Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 702.7 185K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (117/68):  ref base (0/0):  total (117/68)

Only 100 of 211 total aligned reads displayed.

GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCATT  >  NC_014034/1668918‑1669114
                                                                                                  |                                                                                                  
gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                                  <  1:2020289/101‑1 (MQ=255)
 cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                                                 <  1:3659491/101‑1 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                                >  1:1705464/1‑101 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                               <  2:599051/101‑1 (MQ=255)
     tCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGc                                                                                             >  2:4376518/1‑101 (MQ=255)
       ttccgatctaaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                                           <  1:1226836/92‑1 (MQ=255)
       ggCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                                           >  1:655859/1‑101 (MQ=255)
        gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                          <  1:2938733/101‑1 (MQ=255)
         cgcCTCGAAGCCCAGATATTGCGCCGCCCACAGCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                                         <  2:2786875/101‑1 (MQ=255)
         cgcCTCGAAGCCCAGATACTGCGCCGCCCACATCTGCTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGTCCTCCAGCCGCTCCa                                                                                         <  1:2011116/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                        <  2:4447190/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                        <  2:2353340/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                        >  1:4350649/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcgcct                                                                                   >  2:3690253/1‑99 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcagat                                                                                   >  2:2406768/1‑97 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                   >  2:4003389/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                   >  1:4275762/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                   >  1:1020765/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGACCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                   <  1:2724799/101‑1 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatt                                                                                  >  2:1327346/1‑100 (MQ=255)
                aaGCCCAGATATGGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                  >  1:2429557/1‑101 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACTTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                 <  2:3072815/101‑1 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                 >  2:372653/1‑101 (MQ=255)
                   cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                               >  1:1537924/1‑101 (MQ=255)
                    ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                              >  1:3979815/1‑101 (MQ=255)
                    ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                              <  1:3753505/101‑1 (MQ=255)
                    ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                              <  2:3897415/101‑1 (MQ=255)
                    ccAGATATTCGGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                              <  2:1077992/101‑1 (MQ=255)
                     cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                                             <  2:2900584/101‑1 (MQ=255)
                     cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                                             >  1:413982/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                         <  1:2648858/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                         <  1:1629775/101‑1 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGCGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                      <  1:3109239/101‑3 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                                   >  1:4092692/1‑101 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                                   <  2:4065624/101‑1 (MQ=255)
                                ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                                                  >  1:1005268/1‑101 (MQ=255)
                                 cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                                                 >  1:3447490/1‑101 (MQ=255)
                                  gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                                                <  1:586832/101‑1 (MQ=255)
                                   cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                                               >  2:2503783/1‑101 (MQ=255)
                                     cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                                             <  2:3979559/101‑1 (MQ=255)
                                      acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                            >  1:4383546/1‑101 (MQ=255)
                                      acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                            <  2:482136/101‑1 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                      <  1:4027091/101‑1 (MQ=255)
                                              tGCTGGCCGACTTGGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                                                    <  1:1390728/101‑1 (MQ=255)
                                              tGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCggg                                                    >  2:2146148/1‑100 (MQ=255)
                                               gCTGGCCGCCTTCGGTGGTGATGTAGCGGTTGCGCCCCTGGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtg                                                   <  2:2026608/101‑1 (MQ=255)
                                                gtGGCCGACTTCGGTGGTGATTTAGCGGTTGCCCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                                                  <  1:2761773/100‑1 (MQ=255)
                                                cTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                                                  >  1:1830653/1‑101 (MQ=255)
                                                 tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                 >  1:876329/1‑101 (MQ=255)
                                                 gggCCGACTTGGGTGGTGATGTAGCGGTTGCGCCCCTTGTTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                 <  2:3214571/100‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                                >  1:3485072/1‑101 (MQ=255)
                                                   gCCGACTTCGGGGGTTATGTGGGGGTTGCCCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                                               <  1:220286/101‑1 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                             >  2:3421715/1‑101 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGGGTAGGCg                                             >  1:1102919/1‑101 (MQ=255)
                                                     cGACTTCGGGGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATTTTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                             <  2:3361566/101‑1 (MQ=255)
                                                      gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                            >  2:2930201/1‑101 (MQ=255)
                                                       ccTTCGGTGGTGATGTGGGGGTTGCGCCCCTGGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                           <  2:220493/100‑1 (MQ=255)
                                                       aCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                           >  1:2739043/1‑101 (MQ=255)
                                                         ttgggtggtGATGTAACGTTTGCGCCCTTGGGTCGGGGCCCCCAGCCGCCCCAGCGCATATTGGGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                         <  1:3690506/98‑1 (MQ=255)
                                                          tgggtggtGATGTAGGGGTTGCCCCCTTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                                        <  2:3779180/99‑1 (MQ=255)
                                                           cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                       >  1:1799467/1‑101 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                      >  2:264252/1‑101 (MQ=255)
                                                             gtggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg                                     >  1:3871944/1‑101 (MQ=255)
                                                              gggtgAGGTGGCGGTGGCGCCCCTGGGTCATGCCCGCTAGCCTCTCCAGCGCATATTGCGGTTGGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                    <  2:1785609/100‑1 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                   >  2:3876849/1‑101 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                   >  1:2067962/1‑101 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                   <  1:3996412/101‑1 (MQ=255)
                                                                    tGTAGGGGTTGCCCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                              <  1:135625/101‑1 (MQ=255)
                                                                       cgCGGTTGCGCCCTTTGTTCGGGGCCCCCGGCCGCCCGAGCGATTATTGTGGTTGGATGATGCTGTCGGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                           <  2:1537819/100‑1 (MQ=255)
                                                                        gCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTcc                          >  1:1206344/1‑101 (MQ=255)
                                                                          ggttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGCAGGCGCGGCAGCGGATCTTCTTCGAc                        >  2:723356/4‑101 (MQ=255)
                                                                          ggttGCGCCCCTGGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAc                        <  1:2397131/98‑1 (MQ=255)
                                                                            ttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                      >  2:673741/2‑101 (MQ=255)
                                                                            ttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                      >  1:4230157/2‑101 (MQ=255)
                                                                             tGCGCCCTTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                     <  2:1675934/101‑1 (MQ=255)
                                                                             tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                     >  1:407077/1‑101 (MQ=255)
                                                                               cgcCCTTGGGCCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                   <  2:302373/101‑1 (MQ=255)
                                                                               cgcCCCTTGGTCGGCCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                   <  2:1514811/101‑1 (MQ=255)
                                                                               ccccctTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                   <  1:4044786/99‑1 (MQ=255)
                                                                               ccccccTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                   <  1:3294192/99‑1 (MQ=255)
                                                                                gcCCCTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGTTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                  <  1:1839528/101‑1 (MQ=255)
                                                                                cccctTTGGCCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                  <  2:282866/100‑1 (MQ=255)
                                                                                cccccTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGTTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                  <  1:2503946/100‑1 (MQ=255)
                                                                                cccccTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                  <  1:337223/100‑1 (MQ=255)
                                                                                 ccccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGa                 >  2:3528906/1‑101 (MQ=255)
                                                                                  ccttgggTCGGGGCCTCCGGCCGCTCCAGCGAATTTTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt                <  2:1816543/96‑1 (MQ=255)
                                                                                  cccTTGGTGGGCGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt                <  2:173860/101‑1 (MQ=255)
                                                                                    cTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt              >  2:87588/1‑101 (MQ=255)
                                                                                    cTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt              >  1:1891557/1‑101 (MQ=255)
                                                                                     ttGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt             >  1:225990/1‑101 (MQ=255)
                                                                                     tgggTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCAGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt             <  2:2274527/99‑1 (MQ=255)
                                                                                       ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGc           >  2:4210821/1‑101 (MQ=255)
                                                                                       ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGc           >  1:762902/1‑101 (MQ=255)
                                                                                       ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGc           >  2:738793/1‑101 (MQ=255)
                                                                                       ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCCTCTTCCACTCCTCGATCTTGc           >  1:2572013/1‑101 (MQ=255)
                                                                                        gTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcc          >  2:2436568/1‑101 (MQ=255)
                                                                                           gggcccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCcca       <  1:2992990/97‑1 (MQ=255)
                                                                                              gCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccacca    >  1:1825375/1‑101 (MQ=255)
                                                                                              gCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccacca    >  2:121861/1‑101 (MQ=255)
                                                                                               ccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCGCACCCGG.GTAGGCGGGGCAGCGGATCTTCTTCCCCTCCTCGGTCTTGCCCCACCAtt  >  1:692534/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCATT  >  NC_014034/1668918‑1669114

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG