Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,181 T→A 5.3% intergenic (‑109/+116) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1810T→A 5.3% 22.4 ‑1.6 ‑0.1207intergenic (‑109/+116)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (79/117):  total (86/121)
Fisher's exact test strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.13e-01

Only 100 of 214 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696082‑1696285
                                                                                                      |                                                                                                        
ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTc                                                                                                        <  1:2138323/101‑1 (MQ=255)
 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTca                                                                                                       <  1:322824/101‑2 (MQ=255)
   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcc                                                                                                    >  1:1591287/1‑101 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCG.TTTCG.GCCAATTcgccc                                                                                                    >  2:1407532/1‑97 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcccc                                                                                                  >  2:4273049/1‑100 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAcccc                                                                                                  <  1:1261833/101‑2 (MQ=37)
      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCAccccg                                                                                                 >  2:2570260/1‑99 (MQ=255)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.G.CCAATTCACCCcgg                                                                                                <  1:2108294/101‑1 (MQ=255)
        ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTTCG.C.CAATTcacccccgg                                                                                                >  2:869806/1‑93 (MQ=255)
        ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCggg                                                                                                <  1:4226843/101‑1 (MQ=37)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGat                                                                                              >  1:2192208/1‑100 (MQ=37)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GACAATTCACCCCGGGat                                                                                              >  2:2531876/1‑100 (MQ=255)
           tctttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatg                                                                                             <  2:2056095/98‑3 (MQ=25)
           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatg                                                                                             <  2:3445434/101‑3 (MQ=255)
             tttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgtt                                                                                           <  2:170732/100‑5 (MQ=255)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgtt                                                                                           <  1:2475399/101‑5 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgttt                                                                                          <  1:4368191/101‑6 (MQ=25)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgttt                                                                                          >  2:776293/1‑96 (MQ=255)
              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGatgaga                                                                                          >  1:2056232/1‑96 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                         >  1:2432459/1‑101 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                         <  1:2823804/101‑1 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                         <  2:3533268/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                        <  2:2238252/101‑1 (MQ=255)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                        >  2:4292133/1‑101 (MQ=25)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCA.TTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                        <  1:4280607/101‑1 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                      >  1:2653741/1‑101 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGcc                                                                                     <  2:3632103/101‑1 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                    >  2:4168684/1‑101 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                    >  2:1175606/1‑101 (MQ=255)
                     tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaa                                                                                   <  1:2238776/101‑1 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCaag                                                                                  >  1:4304143/1‑100 (MQ=21)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                  <  1:4190558/101‑2 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                  <  1:309206/101‑2 (MQ=255)
                       tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCC.CTTCGCC.CAACTCACCCCGGGATGTTTGGGTCaggg                                                                                 >  1:216287/1‑98 (MQ=255)
                       cgTTGAAGTTCGAAGCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGGCCCTCCTGGCC.TTTCG.GCCAATTCCCCCCGGGATGTTTGGGACaagg                                                                                 <  2:194412/100‑3 (MQ=16)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                                >  2:3569322/1‑98 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGcccaggtg                                                                               >  1:1016181/1‑95 (MQ=19)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                               <  1:808522/101‑5 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                               <  1:2871673/101‑5 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                             <  2:2120018/101‑7 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                         <  2:2837212/101‑1 (MQ=16)
                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCCCCTGGCC.TTTCG.GCCAATTCATCCCCGGGTGTTTGGGCCAAGgggaaaaa                                                                          >  1:117431/1‑96 (MQ=255)
                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCC.TTTTG.GCCCATTCCACCCGGGATGTTTGGGGCaaggtggaaacc                                                                         >  1:3515363/1‑91 (MQ=255)
                                tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                      >  1:2768825/1‑98 (MQ=255)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                     >  2:3693851/1‑97 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  2:3597375/101‑7 (MQ=12)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   >  2:658502/1‑95 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  1:4141894/101‑7 (MQ=12)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  1:3599323/101‑7 (MQ=12)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  1:4212137/1‑94 (MQ=255)
                                     tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                 <  2:20199/101‑9 (MQ=255)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                <  1:4995/101‑10 (MQ=9)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                >  2:4387085/1‑92 (MQ=255)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                <  2:2202451/101‑10 (MQ=9)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                <  1:195903/101‑10 (MQ=9)
                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         <  1:2635474/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                       <  1:923827/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGTGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                      <  2:588840/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                      <  2:1074620/101‑1 (MQ=255)
                                                 ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGGTGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                   >  1:1685177/1‑101 (MQ=9)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                  <  1:3147993/101‑1 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  2:3218898/101‑1 (MQ=9)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              <  1:1991875/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc                                             <  1:776373/101‑1 (MQ=255)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                          <  1:1354249/101‑1 (MQ=255)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                          <  1:997578/101‑1 (MQ=255)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  1:3959394/101‑1 (MQ=9)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                        <  2:156628/101‑1 (MQ=255)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                        >  1:2298867/1‑101 (MQ=12)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       >  1:3065993/1‑101 (MQ=9)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:3065772/101‑1 (MQ=255)
                                                                   gaggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                   <  1:4168945/92‑1 (MQ=9)
                                                                   gaggggcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                   <  2:3136530/92‑1 (MQ=9)
                                                                    gggggccctcccccACCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                  <  2:3521807/91‑1 (MQ=12)
                                                                        gcgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                              <  1:1649316/97‑1 (MQ=255)
                                                                         cgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                             >  1:1837180/4‑101 (MQ=16)
                                                                         cgctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                             <  2:3560932/98‑1 (MQ=16)
                                                                           ctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                           >  1:1511444/2‑101 (MQ=16)
                                                                           ctGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                           >  2:1163031/2‑101 (MQ=16)
                                                                            tGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          >  2:3732371/1‑101 (MQ=19)
                                                                            tGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          >  1:60797/1‑101 (MQ=19)
                                                                            tGCCCCTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          >  2:132586/1‑101 (MQ=255)
                                                                             gCCCCTCCCGGCC.TCTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         <  1:3849427/101‑1 (MQ=255)
                                                                              ccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                        <  2:909483/101‑1 (MQ=255)
                                                                              ccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                        <  2:3371320/101‑1 (MQ=255)
                                                                              ccccTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                        <  1:4247761/101‑1 (MQ=255)
                                                                                 cTCCTGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGCGCCAAATACGCCTCTGGCCCGAAGGGCaaccgc                     >  2:1734377/1‑97 (MQ=12)
                                                                                  tcctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                    >  2:1637499/4‑101 (MQ=255)
                                                                                   cctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                   <  1:4433256/99‑1 (MQ=19)
                                                                                   cctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                   >  1:4355323/3‑101 (MQ=19)
                                                                                    ctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc                  >  2:1777345/2‑101 (MQ=19)
                                                                                    ctGGCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgagc                  >  2:549434/2‑101 (MQ=16)
                                                                                      ggCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                >  2:3266173/1‑101 (MQ=255)
                                                                                      ggCC.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                >  1:4136000/1‑101 (MQ=255)
                                                                                        cc.TTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa              <  1:235663/101‑1 (MQ=19)
                                                                                              tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg          <  1:761875/96‑1 (MQ=19)
                                                                                               cggccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgggc         <  1:2560678/97‑1 (MQ=25)
                                                                                                ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg        >  2:3793398/4‑101 (MQ=255)
                                                                                                ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg        >  2:3989351/4‑101 (MQ=255)
                                                                                                ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg        <  1:344247/98‑1 (MQ=25)
                                                                                                      |                                                                                                        
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCC.TTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696082‑1696285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG