Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,733 C→T 100% S477N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,7330C→T100.0% 907.3 227S477N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (112/115):  ref base (0/0):  total (112/115)

Only 100 of 239 total aligned reads displayed.

CCAGTTTCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830
                                                                                                   |                                                                                                 
ccAGTTTCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTg                                                                                                 <  1:2701413/101‑1 (MQ=255)
   gTTTCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTc                                                                                              >  2:1832383/1‑101 (MQ=255)
    tttCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCa                                                                                             <  1:927191/101‑1 (MQ=255)
    tttCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCACCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGGCGTTCa                                                                                             >  1:3444359/1‑101 (MQ=255)
     ttCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCGCTGCCGCACCATGCCCAGCCGCTCGTTGTTCAg                                                                                            >  1:3788328/1‑101 (MQ=255)
           aaaTCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      <  1:347633/101‑1 (MQ=255)
           aaaTCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      >  2:2949921/1‑101 (MQ=255)
            aaTCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAAc                                                                                     <  2:703147/101‑1 (MQ=255)
               cGGGCGGGCTTTCC.GACCAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGc                                                                                  <  1:4105708/101‑1 (MQ=255)
               cGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGc                                                                                  >  1:4185332/1‑101 (MQ=255)
                 ggCAGGCTTTCC.GCCCAGCTTTGCAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                <  1:990680/101‑1 (MQ=255)
                   ggggCTTTCC.GACCAGCTTGGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGa                                                                              <  2:1723213/99‑1 (MQ=255)
                   gcagttttCCGGACCGGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTg                                                                               <  2:843899/96‑1 (MQ=255)
                      gttttCC.GACCAGCTGTGCGAATAACGCCCGCCATGCAGCGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCg                                                                           <  1:543857/99‑1 (MQ=255)
                       cTTTCC.GACCAGCTTTGCAAATAACCCCCGCCATGAAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgg                                                                          <  2:3164785/101‑1 (MQ=255)
                        tttCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCggg                                                                         >  2:1214332/1‑101 (MQ=255)
                         ttCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgggg                                                                        >  1:2320410/1‑101 (MQ=255)
                          ttcggcccAGCTTGGCGAATAACCCCCCACAGTCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgggg                                                                        <  1:1767232/95‑1 (MQ=255)
                          tCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGt                                                                       <  1:1257317/101‑1 (MQ=255)
                          tCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGt                                                                       >  2:1058972/1‑101 (MQ=255)
                          tCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGt                                                                       >  1:1511530/1‑101 (MQ=255)
                           cc.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      >  1:2859916/1‑101 (MQ=255)
                           cc.GACCAGCTTGGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      <  2:2748801/101‑1 (MQ=255)
                            c.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGc                                                                     >  1:1865352/1‑101 (MQ=255)
                            c.GACCAGCTTGGCAAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGc                                                                     <  1:848989/101‑1 (MQ=255)
                              gcccAGCTTTGCAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCg                                                                    <  1:3169443/99‑1 (MQ=255)
                              gACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGAGGCGCg                                                                    >  1:782545/1‑101 (MQ=255)
                               aCCAGCTTTGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCgg                                                                   <  2:3904878/101‑1 (MQ=255)
                                ccAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGa                                                                  <  1:350225/101‑1 (MQ=255)
                                ccAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGa                                                                  <  1:1429620/101‑1 (MQ=255)
                                   gCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACt                                                               >  2:2556222/1‑101 (MQ=255)
                                   gCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACt                                                               >  2:778976/1‑101 (MQ=255)
                                   gCTTTGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACt                                                               <  1:2350825/101‑1 (MQ=255)
                                     ttggcaaaTACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             <  2:143436/95‑1 (MQ=255)
                                     ttggCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             <  2:1854994/98‑1 (MQ=255)
                                        ccaaaTACCCCCCGCCATGCAGCGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTc                                                          <  2:4276482/98‑1 (MQ=255)
                                         cGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCg                                                         >  2:372560/1‑101 (MQ=255)
                                          gAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGc                                                        >  2:29800/1‑101 (MQ=255)
                                          gAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGc                                                        >  2:626492/1‑101 (MQ=255)
                                           aaaaccccccgccATGCCGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       <  2:2886167/94‑1 (MQ=255)
                                           aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  2:4104987/1‑101 (MQ=255)
                                             tacccccccccACGCAGAAGCCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     <  2:3509664/96‑1 (MQ=255)
                                             tAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     >  1:826847/1‑101 (MQ=255)
                                             tAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     >  2:3950839/1‑101 (MQ=255)
                                             aaaCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     <  2:911479/100‑1 (MQ=255)
                                                 gccagccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 >  2:420577/1‑101 (MQ=255)
                                                 ccccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  2:3788075/100‑1 (MQ=255)
                                                  cccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCa                                                >  2:326543/1‑101 (MQ=255)
                                                   ccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               >  2:3036427/1‑101 (MQ=255)
                                                   ccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               <  2:1701574/101‑1 (MQ=255)
                                                   cccccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               <  1:3699032/98‑1 (MQ=255)
                                                    cgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAtt                                              >  2:3706109/1‑101 (MQ=255)
                                                    cgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAtt                                              >  2:1568636/1‑101 (MQ=255)
                                                     gccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             >  2:3879169/1‑101 (MQ=255)
                                                       cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  2:3577667/1‑101 (MQ=255)
                                                       cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           <  2:577922/101‑1 (MQ=255)
                                                         tGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTg                                         >  1:3492748/1‑101 (MQ=255)
                                                          gcagcgGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        <  1:1015147/101‑1 (MQ=255)
                                                            agcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCAt                                      >  2:4149511/1‑101 (MQ=255)
                                                            agcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCAt                                      <  2:2822920/101‑1 (MQ=255)
                                                                   ccTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatc                               <  2:2186951/101‑1 (MQ=255)
                                                                    cTGCCACTGCCGCCCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              <  1:3043527/101‑1 (MQ=255)
                                                                    cTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              <  1:4414168/101‑1 (MQ=255)
                                                                     tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             >  2:1346362/1‑101 (MQ=255)
                                                                     tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  1:1832507/101‑1 (MQ=255)
                                                                     tGCCACGGCCGCCCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  1:3929457/101‑1 (MQ=255)
                                                                      gCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGc                            >  1:1032307/1‑101 (MQ=255)
                                                                      gCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGc                            >  1:4278134/1‑101 (MQ=255)
                                                                       ccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCATcc                           <  1:2556385/101‑1 (MQ=255)
                                                                       ccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGcc                           >  2:1604009/1‑101 (MQ=255)
                                                                       ccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGcc                           <  2:1511425/101‑1 (MQ=255)
                                                                       ccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGcc                           <  1:668199/101‑1 (MQ=255)
                                                                        cACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCa                          <  2:2435706/101‑1 (MQ=255)
                                                                         ccTGCCGCACCAGGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCATCCAg                         <  2:3768288/100‑1 (MQ=255)
                                                                          cTGCCGCGCCATGCCCAGCCGCTCGTTGTTGAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCGGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        <  1:3989343/101‑1 (MQ=255)
                                                                          cTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        >  1:3641905/1‑101 (MQ=255)
                                                                           tGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGa                       >  2:1137963/1‑101 (MQ=255)
                                                                            gCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGAc                      <  1:237193/101‑1 (MQ=255)
                                                                              cGCCCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGc                    <  1:1648167/101‑1 (MQ=255)
                                                                              cGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGc                    >  1:2466025/1‑101 (MQ=255)
                                                                              cGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGc                    >  1:2787081/1‑101 (MQ=255)
                                                                                  ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  1:1571548/101‑1 (MQ=255)
                                                                                   cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               >  2:4307213/1‑101 (MQ=255)
                                                                                   cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               <  1:626560/101‑1 (MQ=255)
                                                                                    aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              >  2:2351887/1‑101 (MQ=255)
                                                                                     tGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCg             <  2:2065598/101‑1 (MQ=255)
                                                                                     tGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCg             <  2:2322138/101‑1 (MQ=255)
                                                                                      gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  1:1630132/1‑101 (MQ=255)
                                                                                      gCCCAGCCGCTCGTTGTCCAGGCTGAACTGCTTGACCGGGGGGCGGGACTGCGGCGCCGTGCCCACTTCCTGCCTGCGCCTCCGCCCGGACGccccgcccg            >  2:2601013/1‑94 (MQ=255)
                                                                                        ccAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCg          <  1:1059060/101‑1 (MQ=255)
                                                                                         cAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGa         <  1:1381216/101‑1 (MQ=255)
                                                                                          aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  2:470015/101‑1 (MQ=255)
                                                                                          aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  2:1907182/101‑1 (MQ=255)
                                                                                          aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  1:2874552/101‑1 (MQ=255)
                                                                                            ccGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGt      >  2:3463417/1‑101 (MQ=255)
                                                                                             cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  1:2034046/101‑1 (MQ=255)
                                                                                             cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     >  1:3220287/1‑101 (MQ=255)
                                                                                             cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  1:2925945/101‑1 (MQ=255)
                                                                                             cgctcggtGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  1:738620/101‑1 (MQ=255)
                                                                                                tcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAt  >  2:3448033/1‑101 (MQ=255)
                                                                                                   |                                                                                                 
CCAGTTTCACGAAATCGGGCAGGCTTTCC.GACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG