Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,908 T→G 2.7% intergenic (‑161/+32) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,9080T→G 2.7% 6.4 ‑4.5 ‑0.0219intergenic (‑161/+32)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (99/114):  total (105/114)
Fisher's exact test strand distribution p-value = 1.13e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

Only 100 of 238 total aligned reads displayed.

GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008
                                                                                                      |                                                                                                     
gggTCAGCCGACGGGGCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                       <  1:1876838/99‑1 (MQ=255)
gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                       <  2:1499006/101‑1 (MQ=255)
gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                       <  2:1363499/101‑1 (MQ=255)
 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                                      <  1:993481/101‑1 (MQ=255)
 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                                      <  1:506585/101‑1 (MQ=255)
   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                                    <  2:308137/101‑1 (MQ=255)
   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                                    <  1:780942/101‑1 (MQ=255)
   tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                                    <  1:298829/101‑1 (MQ=255)
    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                                   <  2:2182938/101‑1 (MQ=255)
     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                                  <  1:3747060/101‑1 (MQ=255)
         gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                              <  2:818815/101‑1 (MQ=255)
          aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                            <  1:3569792/101‑1 (MQ=255)
           cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                                           >  2:3951608/1‑101 (MQ=255)
             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                                         <  2:3353257/101‑1 (MQ=255)
             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                                         <  1:2729597/101‑1 (MQ=255)
                cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                      >  2:3917176/1‑101 (MQ=255)
                acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCC.GGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                                     >  2:2963105/7‑99 (MQ=2)
                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                                     <  1:2340475/101‑1 (MQ=255)
                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                                     <  2:707371/101‑1 (MQ=255)
                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                                     <  1:3591111/101‑1 (MQ=255)
                    cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGATTTTTTTGCCAAGAGGAAATGGG.GCCCTTATTTTTCCCAAAAAATTCCC.GGGGGGGggg                                                                                   >  1:1651469/1‑101 (MQ=2)
                    cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                                                  <  1:3699400/101‑1 (MQ=255)
                     agcggggCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATCTTTATGGCAAGATGAAACCGC.GGCCTTCATCTTGCCATAAATATCCC.GGGTTGTGGGGc                                                                                 >  2:4108659/4‑101 (MQ=14)
                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                                 <  2:3302839/101‑1 (MQ=255)
                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                                 >  2:636941/1‑101 (MQ=255)
                       gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                               <  2:718689/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCaga                                                                              <  2:600834/101‑1 (MQ=255)
                             gctgccccccGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                         <  1:4004288/96‑1 (MQ=255)
                             gctgccccccGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                         <  2:1755298/96‑1 (MQ=255)
                                  cccccGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                    >  2:4026470/1‑101 (MQ=255)
                                  cccccGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                    >  2:2585613/1‑101 (MQ=255)
                                   ccccGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                                   <  1:4134519/101‑1 (MQ=255)
                                         ccTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                                             >  1:4089099/1‑101 (MQ=255)
                                          cTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                            <  2:1669373/101‑1 (MQ=255)
                                           tGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAccagatcg                                                           >  1:1442080/1‑95 (MQ=255)
                                            gCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                                          <  2:275859/101‑1 (MQ=255)
                                             cGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATTTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCCCAACCGAAGAGGa                                                         >  2:369374/1‑101 (MQ=25)
                                                aCTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                                      <  1:2196118/101‑1 (MQ=255)
                                                aCTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                                      >  2:2653529/1‑101 (MQ=255)
                                                 gtCCGCCC.GGGATATTGATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATGAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                                     <  2:3521351/100‑1 (MQ=255)
                                                  tCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                                    >  1:4176925/1‑101 (MQ=255)
                                                    ccccc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  2:3823307/101‑1 (MQ=255)
                                                    ccccc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  2:4156740/101‑1 (MQ=255)
                                                    ccccc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                  <  1:1060574/101‑1 (MQ=255)
                                                     cccc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                                 <  1:1721286/101‑1 (MQ=255)
                                                      ccc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                                <  1:2526812/101‑1 (MQ=255)
                                                       cc.GGGATATTTATGGCGAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCG.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               <  1:1322551/101‑1 (MQ=255)
                                                       cc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               <  1:3427108/101‑1 (MQ=255)
                                                       cc.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                               >  1:2040443/1‑101 (MQ=255)
                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                             >  1:2709678/1‑101 (MQ=255)
                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                          >  2:2428676/1‑101 (MQ=255)
                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                       >  1:3518265/1‑101 (MQ=255)
                                                                 ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                      >  1:336629/1‑101 (MQ=255)
                                                                    tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                   <  2:340497/101‑1 (MQ=255)
                                                                    tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                   <  1:3972216/101‑1 (MQ=255)
                                                                    tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                   <  1:2318334/101‑1 (MQ=255)
                                                                     ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                                  <  1:636576/101‑1 (MQ=255)
                                                                     ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                                  >  1:1147208/1‑101 (MQ=255)
                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                               >  2:4084979/1‑101 (MQ=255)
                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                               >  1:2488769/1‑101 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             <  1:3279077/101‑1 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             >  1:2176343/1‑101 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             >  2:569827/1‑101 (MQ=255)
                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat                             >  1:2365201/1‑101 (MQ=255)
                                                                           aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            <  2:916307/101‑1 (MQ=255)
                                                                           aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                            >  2:3849105/1‑101 (MQ=255)
                                                                            tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga                           >  2:2381737/1‑101 (MQ=255)
                                                                            tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga                           <  2:3784947/101‑1 (MQ=255)
                                                                             gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                          >  2:3442067/1‑101 (MQ=255)
                                                                              aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                         >  1:3483368/1‑101 (MQ=255)
                                                                              aaaCTGC.GGCCTTCATCTTCCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                         <  2:1832864/101‑1 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        >  1:3057625/1‑101 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  1:581625/101‑1 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                        <  1:4287936/101‑1 (MQ=255)
                                                                                  tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGGTGATGAGCAAgc                     <  1:2428833/101‑1 (MQ=255)
                                                                                  tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                     >  2:2055504/1‑101 (MQ=255)
                                                                                   gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg                    >  1:2774735/1‑101 (MQ=255)
                                                                                   gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg                    <  1:380006/101‑1 (MQ=255)
                                                                                      ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc                  >  1:2891613/1‑101 (MQ=255)
                                                                                         cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt               >  1:1241489/1‑101 (MQ=255)
                                                                                          ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  2:3551242/101‑1 (MQ=255)
                                                                                          ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              >  2:2566387/1‑101 (MQ=255)
                                                                                          ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt              <  2:4103744/101‑1 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             <  1:1573649/101‑1 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             <  1:1951227/101‑1 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             >  1:2845397/1‑101 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc             >  1:4073105/1‑101 (MQ=255)
                                                                                            cATCTTGCCATAAATATCCC.GGGGTGCGTGGCAGAGCCCCGCCCAACCGAAGATGACAAGATCAGCGGCCCGGTTTTCGCTGAGGAGCAAGGGCCCGTTCg            >  1:1836446/1‑101 (MQ=37)
                                                                                            cATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGTTGAGCAAGCCCCCGTTCg            >  2:1522766/1‑101 (MQ=255)
                                                                                            cATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg            >  2:4235463/1‑101 (MQ=255)
                                                                                            cATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg            >  1:199752/1‑101 (MQ=255)
                                                                                             aTCTTGCCATAAATATTCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           <  2:1772320/101‑1 (MQ=255)
                                                                                             aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           >  2:1423035/1‑101 (MQ=255)
                                                                                             aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           <  2:1100286/101‑1 (MQ=255)
                                                                                             aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           >  1:686801/1‑101 (MQ=255)
                                                                                             aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg           >  1:4349801/1‑101 (MQ=255)
                                                                                              tCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGc          >  2:1977607/1‑101 (MQ=255)
                                                                                                 tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  1:3169663/1‑101 (MQ=255)
                                                                                                 tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg       >  1:3890276/1‑101 (MQ=255)
                                                                                                  gCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt      <  1:137467/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCC.GGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTT  >  NC_014034/1734809‑1735008

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG