Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,668,401	G→C	100%	intergenic (‑66/+16)	ilvH ← / ← ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,668,401	0	G→C	100.0%	73.5	20	intergenic (‑66/+16)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (20/0):  ref base (0/0):  total (20/0)

GCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668309‑1668490                                                                                             |                                                                                          gCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGc                                                                                 >  1:19248/1‑101 (MQ=255)                             gCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgccgc                                                     >  1:1090011/1‑101 (MQ=255)                              cccTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCcgcg                                                    >  2:4127518/1‑101 (MQ=255)                                      cTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCt                                            >  2:2241396/1‑101 (MQ=255)                                                     tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCGGCCGCGGAAGGCCTCGGCATCGGCCgagg                             >  1:2146905/1‑100 (MQ=25)                                                       tCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagc                           >  2:3189258/1‑101 (MQ=255)                                                           gCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAgcagca                       >  1:4204179/1‑101 (MQ=255)                                                               gAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTc                   >  2:215345/1‑101 (MQ=255)                                                                aaaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCg                  >  2:918415/1‑101 (MQ=255)                                                                    atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt              >  2:4285075/1‑101 (MQ=255)                                                                      cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc            >  1:2400103/1‑101 (MQ=255)                                                                      cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc            >  1:2367441/1‑101 (MQ=255)                                                                      cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc            >  2:2436985/1‑101 (MQ=255)                                                                        cGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCgg          >  1:390574/1‑101 (MQ=255)                                                                        cGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCgg          >  1:2193568/1‑101 (MQ=255)                                                                         ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGCAGGCCTCGGCATCGGCCGCGAGCAGCATTTCGTTATGCGGc         >  2:4422949/1‑101 (MQ=38)                                                                          gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  2:2322072/1‑101 (MQ=255)                                                                               tCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCg   >  1:4432606/1‑101 (MQ=255)                                                                                ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:2312698/1‑101 (MQ=255)                                                                                ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:1366344/1‑101 (MQ=255)                                                                                             |                                                                                          GCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668309‑1668490 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG