Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,894 C→A 3.4% intergenic (‑147/+46) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8940C→A 3.4% 8.2 ‑3.1 ‑0.1206intergenic (‑147/+46)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (81/118):  total (87/119)
Fisher's exact test strand distribution p-value = 4.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.27e-01

Only 100 of 226 total aligned reads displayed.

CAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCC  >  NC_014034/1734795‑1734993
                                                                                                     |                                                                                                     
cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                                                                                       <  1:6809/101‑1 (MQ=255)
cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGACATTTATGGCAAGATGAAACTGC.GGc                                                                                                       >  2:1983972/1‑101 (MQ=255)
  aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                     >  1:176125/1‑101 (MQ=255)
     gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                  <  1:881188/101‑1 (MQ=255)
       tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGGCTTCATc                                                                                                >  1:3027659/1‑101 (MQ=255)
        gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                               >  2:3919126/1‑101 (MQ=255)
         aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAGGGCAAGATGAAACTGG.GGCCTTCATCtt                                                                                              >  1:4032815/1‑101 (MQ=18)
          cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                             <  2:298966/101‑1 (MQ=255)
           gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                            <  2:1001358/101‑1 (MQ=255)
            cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                           >  2:3731071/1‑101 (MQ=255)
            cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                           >  2:2318184/1‑101 (MQ=255)
              gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                         <  2:1363499/101‑1 (MQ=255)
                 tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                      <  1:780942/101‑1 (MQ=255)
                 tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                      <  1:2243487/101‑1 (MQ=255)
                  cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                     <  2:2182938/101‑1 (MQ=255)
                   aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                    <  1:1477346/101‑1 (MQ=255)
                   aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                    >  2:3635487/1‑101 (MQ=255)
                    gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                   <  2:3880693/101‑1 (MQ=255)
                      cGACGGGCCGGGCAGGGCGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                 <  2:4010999/101‑1 (MQ=255)
                       gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                <  2:818815/101‑1 (MQ=255)
                        aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                              <  1:3569792/101‑1 (MQ=255)
                         cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                             >  2:3951608/1‑101 (MQ=255)
                           ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                           <  1:2729597/101‑1 (MQ=255)
                           ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                           <  2:3353257/101‑1 (MQ=255)
                           ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                           <  2:2415989/101‑1 (MQ=255)
                            tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                         >  1:3088440/9‑101 (MQ=2)
                             ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCTGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                         <  2:3414477/101‑1 (MQ=255)
                             ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                         >  1:4062656/1‑101 (MQ=255)
                              cgggcCGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                        <  2:605200/101‑1 (MQ=255)
                               gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                       <  1:2340475/101‑1 (MQ=255)
                               cggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                      >  2:1680161/6‑98 (MQ=2)
                                  cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGG.GGCCTTCATCTTTCCATAAATATCCC.GGGGTGAgggg                                                                    >  2:593023/1‑101 (MQ=14)
                                   agcggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATCTTTATGGCAAGATGAAACCGC.GGCCTTCATCTTGCCATAAATATCCC.GGGTTGTGGGGc                                                                   >  2:4108659/4‑101 (MQ=14)
                                    ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                  <  1:1595696/101‑1 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                 <  2:718689/101‑1 (MQ=255)
                                      ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                                >  1:1256737/1‑101 (MQ=255)
                                           gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGGAGA.Gcccc                                                          >  1:3785200/6‑101 (MQ=255)
                                           gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.Gcccc                                                          <  2:1755298/96‑1 (MQ=255)
                                           gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.Gcccc                                                          <  2:1796018/96‑1 (MQ=255)
                                           gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.Gcccc                                                          <  1:4004288/96‑1 (MQ=255)
                                           gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGA.Gcccc                                                          >  2:2944849/6‑101 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTc                                                      <  2:2059104/100‑1 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTc                                                      <  1:2778194/100‑1 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGA.GCCCCGCTc                                                      >  2:3152/2‑101 (MQ=255)
                                                cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCa                                                     <  1:3400990/101‑1 (MQ=255)
                                                 ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCaa                                                    <  1:4134519/101‑1 (MQ=255)
                                                   cgatcttGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATACATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAAcc                                                  <  2:1441973/95‑1 (MQ=255)
                                                   ccGTCCTGCGGCCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAAcc                                                  <  2:672317/101‑1 (MQ=255)
                                                     gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTTCCCTAAATACCCC.GGGGTGGGGGGGGGACGCCCCGC.CAAccca                                                >  1:3849065/1‑99 (MQ=17)
                                                     gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGa                                                >  1:2403462/1‑101 (MQ=255)
                                                      tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGaa                                               >  1:1882242/1‑101 (MQ=255)
                                                      tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGaa                                               >  2:2600881/1‑101 (MQ=255)
                                                      tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGA.GCCCCGCTCAACCGaa                                               >  1:3595324/1‑101 (MQ=255)
                                                       ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAg                                              >  1:4089099/1‑101 (MQ=255)
                                                          gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATg                                           <  2:275859/101‑1 (MQ=255)
                                                           cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGa                                          >  1:4300716/1‑101 (MQ=255)
                                                            ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGAc                                         >  1:3537586/1‑101 (MQ=255)
                                                               gtCCGCCCGGGATATTGATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATGAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAg                                      <  2:3521351/100‑1 (MQ=255)
                                                               cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAg                                      <  1:2971471/101‑1 (MQ=255)
                                                                tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGa                                     >  1:1956681/1‑101 (MQ=255)
                                                                tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGa                                     >  1:4176925/1‑101 (MQ=255)
                                                                 ccccccGGGATATTTATGGCAAGATGAAACTCC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGAt                                    <  1:3731324/101‑1 (MQ=255)
                                                                  cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATc                                   <  2:3823307/101‑1 (MQ=255)
                                                                  cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATc                                   <  2:4156740/101‑1 (MQ=255)
                                                                  cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATc                                   <  1:1060574/101‑1 (MQ=255)
                                                                   ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATcc                                  <  2:1634766/101‑1 (MQ=255)
                                                                   ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATcc                                  <  1:2176442/101‑1 (MQ=255)
                                                                   ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATcc                                  <  1:1721286/101‑1 (MQ=255)
                                                                    cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcg                                 >  2:1842159/1‑101 (MQ=255)
                                                                    cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcg                                 <  1:2526812/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCGAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCG.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcgc                                <  1:1322551/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcgc                                >  1:2040443/1‑101 (MQ=255)
                                                                      cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcgcg                               >  2:4105563/1‑101 (MQ=255)
                                                                      cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcgcg                               <  1:2884614/101‑1 (MQ=255)
                                                                      cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCcgcg                               >  2:3477773/1‑101 (MQ=255)
                                                                        ggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                             <  2:2570051/101‑1 (MQ=255)
                                                                             tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                        >  1:3518265/1‑101 (MQ=255)
                                                                             tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                        <  2:4366906/101‑1 (MQ=255)
                                                                              ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                       >  1:336629/1‑101 (MQ=255)
                                                                              ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                       >  1:2657265/1‑101 (MQ=255)
                                                                                 tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                    <  2:2745488/101‑1 (MQ=255)
                                                                                 tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                    <  1:2318334/101‑1 (MQ=255)
                                                                                  ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                   >  1:1147208/1‑101 (MQ=255)
                                                                                  ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                   <  1:636576/101‑1 (MQ=255)
                                                                                   gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                  <  2:2484270/101‑1 (MQ=255)
                                                                                    cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                 >  1:393949/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  1:1858252/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  1:2488769/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  2:4084979/1‑101 (MQ=255)
                                                                                       gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat              <  1:3279077/101‑1 (MQ=255)
                                                                                        aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg             <  2:916307/101‑1 (MQ=255)
                                                                                        aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg             >  2:3849105/1‑101 (MQ=255)
                                                                                        aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg             >  1:2753174/1‑101 (MQ=255)
                                                                                         tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga            <  2:3784947/101‑1 (MQ=255)
                                                                                         tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga            <  2:1085723/101‑1 (MQ=255)
                                                                                            aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa         <  1:581625/101‑1 (MQ=255)
                                                                                                gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg     >  1:473150/1‑101 (MQ=255)
                                                                                                   ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc   <  1:3478013/101‑1 (MQ=255)
                                                                                                    gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc  >  2:3394169/1‑101 (MQ=255)
                                                                                                    gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc  >  2:1516729/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCC  >  NC_014034/1734795‑1734993

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG