MQ=17)
                                              gAGGCCATCGCGGAGGTCACGCGACACATTGCCCCCGGGACCGCCGAGGCCCCAGGGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTcgcg                                                          <  1:4326204/101‑1 (MQ=17)
                                                    gtCGCCGAGCTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.ccgccg                                                   <  2:4266771/100‑1 (MQ=255)
                                                     tCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCg.ccgcccg                                                  >  2:4114973/1‑99 (MQ=255)
                                                      cGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGACCTGCGCCCGCCCCGCCCATGGCGGGGGAGATCGCG.CCGCCc.gg                                                >  2:2845578/1‑101 (MQ=17)
                                                      cGCCGAGGCCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCg.gg                                                <  2:2397989/101‑1 (MQ=255)
                                                       ggcGAGGTCCCGGGACACATTGGCCCGGTGCCCGGCGAGGCCCCAGAGGCCCT.GGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCg.ggg                                               <  2:942403/99‑1 (MQ=17)
                                                       gCCGGGGTCCCGCGACACATGGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCg.ggg                                               <  1:3582190/101‑1 (MQ=255)
                                                         cGAGGTCCCGCGGCACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCg                                             <  2:2903695/101‑1 (MQ=255)
                                                          gAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCC.GGGGgg                                            >  1:3247506/1‑101 (MQ=255)
                                                           gggccccgcgCCACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCggg                                           <  1:4024309/95‑1 (MQ=14)
                                                           aGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCACTCCCG.CCCCCG.GGGGggg                                           >  1:2048410/1‑101 (MQ=17)
                                                            ggccccgcgACACATGGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGa                                          <  1:3020758/96‑1 (MQ=255)
                                                            ggTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCCCG.CCGCCC.GGGCCGGa                                          >  2:700704/1‑101 (MQ=255)
                                                            gcccccgcgACACATTGGCCCCGTGCCCGCCGAGGGCCCAGGGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGa                                          <  2:973801/96‑1 (MQ=255)
                                                             gTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCG.GGGCggggg                                         >  1:641108/1‑99 (MQ=255)
                                                                ccgcgCCACATTGCCCCCGTGCCCGCCGGGGCCCCAGAGGCCCC.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCa                                      <  1:87331/100‑1 (MQ=17)
                                                                aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAATCGCG.CCCCCC.GGGCGGGACGCa                                      >  2:2858776/1‑101 (MQ=255)
                                                                 cgcgCCACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAg                                     <  2:1481594/101‑1 (MQ=255)
                                                                   cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCGCCC.GGGGGGGGCGCCggg                                   >  2:2254182/1‑100 (MQ=17)
                                                                   cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCC.GGGCGGGGCGCCGGc                                   >  2:4335118/1‑101 (MQ=255)
                                                                       acaTTGCCCCCGTGACCGCCGAGGCCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTcc                               <  2:1203995/101‑1 (MQ=255)
                                                                        caTGGGCCCCGTGCCCGCCGGGGGCCCAGGGCCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTctg                              <  2:1313447/101‑3 (MQ=14)
                                                                          tgggCCCCGTGCCCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCcgc                            <  1:531948/99‑1 (MQ=255)
                                                                           tGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCG.GGGGGGGGCGCCGGCCTCCcccg                           >  2:608999/1‑101 (MQ=14)
                                                                           tGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCACTCGCG.CCGCCC.GGGGGGGGCGCAGGGCCCCcgcg                           >  2:1652486/1‑101 (MQ=14)
                                                                            ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCCCG.CCGCCGCGGGCGGGCCGC.GGACTCCcccgc                          >  2:2196176/1‑101 (MQ=14)
                                                                                 cgggACCCCCGAGGGCCCAAAGGCCCT.CGGGCAGCGCCCGCCCCGCCCATGGCGGGCGC.GTCGCG.CCCACC.CGGCCGGGCGCGTGCCCCCGgcgcgcccgt                    >  2:143807/4‑97 (MQ=11)
                                                                                  gagACCGCGGAGGCCCGAG.GGCCCTGCGGCTGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTgg                    <  2:614406/99‑1 (MQ=17)
                                                                                  gTGACCGCCGGGGGCCCAAAGGCCCT.CGGCCTGCGCCCGCCCCGCCATCGGCGGCCGGCATCGCGCCCGCCC.GGGCGGGGGGCCGGCCCccccgtgccttg                     >  1:550959/1‑92 (MQ=2)
                                                                                  gTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCC.GGGCGGGGCGCCGGCCTCCCCCCCGCCTgg                    >  2:1162931/1‑101 (MQ=11)
                                                                                  gTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCCCG.CCGCCC.GGGCGGGGCGCAGGCCCCCCCCGCGCTTgg                    >  2:1333705/1‑101 (MQ=14)
                                                                                   tGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCCCCG.GGGGGGGACGCAGGCCCCCCGCGCGCCTggg                   >  2:1222299/1‑101 (MQ=17)
                                                                                    gcccgccgGGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                  <  1:2098964/99‑1 (MQ=255)
                                                                                    gACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGGGCCCGCCCCGCCCATGGCGGGGGCATTCCCG.CCGCCG.GGGGGGGGCGCATGCCTCCCCCGCGCCTgggg                  >  1:2523854/1‑101 (MQ=14)
                                                                                      ccgccgGGGCCCCAGAGCCCCT.CGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAg                <  1:1088346/101‑1 (MQ=18)
                                                                                       cgccgGGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAgg               <  1:2466099/101‑1 (MQ=255)
                                                                                       cgccgAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCCCG.CCCCCC.GGGCGGGGCGCCGGCCCCCCCCCCGCCTGGGGGgg               >  1:2607114/1‑101 (MQ=11)
                                                                                       cgccgAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGGCCCCCGCGCGCTTCGGGCgg               >  2:2322445/1‑101 (MQ=17)
                                                                                         ccgAGGGCCCCAAGGCACT.CGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTTTGCG.CCCCCG.GGGGGGGGCGCCGGCCTCCTCCCCGCGTGGGGGGGCg             >  1:3161360/1‑101 (MQ=14)
                                                                                          cgAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCC.GGGGGGGGCGCAGGCCTCCCGCGCGCTTGGGGGGGCgg            >  2:3137360/1‑100 (MQ=17)
                                                                                          cgAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCACTCGCG.CCCCCC.GGGCGGGGCCCCGGCCCCCCCCCCGCCTCGGGGGGCgg            >  1:2571930/1‑100 (MQ=11)
                                                                                           gAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCGCCC.GGGCGGGGCGCAGGCCCCCCCCGCGCTTGGGGGGGCggt           >  1:765867/1‑99 (MQ=14)
                                                                                              ggCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCG.GGGCGGGGCGCAGGGCCCCCGCCCGCTTGGGGGGGCGGTcct        >  2:3637615/1‑99 (MQ=14)
                                                                                              ggCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGGCGCAGGCCTCCCGCGCGCCTGGGGGGGCGGTCtt        >  1:3933078/1‑101 (MQ=17)
                                                                                               gCCCAGGGGCCCC.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg       <  1:1222392/101‑1 (MQ=255)
                                                                                               gCCCAGGGCCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg       <  2:750134/101‑1 (MQ=255)
                                                                                                 ccAGGGGCCCC.CGCCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTc     <  2:2589402/101‑1 (MQ=21)
                                                                                                   agagCCCCG.CGGCCTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCAc   <  1:781947/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
CAACCGCAGCCGTCTGGATCTGTGACGGCGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCT.CGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCG.GGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACC  >  NC_014034/1612298‑1612498

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG