Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,371 C→G 9.3% P148R (CCC→CGG)  RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3710C→G 9.3% 22.0 ‑3.8 ‑1.4118P148R (CCC→CGC) RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/10):  ref base (50/57):  total (51/67)
Fisher's exact test strand distribution p-value = 2.25e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.83e-02

Only 100 of 122 total aligned reads displayed.

CGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGG.GCGGTGGTC.CC.CC.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769285‑1769462
                                                                                           |                                                                                              
  gCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGc                                                                                <  2:4306922/101‑1 (MQ=255)
   cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGcc                                                                               >  2:2204905/1‑101 (MQ=255)
   cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGcc                                                                               <  1:3672193/101‑1 (MQ=255)
    gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCt                                                                              <  2:2503078/101‑1 (MQ=255)
    gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCt                                                                              <  2:1157644/101‑1 (MQ=255)
     aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCtt                                                                             >  2:348336/1‑101 (MQ=255)
     aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCtt                                                                             >  2:3603087/1‑101 (MQ=255)
      tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            >  1:2436703/1‑101 (MQ=255)
      tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            <  2:624719/101‑1 (MQ=255)
      tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTg                                                                            >  2:696804/1‑101 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTgg                                                                           >  2:1303048/1‑101 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTgg                                                                           <  1:2685928/101‑1 (MQ=255)
         ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTgggg                                                                         >  2:4337738/1‑101 (MQ=255)
         ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTgggg                                                                         <  1:2880287/101‑1 (MQ=255)
          gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGt                                                                        <  1:3502752/101‑1 (MQ=255)
           aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:3909428/101‑1 (MQ=255)
           aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:2645551/101‑1 (MQ=255)
           aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTc                                                                       <  1:332887/101‑1 (MQ=255)
            ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCg                                                                      <  1:188598/101‑1 (MQ=255)
             cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGa                                                                     <  2:816487/101‑1 (MQ=255)
             cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGa                                                                     <  2:502883/101‑1 (MQ=255)
             cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGa                                                                     <  1:3591616/101‑1 (MQ=255)
             cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGGCGa                                                                     >  1:2167565/1‑101 (MQ=255)
              aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAg                                                                    <  1:19472/101‑1 (MQ=255)
              aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGGGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAg                                                                    >  1:3871982/1‑101 (MQ=255)
               aCTGGATGCGGCCCGCGCGGCGTAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAgg                                                                   <  2:3555509/101‑1 (MQ=255)
                cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgc                                                                  <  1:696884/101‑1 (MQ=255)
                cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:4006280/101‑1 (MQ=255)
                 tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:1252309/1‑101 (MQ=255)
                 tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcg                                                                 >  1:4385138/1‑101 (MQ=255)
                  ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGgcgc                                                                >  1:4068504/1‑101 (MQ=255)
                    aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCtt                                                              >  2:407463/1‑101 (MQ=255)
                      gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:3033246/101‑1 (MQ=255)
                       cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  2:4017831/101‑1 (MQ=255)
                       cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  2:2439398/101‑1 (MQ=255)
                        ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  2:1192476/1‑101 (MQ=255)
                         gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         >  2:1329232/1‑101 (MQ=255)
                         gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGGCGAGGCGCTTCCggg                                                         >  2:12713/1‑101 (MQ=255)
                         gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GCGCG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         <  2:2866950/101‑1 (MQ=255)
                           ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       >  1:179423/1‑101 (MQ=255)
                           ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       >  2:3018912/1‑101 (MQ=255)
                            cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGATCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                      <  1:3801326/101‑1 (MQ=255)
                             gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:3828157/101‑1 (MQ=255)
                              cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:4334009/101‑1 (MQ=255)
                               gcggcgGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGa                                                   >  2:3088838/1‑101 (MQ=255)
                               gcggcgGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGa                                                   <  2:3672848/101‑1 (MQ=255)
                                 ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGGTg                                                 >  2:3748191/1‑101 (MQ=255)
                                 ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  1:3129694/101‑1 (MQ=255)
                                  gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  2:1648139/101‑1 (MQ=255)
                                  gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4159137/101‑1 (MQ=255)
                                  gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4087687/101‑1 (MQ=255)
                                  gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTGTGCCTTGGGGTCGAGGCGCTTCCGGGCACGGATgc                                                >  2:1796125/1‑101 (MQ=255)
                                   cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               >  2:2892623/1‑101 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:1744235/1‑101 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             <  1:3623548/101‑1 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  1:575890/1‑101 (MQ=255)
                                        aTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          >  2:2656019/1‑101 (MQ=255)
                                        aTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  2:609658/101‑1 (MQ=255)
                                         tcgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:407503/101‑1 (MQ=255)
                                         tcggcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  2:1557620/101‑1 (MQ=255)
                                         acggcccgGCTTGGGGCTCAGTAGATAG.CGGGCA.GGGAGCGGTGGAC.CC.GC.GGCGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGGAACGATGCGCTGCtt                                         <  2:1563056/95‑1 (MQ=255)
                                          cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:1005157/1‑101 (MQ=255)
                                          cgtcgCGGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  1:4405441/101‑1 (MQ=255)
                                             cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GGGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:1756316/1‑101 (MQ=255)
                                             cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:840454/1‑101 (MQ=255)
                                             cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:3128591/1‑101 (MQ=255)
                                             cgcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:432728/1‑101 (MQ=255)
                                              gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:2798113/101‑1 (MQ=255)
                                              gcgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    >  1:4338261/1‑101 (MQ=255)
                                               cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  2:1931607/101‑1 (MQ=255)
                                               cgGCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:1235897/101‑1 (MQ=255)
                                                ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  1:1910892/101‑1 (MQ=255)
                                                ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  1:4338007/101‑1 (MQ=255)
                                                ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:1514710/1‑101 (MQ=255)
                                                ggCTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  2:3806012/1‑101 (MQ=255)
                                                  gtttGGGCGCTGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  2:4384866/100‑1 (MQ=255)
                                                  cTTTGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                >  2:3842208/1‑101 (MQ=255)
                                                   tttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               <  2:842451/101‑1 (MQ=255)
                                                    ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  1:375015/1‑101 (MQ=255)
                                                    ttGGGCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              <  2:2066807/101‑1 (MQ=255)
                                                       ggCGCGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                           >  2:3510195/1‑101 (MQ=255)
                                                        gcgcGGTCGATGT.CGGGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCcgcg                          >  2:3926316/1‑101 (MQ=255)
                                                          gcgGTCGCTGTGG.GGCAGGGG.GCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        <  2:953350/101‑1 (MQ=255)
                                                                    gtcgggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:1782373/92‑1 (MQ=255)
                                                                       gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3526385/7‑101 (MQ=255)
                                                                       gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:3113963/95‑1 (MQ=255)
                                                                       gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  1:3954051/7‑101 (MQ=255)
                                                                       gggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:3055196/7‑101 (MQ=255)
                                                                        ggcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg           >  2:184345/6‑101 (MQ=255)
                                                                         gcagggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:1005072/97‑1 (MQ=255)
                                                                           agggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:637801/3‑101 (MQ=255)
                                                                           agggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:1596778/3‑101 (MQ=255)
                                                                            gggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       <  2:2509471/100‑1 (MQ=255)
                                                                            gggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  2:2022841/2‑101 (MQ=255)
                                                                             ggg.gCGGTGGTCGCG.GG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:2644784/101‑1 (MQ=255)
                                                                             ggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:1865525/101‑1 (MQ=255)
                                                                             ggg.gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      >  2:1915178/1‑101 (MQ=255)
                                                                                 gCGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:2587929/101‑1 (MQ=255)
                                                                                 gCGGTGGTC.GC.GCGCG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   >  2:786139/1‑101 (MQ=255)
                                                                                  cGGTGGTCGCG.CG.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:369551/101‑1 (MQ=255)
                                                                                           |                                                                                              
CGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.GGCA.GGG.GCGGTGGTC.CC.CC.CG.GGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769285‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG