Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,179 A→C 6.0% intergenic (‑107/+118) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1790A→C 6.0% 20.6 ‑3.1 0.0206intergenic (‑107/+118)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (9/4):  ref base (77/116):  total (86/120)
Fisher's exact test strand distribution p-value = 4.54e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283
                                                                                                   |                                                                                                        
ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTc                                                                                                      >  1:4207815/1‑101 (MQ=255)
 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTca                                                                                                     <  1:322824/101‑2 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCG.TTTCGGCCAATTcgccc                                                                                                  >  2:1407532/1‑97 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAccc                                                                                                 <  1:1958533/101‑1 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAccc                                                                                                 >  1:204179/1‑101 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAcccc                                                                                                <  1:1261833/101‑2 (MQ=37)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAcccc                                                                                                >  1:490578/1‑100 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAcccc                                                                                                >  2:4273049/1‑100 (MQ=255)
      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAccccg                                                                                               >  2:2570260/1‑99 (MQ=255)
      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGG.CCAATTCAccccg                                                                                               >  1:3080434/1‑99 (MQ=255)
        ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTTCGC.CAATTcacccccgg                                                                                              >  2:869806/1‑93 (MQ=255)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGa                                                                                             >  1:2136846/1‑101 (MQ=255)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGat                                                                                            >  1:2192208/1‑100 (MQ=37)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGag                                                                                            >  1:156649/1‑100 (MQ=37)
           tctttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGatg                                                                                           <  2:2056095/98‑3 (MQ=25)
           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGatg                                                                                           <  2:3445434/101‑3 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGatgttt                                                                                        >  2:776293/1‑96 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGatgttt                                                                                        >  1:2409147/1‑96 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGatgttt                                                                                        <  1:4368191/101‑6 (MQ=25)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       <  2:3533268/101‑1 (MQ=255)
               cgAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       >  1:2432460/2‑101 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTgg                                                                                      >  2:4292133/1‑101 (MQ=25)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCA.TTTCGGCCAATTCACCCCGGGATGTTTgg                                                                                      <  1:4280607/101‑1 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGc                                                                                    >  1:2653741/1‑101 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGcc                                                                                   <  1:2834175/101‑1 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCa                                                                                  >  2:255564/1‑101 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCa                                                                                  >  2:4168684/1‑101 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCCCCCCGGGATGTTTGGGCCaag                                                                                >  1:4304143/1‑100 (MQ=21)
                       cgTTGAAGTTCGAAGCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGGCCCTCCTGGCC.TTTCGGCCAATTCCCCCCGGGATGTTTGGGACaagg                                                                               <  2:194412/100‑3 (MQ=16)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                              >  2:3569322/1‑98 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGcccaggtg                                                                             >  1:1016181/1‑95 (MQ=19)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             <  1:808522/101‑5 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             <  1:3938231/101‑5 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            <  1:4215441/101‑6 (MQ=16)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            >  2:1104112/1‑96 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            <  1:2240324/101‑6 (MQ=16)
                           tAATTTCGAATCTTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  2:1708772/101‑7 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  2:2120018/101‑7 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  2:1255025/101‑8 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                       <  2:2837212/101‑1 (MQ=16)
                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCCCCTGGCC.TTTCGGCCAATTCATCCCCGGGTGTTTGGGCCAAGgggaaaaa                                                                        >  1:117431/1‑96 (MQ=255)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                   >  2:3693851/1‑97 (MQ=255)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  <  1:260799/101‑6 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 <  1:4141894/101‑7 (MQ=12)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 >  2:658502/1‑95 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 <  2:3597375/101‑7 (MQ=12)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                >  1:1660196/1‑94 (MQ=255)
                                     tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                               <  2:20199/101‑9 (MQ=255)
                                     tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                               >  2:1856447/1‑93 (MQ=12)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  2:2202451/101‑10 (MQ=9)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              >  2:4387085/1‑92 (MQ=255)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  1:4033649/101‑10 (MQ=9)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                             <  1:571807/101‑11 (MQ=255)
                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                       <  1:2635474/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:923827/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGTGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                    <  2:588840/101‑1 (MQ=255)
                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                   <  2:117410/101‑1 (MQ=9)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                <  1:3147993/101‑1 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                             <  2:3218898/101‑1 (MQ=9)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc                                           <  1:776373/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           >  2:235636/1‑101 (MQ=9)
                                                         ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                         <  1:3694104/101‑1 (MQ=255)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                        >  1:2156471/1‑101 (MQ=9)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                      <  2:156628/101‑1 (MQ=255)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                     <  2:3065772/101‑1 (MQ=255)
                                                                   gaggggcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  2:3136530/92‑1 (MQ=9)
                                                                   gaggggcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  1:4168945/92‑1 (MQ=9)
                                                                    gggggccctcccccACCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  2:3521807/91‑1 (MQ=12)
                                                                    aggggcgcttccccTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  2:1759983/91‑1 (MQ=255)
                                                                    aggggcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  1:4241725/93‑1 (MQ=255)
                                                                     ggggcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                               <  1:1451990/94‑1 (MQ=255)
                                                                       ggcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                             <  1:2976580/96‑1 (MQ=255)
                                                                        gcgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                            <  1:2348519/97‑1 (MQ=255)
                                                                         cgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                           <  2:3560932/98‑1 (MQ=16)
                                                                         cgctGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                           >  1:311941/4‑101 (MQ=16)
                                                                            tGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                        >  2:3732371/1‑101 (MQ=19)
                                                                            tGCCCCTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                        >  2:132586/1‑101 (MQ=255)
                                                                              ccccTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      <  2:909483/101‑1 (MQ=255)
                                                                               cccTCCTGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                     >  1:2066798/1‑101 (MQ=255)
                                                                                  tcctGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                  >  2:1637499/4‑101 (MQ=255)
                                                                                   cctGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                 >  1:4355323/3‑101 (MQ=19)
                                                                                    ctGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgagc                >  2:549434/2‑101 (MQ=16)
                                                                                     tGGCC.TTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc               <  1:3535242/101‑1 (MQ=25)
                                                                                     tGGCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc               >  1:2792553/1‑101 (MQ=255)
                                                                                      ggCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg              >  1:4136000/1‑101 (MQ=255)
                                                                                       gCC.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa             <  1:2173129/101‑1 (MQ=21)
                                                                                        cc.TTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            <  1:1762462/101‑1 (MQ=25)
                                                                                        cc.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            <  2:1016096/101‑1 (MQ=19)
                                                                                        cc.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            <  1:255591/101‑1 (MQ=19)
                                                                                         c.TTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           >  2:3342118/1‑101 (MQ=255)
                                                                                           tttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg          >  1:612002/1‑101 (MQ=19)
                                                                                            ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc         <  1:1594419/101‑1 (MQ=25)
                                                                                            ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc         <  2:3417890/101‑1 (MQ=25)
                                                                                            ttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc         <  2:3656425/101‑1 (MQ=19)
                                                                                             tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg        <  1:761875/96‑1 (MQ=19)
                                                                                              cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc       >  1:3464967/5‑101 (MQ=255)
                                                                                               ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg      >  2:3989351/4‑101 (MQ=255)
                                                                                               ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg      >  2:3793398/4‑101 (MQ=255)
                                                                                                  cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc   >  1:1977741/1‑101 (MQ=255)
                                                                                                   aaTTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc  <  1:4248842/101‑1 (MQ=25)
                                                                                                   |                                                                                                        
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG